Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_04813

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_04811 and H281DRAFT_04812 are separated by 286 nucleotidesH281DRAFT_04812 and H281DRAFT_04813 are separated by 245 nucleotidesH281DRAFT_04813 and H281DRAFT_04814 are separated by 252 nucleotidesH281DRAFT_04814 and H281DRAFT_04815 are separated by 10 nucleotides H281DRAFT_04811: H281DRAFT_04811 - hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 246,631 to 247,491 _04811 H281DRAFT_04812: H281DRAFT_04812 - Rubredoxin, at 247,778 to 247,948 _04812 H281DRAFT_04813: H281DRAFT_04813 - hypothetical protein, at 248,194 to 249,603 _04813 H281DRAFT_04814: H281DRAFT_04814 - putative transcriptional regulator, at 249,856 to 250,434 _04814 H281DRAFT_04815: H281DRAFT_04815 - putative holliday junction resolvase, at 250,445 to 250,885 _04815 Position (kb) 248 249 250Strain fitness (log2 ratio) -2 -1 0 1 2 3at 247.204 kb on + strand, within H281DRAFT_04811at 247.204 kb on + strand, within H281DRAFT_04811at 247.225 kb on + strand, within H281DRAFT_04811at 247.226 kb on - strandat 247.226 kb on - strand, within H281DRAFT_04811at 247.226 kb on - strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.359 kb on + strand, within H281DRAFT_04811at 247.360 kb on - strand, within H281DRAFT_04811at 247.360 kb on - strand, within H281DRAFT_04811at 247.361 kb on + strand, within H281DRAFT_04811at 247.513 kb on - strandat 247.547 kb on + strandat 247.548 kb on - strandat 247.548 kb on - strandat 247.556 kb on + strandat 247.709 kb on - strandat 247.709 kb on - strandat 247.709 kb on - strandat 247.771 kb on + strandat 247.771 kb on + strandat 248.043 kb on + strandat 248.044 kb on - strandat 248.044 kb on - strandat 248.044 kb on - strandat 248.134 kb on + strandat 248.530 kb on - strand, within H281DRAFT_04813at 248.689 kb on + strand, within H281DRAFT_04813at 248.690 kb on - strand, within H281DRAFT_04813at 248.790 kb on - strand, within H281DRAFT_04813at 248.790 kb on - strand, within H281DRAFT_04813at 248.818 kb on + strand, within H281DRAFT_04813at 248.819 kb on - strand, within H281DRAFT_04813at 248.906 kb on - strand, within H281DRAFT_04813at 249.004 kb on + strand, within H281DRAFT_04813at 249.005 kb on - strand, within H281DRAFT_04813at 249.005 kb on - strand, within H281DRAFT_04813at 249.061 kb on + strand, within H281DRAFT_04813at 249.205 kb on + strand, within H281DRAFT_04813at 249.206 kb on - strand, within H281DRAFT_04813at 249.206 kb on - strand, within H281DRAFT_04813at 249.206 kb on - strand, within H281DRAFT_04813at 249.206 kb on - strand, within H281DRAFT_04813at 249.206 kb on - strand, within H281DRAFT_04813at 249.574 kb on - strandat 249.574 kb on - strandat 249.574 kb on - strandat 249.646 kb on + strandat 249.646 kb on + strandat 249.647 kb on - strandat 249.647 kb on - strandat 249.647 kb on - strandat 249.654 kb on + strandat 249.654 kb on + strandat 249.654 kb on + strandat 249.655 kb on - strandat 249.671 kb on - strandat 249.687 kb on + strandat 249.746 kb on - strandat 249.746 kb on - strandat 249.755 kb on + strandat 249.755 kb on + strandat 249.755 kb on + strandat 249.756 kb on - strandat 249.855 kb on + strandat 249.855 kb on + strandat 249.855 kb on + strandat 249.855 kb on + strandat 249.855 kb on + strandat 249.855 kb on + strandat 249.855 kb on + strandat 250.081 kb on + strand, within H281DRAFT_04814at 250.147 kb on + strand, within H281DRAFT_04814at 250.147 kb on + strand, within H281DRAFT_04814at 250.147 kb on + strand, within H281DRAFT_04814at 250.147 kb on + strand, within H281DRAFT_04814at 250.147 kb on + strand, within H281DRAFT_04814at 250.148 kb on - strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.149 kb on + strand, within H281DRAFT_04814at 250.150 kb on - strand, within H281DRAFT_04814

