Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05317 and H281DRAFT_05318 are separated by 163 nucleotides H281DRAFT_05318 and H281DRAFT_05319 are separated by 183 nucleotides
H281DRAFT_05317: H281DRAFT_05317 - transcriptional regulator, GntR family, at 67,465 to 68,208
_05317
H281DRAFT_05318: H281DRAFT_05318 - MFS transporter, ACS family, glucarate transporter, at 68,372 to 69,721
_05318
H281DRAFT_05319: H281DRAFT_05319 - 5-dehydro-4-deoxyglucarate dehydratase, at 69,905 to 70,822
_05319
Position (kb) 68
69
70 Strain fitness (log2 ratio) -2
-1
0
1
2 at 67.427 kb on + strand at 67.427 kb on + strand at 67.428 kb on - strand at 67.470 kb on + strand at 67.470 kb on + strand at 67.471 kb on - strand at 68.163 kb on + strand at 68.231 kb on + strand at 68.259 kb on - strand at 68.398 kb on + strand at 68.398 kb on + strand at 68.421 kb on + strand at 68.421 kb on + strand at 68.682 kb on + strand, within H281DRAFT_05318 at 68.683 kb on - strand, within H281DRAFT_05318 at 68.884 kb on - strand, within H281DRAFT_05318 at 68.911 kb on - strand, within H281DRAFT_05318 at 68.931 kb on + strand, within H281DRAFT_05318 at 69.028 kb on + strand, within H281DRAFT_05318 at 69.029 kb on - strand, within H281DRAFT_05318 at 69.066 kb on + strand, within H281DRAFT_05318 at 69.066 kb on + strand, within H281DRAFT_05318 at 69.067 kb on - strand, within H281DRAFT_05318 at 69.143 kb on + strand, within H281DRAFT_05318 at 69.169 kb on - strand, within H281DRAFT_05318 at 69.221 kb on + strand, within H281DRAFT_05318 at 69.221 kb on + strand, within H281DRAFT_05318 at 69.237 kb on + strand, within H281DRAFT_05318 at 69.517 kb on - strand, within H281DRAFT_05318 at 69.517 kb on - strand, within H281DRAFT_05318 at 69.543 kb on + strand, within H281DRAFT_05318 at 69.544 kb on - strand, within H281DRAFT_05318 at 69.544 kb on - strand, within H281DRAFT_05318 at 69.622 kb on - strand at 69.638 kb on + strand at 69.639 kb on - strand at 69.646 kb on + strand at 69.647 kb on - strand at 69.733 kb on + strand at 69.733 kb on + strand at 69.734 kb on - strand at 69.740 kb on + strand at 69.740 kb on + strand at 69.741 kb on - strand at 69.741 kb on - strand at 69.741 kb on - strand at 70.173 kb on - strand, within H281DRAFT_05319 at 70.209 kb on + strand, within H281DRAFT_05319 at 70.210 kb on - strand, within H281DRAFT_05319 at 70.395 kb on + strand, within H281DRAFT_05319 at 70.395 kb on + strand, within H281DRAFT_05319 at 70.396 kb on - strand, within H281DRAFT_05319 at 70.479 kb on + strand, within H281DRAFT_05319 at 70.479 kb on + strand, within H281DRAFT_05319 at 70.480 kb on - strand, within H281DRAFT_05319 at 70.639 kb on + strand, within H281DRAFT_05319 at 70.640 kb on - strand, within H281DRAFT_05319
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 67,427 + +1.1 67,427 + -0.6 67,428 - -0.3 67,470 + +0.7 67,470 + -0.7 67,471 - +0.5 68,163 + -0.6 68,231 + +0.3 68,259 - -0.1 68,398 + +0.2 68,398 + -2.5 68,421 + +1.9 68,421 + +0.7 68,682 + H281DRAFT_05318 0.23 +0.2 68,683 - H281DRAFT_05318 0.23 -0.0 68,884 - H281DRAFT_05318 0.38 -0.1 68,911 - H281DRAFT_05318 0.40 -0.1 68,931 + H281DRAFT_05318 0.41 +0.5 69,028 + H281DRAFT_05318 0.49 -0.5 69,029 - H281DRAFT_05318 0.49 -0.7 69,066 + H281DRAFT_05318 0.51 -0.9 69,066 + H281DRAFT_05318 0.51 -0.1 69,067 - H281DRAFT_05318 0.51 -0.3 69,143 + H281DRAFT_05318 0.57 +0.8 69,169 - H281DRAFT_05318 0.59 +1.1 69,221 + H281DRAFT_05318 0.63 -0.6 69,221 + H281DRAFT_05318 0.63 +0.4 69,237 + H281DRAFT_05318 0.64 +0.2 69,517 - H281DRAFT_05318 0.85 -0.2 69,517 - H281DRAFT_05318 0.85 +0.2 69,543 + H281DRAFT_05318 0.87 +0.6 69,544 - H281DRAFT_05318 0.87 -0.9 69,544 - H281DRAFT_05318 0.87 +0.1 69,622 - -0.1 69,638 + -0.8 69,639 - +0.1 69,646 + -0.9 69,647 - +0.3 69,733 + -0.5 69,733 + -0.8 69,734 - -0.6 69,740 + +0.1 69,740 + +0.1 69,741 - -0.6 69,741 - +0.7 69,741 - +0.4 70,173 - H281DRAFT_05319 0.29 +1.2 70,209 + H281DRAFT_05319 0.33 -0.8 70,210 - H281DRAFT_05319 0.33 +0.3 70,395 + H281DRAFT_05319 0.53 -0.2 70,395 + H281DRAFT_05319 0.53 -0.0 70,396 - H281DRAFT_05319 0.53 -0.1 70,479 + H281DRAFT_05319 0.63 +0.2 70,479 + H281DRAFT_05319 0.63 -0.5 70,480 - H281DRAFT_05319 0.63 +0.5 70,639 + H281DRAFT_05319 0.80 -1.1 70,640 - H281DRAFT_05319 0.80 +0.6
Or see this region's nucleotide sequence