Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04595 and H281DRAFT_04596 are separated by 122 nucleotides H281DRAFT_04596 and H281DRAFT_04597 are separated by 121 nucleotides
H281DRAFT_04595: H281DRAFT_04595 - 3-hydroxyacyl-CoA dehydrogenase, at 326,615 to 328,180
_04595
H281DRAFT_04596: H281DRAFT_04596 - glutathione S-transferase, at 328,303 to 328,950
_04596
H281DRAFT_04597: H281DRAFT_04597 - 2-keto-4-methylthiobutyrate aminotransferase apoenzyme, at 329,072 to 330,244
_04597
Position (kb)
328
329 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 327.319 kb on - strand, within H281DRAFT_04595 at 327.324 kb on + strand, within H281DRAFT_04595 at 327.382 kb on + strand, within H281DRAFT_04595 at 327.383 kb on - strand, within H281DRAFT_04595 at 327.395 kb on - strand, within H281DRAFT_04595 at 327.411 kb on + strand, within H281DRAFT_04595 at 327.423 kb on + strand, within H281DRAFT_04595 at 327.424 kb on - strand, within H281DRAFT_04595 at 327.449 kb on + strand, within H281DRAFT_04595 at 327.665 kb on + strand, within H281DRAFT_04595 at 327.867 kb on - strand, within H281DRAFT_04595 at 327.867 kb on - strand, within H281DRAFT_04595 at 328.032 kb on + strand at 328.033 kb on - strand at 328.072 kb on - strand at 328.157 kb on + strand at 328.158 kb on - strand at 328.202 kb on + strand at 328.213 kb on - strand at 328.333 kb on - strand at 328.336 kb on + strand at 328.336 kb on + strand at 328.337 kb on - strand at 328.337 kb on - strand at 328.339 kb on + strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.397 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.495 kb on - strand, within H281DRAFT_04596 at 328.495 kb on - strand, within H281DRAFT_04596 at 328.504 kb on + strand, within H281DRAFT_04596 at 328.819 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.924 kb on + strand at 328.925 kb on - strand at 329.073 kb on + strand at 329.074 kb on - strand at 329.074 kb on - strand at 329.138 kb on + strand at 329.138 kb on + strand at 329.138 kb on + strand at 329.139 kb on - strand at 329.256 kb on + strand, within H281DRAFT_04597 at 329.274 kb on + strand, within H281DRAFT_04597 at 329.275 kb on - strand, within H281DRAFT_04597 at 329.391 kb on + strand, within H281DRAFT_04597 at 329.392 kb on - strand, within H281DRAFT_04597 at 329.392 kb on - strand, within H281DRAFT_04597 at 329.517 kb on + strand, within H281DRAFT_04597 at 329.517 kb on + strand, within H281DRAFT_04597 at 329.518 kb on - strand, within H281DRAFT_04597 at 329.518 kb on - strand, within H281DRAFT_04597 at 329.518 kb on - strand, within H281DRAFT_04597 at 329.518 kb on - strand, within H281DRAFT_04597 at 329.524 kb on + strand, within H281DRAFT_04597 at 329.525 kb on - strand, within H281DRAFT_04597 at 329.525 kb on - strand, within H281DRAFT_04597 at 329.616 kb on + strand, within H281DRAFT_04597 at 329.616 kb on + strand, within H281DRAFT_04597 at 329.617 kb on - strand, within H281DRAFT_04597 at 329.617 kb on - strand, within H281DRAFT_04597 at 329.617 kb on - strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.618 kb on + strand, within H281DRAFT_04597 at 329.619 kb on - strand, within H281DRAFT_04597 at 329.619 kb on - strand, within H281DRAFT_04597 at 329.619 kb on - strand, within H281DRAFT_04597 at 329.843 kb on + strand, within H281DRAFT_04597 at 329.843 kb on + strand, within H281DRAFT_04597 at 329.844 kb on - strand, within H281DRAFT_04597 at 329.844 kb on - strand, within H281DRAFT_04597 at 329.847 kb on + strand, within H281DRAFT_04597 at 329.847 kb on + strand, within H281DRAFT_04597 at 329.848 kb on - strand, within H281DRAFT_04597 at 329.848 kb on - strand, within H281DRAFT_04597 at 329.848 kb on - strand, within H281DRAFT_04597 at 329.856 kb on + strand, within H281DRAFT_04597 at 329.856 kb on + strand, within H281DRAFT_04597 at 329.877 kb on - strand, within H281DRAFT_04597 at 329.877 kb on - strand, within H281DRAFT_04597
