Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04808 and H281DRAFT_04809 are separated by 177 nucleotides H281DRAFT_04809 and H281DRAFT_04810 are separated by 17 nucleotides H281DRAFT_04810 and H281DRAFT_04811 are separated by 267 nucleotides
H281DRAFT_04808: H281DRAFT_04808 - chaperonin GroEL, at 242,702 to 244,342
_04808
H281DRAFT_04809: H281DRAFT_04809 - adenylylsulfate kinase, at 244,520 to 245,134
_04809
H281DRAFT_04810: H281DRAFT_04810 - Sulfotransferase family protein, at 245,152 to 246,363
_04810
H281DRAFT_04811: H281DRAFT_04811 - hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 246,631 to 247,491
_04811
Position (kb)
245
246
247 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 244.340 kb on + strand at 244.340 kb on + strand at 244.340 kb on + strand at 244.340 kb on + strand at 244.340 kb on + strand at 244.340 kb on + strand at 244.340 kb on + strand at 244.341 kb on - strand at 244.341 kb on - strand at 244.516 kb on - strand at 244.521 kb on + strand at 244.521 kb on + strand at 244.565 kb on - strand at 244.571 kb on + strand at 244.689 kb on + strand, within H281DRAFT_04809 at 244.689 kb on + strand, within H281DRAFT_04809 at 244.740 kb on + strand, within H281DRAFT_04809 at 244.740 kb on + strand, within H281DRAFT_04809 at 244.740 kb on + strand, within H281DRAFT_04809 at 244.740 kb on + strand, within H281DRAFT_04809 at 244.740 kb on + strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.741 kb on - strand, within H281DRAFT_04809 at 244.742 kb on + strand, within H281DRAFT_04809 at 244.742 kb on + strand, within H281DRAFT_04809 at 244.742 kb on + strand, within H281DRAFT_04809 at 244.742 kb on + strand, within H281DRAFT_04809 at 244.743 kb on - strand, within H281DRAFT_04809 at 244.743 kb on - strand, within H281DRAFT_04809 at 244.743 kb on - strand, within H281DRAFT_04809 at 244.743 kb on - strand at 244.743 kb on - strand, within H281DRAFT_04809 at 244.819 kb on + strand, within H281DRAFT_04809 at 244.819 kb on + strand, within H281DRAFT_04809 at 244.820 kb on - strand, within H281DRAFT_04809 at 244.820 kb on - strand, within H281DRAFT_04809 at 244.820 kb on - strand, within H281DRAFT_04809 at 244.822 kb on + strand, within H281DRAFT_04809 at 244.823 kb on - strand, within H281DRAFT_04809 at 244.828 kb on + strand, within H281DRAFT_04809 at 244.828 kb on + strand, within H281DRAFT_04809 at 244.829 kb on - strand, within H281DRAFT_04809 at 244.829 kb on - strand, within H281DRAFT_04809 at 244.829 kb on - strand, within H281DRAFT_04809 at 244.829 kb on - strand, within H281DRAFT_04809 at 244.829 kb on - strand, within H281DRAFT_04809 at 244.953 kb on + strand, within H281DRAFT_04809 at 244.954 kb on - strand, within H281DRAFT_04809 at 244.954 kb on - strand, within H281DRAFT_04809 at 244.954 kb on - strand, within H281DRAFT_04809 at 244.954 kb on - strand, within H281DRAFT_04809 at 244.954 kb on - strand, within H281DRAFT_04809 at 245.086 kb on + strand at 245.273 kb on + strand at 245.274 kb on - strand, within H281DRAFT_04810 at 245.274 kb on - strand, within H281DRAFT_04810 at 245.447 kb on + strand, within H281DRAFT_04810 at 245.447 kb on + strand, within H281DRAFT_04810 at 245.447 kb on + strand, within H281DRAFT_04810 at 245.448 kb on - strand, within H281DRAFT_04810 at 245.448 kb on - strand, within H281DRAFT_04810 at 245.451 kb on + strand, within H281DRAFT_04810 at 245.452 kb on - strand, within H281DRAFT_04810 at 245.452 kb on - strand, within H281DRAFT_04810 at 245.492 kb on + strand, within H281DRAFT_04810 at 245.493 kb on - strand, within H281DRAFT_04810 