Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04967 and H281DRAFT_04968 are separated by 227 nucleotides H281DRAFT_04968 and H281DRAFT_04969 are separated by 312 nucleotides H281DRAFT_04969 and H281DRAFT_04970 overlap by 4 nucleotides H281DRAFT_04970 and H281DRAFT_04971 are separated by 79 nucleotides
H281DRAFT_04967: H281DRAFT_04967 - protein of unknown function (DUF1857), at 150,826 to 151,308
_04967
H281DRAFT_04968: H281DRAFT_04968 - Hemin uptake protein hemP, at 151,536 to 151,799
_04968
H281DRAFT_04969: H281DRAFT_04969 - 4Fe-4S binding domain-containing protein, at 152,112 to 153,518
_04969
H281DRAFT_04970: H281DRAFT_04970 - high-affinity iron transporter, at 153,515 to 154,360
_04970
H281DRAFT_04971: H281DRAFT_04971 - Predicted periplasmic lipoprotein involved in iron transport, at 154,440 to 154,772
_04971
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2 at 151.159 kb on + strand, within H281DRAFT_04967 at 151.248 kb on + strand, within H281DRAFT_04967 at 151.270 kb on + strand at 151.270 kb on + strand at 151.270 kb on + strand at 151.271 kb on - strand at 151.271 kb on - strand at 151.271 kb on - strand at 151.326 kb on + strand at 151.327 kb on - strand at 151.327 kb on - strand at 151.718 kb on + strand, within H281DRAFT_04968 at 151.752 kb on + strand, within H281DRAFT_04968 at 151.752 kb on + strand, within H281DRAFT_04968 at 151.798 kb on - strand at 151.798 kb on - strand at 151.855 kb on + strand at 151.863 kb on - strand at 151.894 kb on - strand at 151.976 kb on - strand at 152.009 kb on + strand at 152.040 kb on - strand at 152.072 kb on - strand at 152.072 kb on - strand at 152.095 kb on + strand at 152.096 kb on - strand at 152.221 kb on + strand at 152.557 kb on + strand, within H281DRAFT_04969 at 152.707 kb on + strand, within H281DRAFT_04969 at 152.828 kb on + strand, within H281DRAFT_04969 at 152.829 kb on - strand, within H281DRAFT_04969 at 152.829 kb on - strand, within H281DRAFT_04969 at 152.853 kb on - strand at 152.853 kb on - strand, within H281DRAFT_04969 at 152.853 kb on - strand, within H281DRAFT_04969 at 152.954 kb on - strand, within H281DRAFT_04969 at 153.011 kb on - strand, within H281DRAFT_04969 at 153.011 kb on - strand, within H281DRAFT_04969 at 153.011 kb on - strand, within H281DRAFT_04969 at 153.133 kb on + strand, within H281DRAFT_04969 at 153.133 kb on + strand, within H281DRAFT_04969 at 153.134 kb on - strand, within H281DRAFT_04969 at 153.134 kb on - strand, within H281DRAFT_04969 at 153.134 kb on - strand, within H281DRAFT_04969 at 153.413 kb on - strand at 153.413 kb on - strand at 153.512 kb on + strand at 153.577 kb on - strand at 153.591 kb on + strand at 153.856 kb on - strand, within H281DRAFT_04970 at 154.246 kb on - strand, within H281DRAFT_04970 at 154.346 kb on + strand at 154.347 kb on - strand at 154.347 kb on - strand at 154.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 151,159 + H281DRAFT_04967 0.69 +0.4 151,248 + H281DRAFT_04967 0.87 +0.6 151,270 + -0.7 151,270 + -0.2 151,270 + +0.2 151,271 - -1.6 151,271 - -0.5 151,271 - -0.4 151,326 + +1.3 151,327 - -0.4 151,327 - -0.7 151,718 + H281DRAFT_04968 0.69 +0.1 151,752 + H281DRAFT_04968 0.82 -0.5 151,752 + H281DRAFT_04968 0.82 -1.3 151,798 - -0.5 151,798 - -0.2 151,855 + -0.9 151,863 - -1.4 151,894 - -0.3 151,976 - +2.4 152,009 + +0.5 152,040 - +0.2 152,072 - +0.2 152,072 - -0.7 152,095 + -0.4 152,096 - -0.1 152,221 + -0.5 152,557 + H281DRAFT_04969 0.32 -0.0 152,707 + H281DRAFT_04969 0.42 -0.5 152,828 + H281DRAFT_04969 0.51 -0.1 152,829 - H281DRAFT_04969 0.51 -0.7 152,829 - H281DRAFT_04969 0.51 +0.2 152,853 - -1.2 152,853 - H281DRAFT_04969 0.53 -0.5 152,853 - H281DRAFT_04969 0.53 -1.2 152,954 - H281DRAFT_04969 0.60 -0.5 153,011 - H281DRAFT_04969 0.64 -0.7 153,011 - H281DRAFT_04969 0.64 +0.1 153,011 - H281DRAFT_04969 0.64 -1.1 153,133 + H281DRAFT_04969 0.73 -1.1 153,133 + H281DRAFT_04969 0.73 -1.3 153,134 - H281DRAFT_04969 0.73 -0.2 153,134 - H281DRAFT_04969 0.73 +0.1 153,134 - H281DRAFT_04969 0.73 -1.6 153,413 - -0.2 153,413 - -1.2 153,512 + -0.2 153,577 - -1.4 153,591 + -0.3 153,856 - H281DRAFT_04970 0.40 -0.5 154,246 - H281DRAFT_04970 0.86 -1.1 154,346 + -2.1 154,347 - +0.7 154,347 - -0.4 154,442 - +0.3
Or see this region's nucleotide sequence