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 327,319 - H281DRAFT_04595 0.45 +0.0 327,324 + H281DRAFT_04595 0.45 +0.6 327,382 + H281DRAFT_04595 0.49 +0.7 327,383 - H281DRAFT_04595 0.49 +0.5 327,395 - H281DRAFT_04595 0.50 +0.1 327,411 + H281DRAFT_04595 0.51 +0.2 327,423 + H281DRAFT_04595 0.52 -0.2 327,424 - H281DRAFT_04595 0.52 +0.2 327,449 + H281DRAFT_04595 0.53 +1.1 327,665 + H281DRAFT_04595 0.67 +0.4 327,867 - H281DRAFT_04595 0.80 -0.2 327,867 - H281DRAFT_04595 0.80 -1.1 328,032 + +0.3 328,033 - -0.3 328,072 - +0.1 328,157 + +0.8 328,158 - +0.5 328,202 + +1.1 328,213 - -2.4 328,333 - -0.5 328,336 + +0.2 328,336 + -0.6 328,337 - -1.6 328,337 - -0.8 328,339 + -0.2 328,340 - -2.3 328,340 - -2.4 328,340 - -5.3 328,397 + H281DRAFT_04596 0.15 -0.6 328,494 + H281DRAFT_04596 0.29 -0.0 328,494 + H281DRAFT_04596 0.29 -0.3 328,494 + H281DRAFT_04596 0.29 -0.1 328,494 + H281DRAFT_04596 0.29 -0.1 328,495 - H281DRAFT_04596 0.30 -2.7 328,495 - H281DRAFT_04596 0.30 -2.3 328,504 + H281DRAFT_04596 0.31 +0.2 328,819 + H281DRAFT_04596 0.80 +0.5 328,864 + H281DRAFT_04596 0.87 -0.4 328,864 + H281DRAFT_04596 0.87 -1.4 328,864 + H281DRAFT_04596 0.87 +1.9 328,864 + H281DRAFT_04596 0.87 -0.3 328,924 + +0.4 328,925 - -0.4 329,073 + -1.1 329,074 - -0.0 329,074 - +0.2 329,138 + -0.5 329,138 + +0.3 329,138 + -0.2 329,139 - +0.0 329,256 + H281DRAFT_04597 0.16 -0.2 329,274 + H281DRAFT_04597 0.17 +0.4 329,275 - H281DRAFT_04597 0.17 +0.1 329,391 + H281DRAFT_04597 0.27 -0.0 329,392 - H281DRAFT_04597 0.27 +0.6 329,392 - H281DRAFT_04597 0.27 -0.1 329,517 + H281DRAFT_04597 0.38 -0.1 329,517 + H281DRAFT_04597 0.38 +0.5 329,518 - H281DRAFT_04597 0.38 +0.1 329,518 - H281DRAFT_04597 0.38 +0.1 329,518 - H281DRAFT_04597 0.38 -0.2 329,518 - H281DRAFT_04597 0.38 -0.8 329,524 + H281DRAFT_04597 0.39 +0.5 329,525 - H281DRAFT_04597 0.39 -0.1 329,525 - H281DRAFT_04597 0.39 +0.7 329,616 + H281DRAFT_04597 0.46 +0.6 329,616 + H281DRAFT_04597 0.46 -0.9 329,617 - H281DRAFT_04597 0.46 -1.2 329,617 - H281DRAFT_04597 0.46 -0.6 329,617 - H281DRAFT_04597 0.46 +0.0 329,618 + H281DRAFT_04597 0.47 +0.9 329,618 + H281DRAFT_04597 0.47 +0.1 329,618 + H281DRAFT_04597 0.47 -0.2 329,618 + H281DRAFT_04597 0.47 +0.2 329,618 + H281DRAFT_04597 0.47 -0.2 329,618 + H281DRAFT_04597 0.47 -0.4 329,618 + H281DRAFT_04597 0.47 +0.2 329,619 - H281DRAFT_04597 0.47 -0.4 329,619 - H281DRAFT_04597 0.47 -0.0 329,619 - H281DRAFT_04597 0.47 -1.2 329,843 + H281DRAFT_04597 0.66 +0.4 329,843 + H281DRAFT_04597 0.66 +0.7 329,844 - H281DRAFT_04597 0.66 +0.2 329,844 - H281DRAFT_04597 0.66 -0.0 329,847 + H281DRAFT_04597 0.66 -0.3 329,847 + H281DRAFT_04597 0.66 -0.1 329,848 - H281DRAFT_04597 0.66 +0.0 329,848 - H281DRAFT_04597 0.66 -0.8 329,848 - H281DRAFT_04597 0.66 +1.3 329,856 + H281DRAFT_04597 0.67 +0.4 329,856 + H281DRAFT_04597 0.67 -0.0 329,877 - H281DRAFT_04597 0.69 -0.9 329,877 - H281DRAFT_04597 0.69 +0.3
Or see this region's nucleotide sequence