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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247,204 + H281DRAFT_04811 0.67 -0.3
247,204 + H281DRAFT_04811 0.67 +0.1
247,225 + H281DRAFT_04811 0.69 -1.2
247,226 - +3.5
247,226 - H281DRAFT_04811 0.69 -0.5
247,226 - H281DRAFT_04811 0.69 -0.2
247,359 + H281DRAFT_04811 0.85 -0.7
247,359 + H281DRAFT_04811 0.85 -0.2
247,359 + H281DRAFT_04811 0.85 -0.9
247,360 - H281DRAFT_04811 0.85 -0.3
247,360 - H281DRAFT_04811 0.85 -0.5
247,361 + H281DRAFT_04811 0.85 +1.4
247,513 - +0.0
247,547 + -0.7
247,548 - +0.1
247,548 - +0.1
247,556 + +0.3
247,709 - +1.2
247,709 - -1.8
247,709 - +1.0
247,771 + -0.4
247,771 + +0.2
248,043 + -0.0
248,044 - +0.8
248,044 - +0.5
248,044 - -0.4
248,134 + +0.3
248,530 - H281DRAFT_04813 0.24 -0.0
248,689 + H281DRAFT_04813 0.35 -1.4
248,690 - H281DRAFT_04813 0.35 -0.5
248,790 - H281DRAFT_04813 0.42 +1.0
248,790 - H281DRAFT_04813 0.42 -0.7
248,818 + H281DRAFT_04813 0.44 +0.5
248,819 - H281DRAFT_04813 0.44 +0.6
248,906 - H281DRAFT_04813 0.50 +0.2
249,004 + H281DRAFT_04813 0.57 +0.6
249,005 - H281DRAFT_04813 0.58 -0.3
249,005 - H281DRAFT_04813 0.58 +0.5
249,061 + H281DRAFT_04813 0.61 +1.7
249,205 + H281DRAFT_04813 0.72 -0.2
249,206 - H281DRAFT_04813 0.72 +0.2
249,206 - H281DRAFT_04813 0.72 -0.2
249,206 - H281DRAFT_04813 0.72 +0.3
249,206 - H281DRAFT_04813 0.72 -0.3
249,206 - H281DRAFT_04813 0.72 +1.0
249,574 - +0.8
249,574 - +0.5
249,574 - +0.3
249,646 + -0.7
249,646 + +0.2
249,647 - +0.2
249,647 - +0.3
249,647 - -0.4
249,654 + -0.5
249,654 + -0.0
249,654 + +0.2
249,655 - +0.8
249,671 - +0.2
249,687 + -0.1
249,746 - -0.0
249,746 - +0.0
249,755 + -0.3
249,755 + -0.0
249,755 + +0.6
249,756 - +0.3
249,855 + -0.8
249,855 + +2.0
249,855 + +0.5
249,855 + +0.8
249,855 + -0.3
249,855 + -0.4
249,855 + -2.0
250,081 + H281DRAFT_04814 0.39 -0.2
250,147 + H281DRAFT_04814 0.50 +0.3
250,147 + H281DRAFT_04814 0.50 -0.8
250,147 + H281DRAFT_04814 0.50 -0.1
250,147 + H281DRAFT_04814 0.50 -0.1
250,147 + H281DRAFT_04814 0.50 +0.8
250,148 - H281DRAFT_04814 0.50 -1.9
250,149 + H281DRAFT_04814 0.51 -0.0
250,149 + H281DRAFT_04814 0.51 +0.5
250,149 + H281DRAFT_04814 0.51 -0.4
250,149 + H281DRAFT_04814 0.51 +1.0
250,149 + H281DRAFT_04814 0.51 +0.0
250,149 + H281DRAFT_04814 0.51 -0.0
250,149 + H281DRAFT_04814 0.51 +0.3
250,150 - H281DRAFT_04814 0.51 -0.4

Or see this region's nucleotide sequence