at 245.493 kb on - strand, within H281DRAFT_04810 at 245.536 kb on + strand, within H281DRAFT_04810 at 245.536 kb on + strand, within H281DRAFT_04810 at 245.537 kb on - strand, within H281DRAFT_04810 at 245.570 kb on - strand, within H281DRAFT_04810 at 245.589 kb on - strand, within H281DRAFT_04810 at 245.607 kb on + strand, within H281DRAFT_04810 at 245.607 kb on + strand, within H281DRAFT_04810 at 245.715 kb on - strand, within H281DRAFT_04810 at 245.737 kb on - strand, within H281DRAFT_04810 at 245.793 kb on + strand, within H281DRAFT_04810 at 246.099 kb on - strand, within H281DRAFT_04810 at 246.104 kb on + strand, within H281DRAFT_04810 at 246.112 kb on + strand, within H281DRAFT_04810 at 246.112 kb on + strand, within H281DRAFT_04810 at 246.113 kb on - strand, within H281DRAFT_04810 at 246.149 kb on + strand, within H281DRAFT_04810 at 246.174 kb on + strand, within H281DRAFT_04810 at 246.174 kb on + strand, within H281DRAFT_04810 at 246.174 kb on + strand, within H281DRAFT_04810 at 246.174 kb on + strand, within H281DRAFT_04810 at 246.180 kb on + strand, within H281DRAFT_04810 at 246.180 kb on + strand, within H281DRAFT_04810 at 246.181 kb on - strand, within H281DRAFT_04810 at 246.185 kb on + strand, within H281DRAFT_04810 at 246.186 kb on - strand, within H281DRAFT_04810 at 246.220 kb on + strand, within H281DRAFT_04810 at 246.220 kb on + strand, within H281DRAFT_04810 at 246.265 kb on + strand at 246.289 kb on - strand at 246.363 kb on + strand at 246.364 kb on - strand at 246.364 kb on - strand at 246.379 kb on - strand at 246.421 kb on + strand at 246.422 kb on - strand at 246.422 kb on - strand at 246.428 kb on + strand at 246.471 kb on + strand at 246.724 kb on - strand, within H281DRAFT_04811 at 246.725 kb on + strand, within H281DRAFT_04811 at 246.725 kb on + strand, within H281DRAFT_04811 at 246.725 kb on + strand, within H281DRAFT_04811 at 246.767 kb on + strand, within H281DRAFT_04811 at 246.773 kb on + strand, within H281DRAFT_04811 at 246.773 kb on + strand, within H281DRAFT_04811 at 246.927 kb on + strand, within H281DRAFT_04811 at 246.928 kb on - strand, within H281DRAFT_04811 at 246.928 kb on - strand, within H281DRAFT_04811 at 246.969 kb on - strand, within H281DRAFT_04811 at 247.204 kb on + strand, within H281DRAFT_04811 at 247.204 kb on + strand, within H281DRAFT_04811 at 247.225 kb on + strand, within H281DRAFT_04811 at 247.226 kb on - strand at 247.226 kb on - strand, within H281DRAFT_04811 at 247.226 kb on - strand, within H281DRAFT_04811 at 247.359 kb on + strand, within H281DRAFT_04811 at 247.359 kb on + strand, within H281DRAFT_04811 at 247.359 kb on + strand, within H281DRAFT_04811 at 247.360 kb on - strand, within H281DRAFT_04811 at 247.360 kb on - strand, within H281DRAFT_04811 at 247.361 kb on + strand, within H281DRAFT_04811
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 244,340 + +0.6 244,340 + -0.8 244,340 + +3.1 244,340 + -0.4 244,340 + +0.7 244,340 + +1.3 244,340 + -0.5 244,341 - -0.6 244,341 - +0.9 244,516 - +0.5 244,521 + +0.3 244,521 + -0.2 244,565 - +0.9 244,571 + +0.4 244,689 + H281DRAFT_04809 0.27 +0.3 244,689 + H281DRAFT_04809 0.27 -0.5 244,740 + H281DRAFT_04809 0.36 -0.1 244,740 + H281DRAFT_04809 0.36 +0.0 244,740 + H281DRAFT_04809 0.36 +1.3 244,740 + H281DRAFT_04809 0.36 -0.2 244,740 + H281DRAFT_04809 0.36 -0.1 244,741 - H281DRAFT_04809 0.36 +1.0 244,741 - H281DRAFT_04809 0.36 -1.2 244,741 - H281DRAFT_04809 0.36 -0.2 244,741 - H281DRAFT_04809 0.36 -0.1 244,741 - H281DRAFT_04809 0.36 +0.2 244,741 - H281DRAFT_04809 0.36 +0.7 244,741 - H281DRAFT_04809 0.36 +0.3 244,741 - H281DRAFT_04809 0.36 -0.5 244,742 + H281DRAFT_04809 0.36 -0.2 244,742 + H281DRAFT_04809 0.36 +1.0 244,742 + H281DRAFT_04809 0.36 +0.3 244,742 + H281DRAFT_04809 0.36 -0.4 244,743 - H281DRAFT_04809 0.36 -0.2 244,743 - H281DRAFT_04809 0.36 -0.1 244,743 - H281DRAFT_04809 0.36 +0.3 244,743 - -1.0 244,743 - H281DRAFT_04809 0.36 -0.0 244,819 + H281DRAFT_04809 0.49 +0.5 244,819 + H281DRAFT_04809 0.49 +0.1 244,820 - H281DRAFT_04809 0.49 +0.5 244,820 - H281DRAFT_04809 0.49 +0.0 244,820 - H281DRAFT_04809 0.49 +1.4 244,822 + H281DRAFT_04809 0.49 +0.3 244,823 - H281DRAFT_04809 0.49 -0.5 244,828 + H281DRAFT_04809 0.50 +0.4 244,828 + H281DRAFT_04809 0.50 +0.0 244,829 - H281DRAFT_04809 0.50 +1.6 244,829 - H281DRAFT_04809 0.50 -0.6 244,829 - H281DRAFT_04809 0.50 -0.6 244,829 - H281DRAFT_04809 0.50 +0.4 244,829 - H281DRAFT_04809 0.50 +0.5 244,953 + H281DRAFT_04809 0.70 +0.2 244,954 - H281DRAFT_04809 0.71 -0.5 244,954 - H281DRAFT_04809 0.71 +0.4 244,954 - H281DRAFT_04809 0.71 -0.1 244,954 - H281DRAFT_04809 0.71 -0.4 244,954 - H281DRAFT_04809 0.71 -0.1 245,086 + +0.4 245,273 + +0.6 245,274 - H281DRAFT_04810 0.10 -0.6 245,274 - H281DRAFT_04810 0.10 -2.0 245,447 + H281DRAFT_04810 0.24 +0.3 245,447 + H281DRAFT_04810 0.24 +0.6 245,447 + H281DRAFT_04810 0.24 +0.5 245,448 - H281DRAFT_04810 0.24 -0.5 245,448 - H281DRAFT_04810 0.24 -0.5 245,451 + H281DRAFT_04810 0.25 +2.0 245,452 - H281DRAFT_04810 0.25 -0.6 245,452 - H281DRAFT_04810 0.25 -0.2 245,492 + H281DRAFT_04810 0.28 +0.3 245,493 - H281DRAFT_04810 0.28 -0.0 245,493 - H281DRAFT_04810 0.28 -0.5 245,536 + H281DRAFT_04810 0.32 -0.1 245,536 + H281DRAFT_04810 0.32 +0.4 245,537 - H281DRAFT_04810 0.32 -0.2 245,570 - H281DRAFT_04810 0.34 -0.4 245,589 - H281DRAFT_04810 0.36 -0.9 245,607 + H281DRAFT_04810 0.38 -0.2 245,607 + H281DRAFT_04810 0.38 +0.8 245,715 - H281DRAFT_04810 0.46 -0.5 245,737 - H281DRAFT_04810 0.48 -0.1 245,793 + H281DRAFT_04810 0.53 -1.8 246,099 - H281DRAFT_04810 0.78 +0.3 246,104 + H281DRAFT_04810 0.79 +1.0 246,112 + H281DRAFT_04810 0.79 +0.5 246,112 + H281DRAFT_04810 0.79 -0.2 246,113 - H281DRAFT_04810 0.79 +0.7 246,149 + H281DRAFT_04810 0.82 +0.4 246,174 + H281DRAFT_04810 0.84 +0.3 246,174 + H281DRAFT_04810 0.84 -0.6 246,174 + H281DRAFT_04810 0.84 -0.6 246,174 + H281DRAFT_04810 0.84 -0.4 246,180 + H281DRAFT_04810 0.85 -1.0 246,180 + H281DRAFT_04810 0.85 -0.0 246,181 - H281DRAFT_04810 0.85 +0.1 246,185 + H281DRAFT_04810 0.85 -0.6 246,186 - H281DRAFT_04810 0.85 +0.1 246,220 + H281DRAFT_04810 0.88 -0.2 246,220 + H281DRAFT_04810 0.88 -0.5 246,265 + +0.3 246,289 - +0.1 246,363 + -1.4 246,364 - -0.5 246,364 - +0.8 246,379 - +1.3 246,421 + +0.1 246,422 - +0.8 246,422 - -0.5 246,428 + -0.7 246,471 + -0.4 246,724 - H281DRAFT_04811 0.11 -0.2 246,725 + H281DRAFT_04811 0.11 +0.6 246,725 + H281DRAFT_04811 0.11 -0.6 246,725 + H281DRAFT_04811 0.11 +1.7 246,767 + H281DRAFT_04811 0.16 -0.4 246,773 + H281DRAFT_04811 0.16 +0.1 246,773 + H281DRAFT_04811 0.16 -0.8 246,927 + H281DRAFT_04811 0.34 -1.3 246,928 - H281DRAFT_04811 0.34 -0.0 246,928 - H281DRAFT_04811 0.34 -0.2 246,969 - H281DRAFT_04811 0.39 -0.0 247,204 + H281DRAFT_04811 0.67 -0.3 247,204 + H281DRAFT_04811 0.67 +0.1 247,225 + H281DRAFT_04811 0.69 -1.2 247,226 - +3.5 247,226 - H281DRAFT_04811 0.69 -0.5 247,226 - H281DRAFT_04811 0.69 -0.2 247,359 + H281DRAFT_04811 0.85 -0.7 247,359 + H281DRAFT_04811 0.85 -0.2 247,359 + H281DRAFT_04811 0.85 -0.9 247,360 - H281DRAFT_04811 0.85 -0.3 247,360 - H281DRAFT_04811 0.85 -0.5 247,361 + H281DRAFT_04811 0.85 +1.4
Or see this region's nucleotide sequence