Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04582 and H281DRAFT_04583 are separated by 317 nucleotides H281DRAFT_04583 and H281DRAFT_04584 are separated by 45 nucleotides H281DRAFT_04584 and H281DRAFT_04585 are separated by 260 nucleotides H281DRAFT_04585 and H281DRAFT_04586 are separated by 48 nucleotides
H281DRAFT_04582: H281DRAFT_04582 - ADP-ribose pyrophosphatase, at 315,839 to 316,429
_04582
H281DRAFT_04583: H281DRAFT_04583 - hypothetical protein, at 316,747 to 316,992
_04583
H281DRAFT_04584: H281DRAFT_04584 - transcriptional regulator, AlpA family, at 317,038 to 317,562
_04584
H281DRAFT_04585: H281DRAFT_04585 - hypothetical protein, at 317,823 to 318,248
_04585
H281DRAFT_04586: H281DRAFT_04586 - hypothetical protein, at 318,297 to 319,493
_04586
Position (kb)
317
318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 316.116 kb on + strand, within H281DRAFT_04582 at 316.156 kb on + strand, within H281DRAFT_04582 at 316.170 kb on - strand, within H281DRAFT_04582 at 316.205 kb on + strand, within H281DRAFT_04582 at 316.359 kb on + strand, within H281DRAFT_04582 at 316.360 kb on - strand, within H281DRAFT_04582 at 316.466 kb on + strand at 316.466 kb on + strand at 316.466 kb on + strand at 316.467 kb on - strand at 316.467 kb on - strand at 316.554 kb on + strand at 316.572 kb on - strand at 316.606 kb on - strand at 316.611 kb on + strand at 316.612 kb on - strand at 316.612 kb on - strand at 316.614 kb on + strand at 316.615 kb on - strand at 316.691 kb on + strand at 316.732 kb on - strand at 316.778 kb on + strand, within H281DRAFT_04583 at 316.778 kb on + strand, within H281DRAFT_04583 at 316.779 kb on - strand, within H281DRAFT_04583 at 316.779 kb on - strand, within H281DRAFT_04583 at 316.858 kb on + strand, within H281DRAFT_04583 at 316.858 kb on + strand at 316.858 kb on + strand, within H281DRAFT_04583 at 316.859 kb on - strand, within H281DRAFT_04583 at 316.859 kb on - strand, within H281DRAFT_04583 at 316.868 kb on - strand, within H281DRAFT_04583 at 316.870 kb on - strand, within H281DRAFT_04583 at 316.870 kb on - strand, within H281DRAFT_04583 at 317.032 kb on + strand at 317.032 kb on + strand at 317.033 kb on - strand at 317.045 kb on - strand at 317.232 kb on + strand, within H281DRAFT_04584 at 317.443 kb on + strand, within H281DRAFT_04584 at 317.462 kb on + strand at 317.630 kb on + strand at 317.630 kb on + strand at 317.631 kb on - strand at 317.637 kb on + strand at 317.638 kb on - strand at 317.638 kb on - strand at 317.638 kb on - strand at 317.695 kb on + strand at 317.839 kb on + strand at 317.839 kb on + strand at 317.840 kb on - strand at 318.062 kb on - strand, within H281DRAFT_04585 at 318.144 kb on - strand, within H281DRAFT_04585 at 318.246 kb on + strand at 318.292 kb on + strand at 318.292 kb on + strand at 318.292 kb on + strand at 318.293 kb on - strand at 318.293 kb on - strand at 318.293 kb on - strand at 318.293 kb on - strand at 318.296 kb on + strand at 318.296 kb on + strand at 318.296 kb on + strand at 318.297 kb on - strand at 318.309 kb on + strand at 318.309 kb on + strand at 318.391 kb on + strand at 318.392 kb on - strand at 318.401 kb on + strand at 318.401 kb on + strand at 318.402 kb on - strand at 318.487 kb on - strand, within H281DRAFT_04586 at 318.521 kb on + strand, within H281DRAFT_04586 at 318.521 kb on + strand, within H281DRAFT_04586 at 318.521 kb on + strand, within H281DRAFT_04586 at 318.521 kb on + strand, within H281DRAFT_04586 at 318.521 kb on + strand, within H281DRAFT_04586 at 318.522 kb on - strand, within H281DRAFT_04586 at 318.562 kb on + strand, within H281DRAFT_04586
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 316,116 + H281DRAFT_04582 0.47 -0.3 316,156 + H281DRAFT_04582 0.54 +1.2 316,170 - H281DRAFT_04582 0.56 +0.4 316,205 + H281DRAFT_04582 0.62 +0.6 316,359 + H281DRAFT_04582 0.88 -0.4 316,360 - H281DRAFT_04582 0.88 -0.1 316,466 + +0.3 316,466 + +0.1 316,466 + +2.5 316,467 - -0.4 316,467 - +0.5 316,554 + +0.0 316,572 - +0.6 316,606 - +0.6 316,611 + +1.2 316,612 - -0.4 316,612 - -0.1 316,614 + +1.3 316,615 - -0.5 316,691 + +0.4 316,732 - -0.6 316,778 + H281DRAFT_04583 0.13 -1.2 316,778 + H281DRAFT_04583 0.13 -0.2 316,779 - H281DRAFT_04583 0.13 -0.0 316,779 - H281DRAFT_04583 0.13 -0.5 316,858 + H281DRAFT_04583 0.45 +1.0 316,858 + +1.9 316,858 + H281DRAFT_04583 0.45 +0.4 316,859 - H281DRAFT_04583 0.46 -0.3 316,859 - H281DRAFT_04583 0.46 +0.2 316,868 - H281DRAFT_04583 0.49 -0.1 316,870 - H281DRAFT_04583 0.50 -0.0 316,870 - H281DRAFT_04583 0.50 -0.5 317,032 + +0.6 317,032 + +0.0 317,033 - -0.1 317,045 - -0.5 317,232 + H281DRAFT_04584 0.37 -0.7 317,443 + H281DRAFT_04584 0.77 +0.6 317,462 + +0.9 317,630 + +0.1 317,630 + -0.8 317,631 - -0.1 317,637 + +0.2 317,638 - +0.4 317,638 - +0.7 317,638 - -1.3 317,695 + +0.0 317,839 + +1.9 317,839 + +0.3 317,840 - -0.1 318,062 - H281DRAFT_04585 0.56 -1.0 318,144 - H281DRAFT_04585 0.75 -0.8 318,246 + +0.5 318,292 + +0.8 318,292 + +0.5 318,292 + +0.5 318,293 - +0.9 318,293 - +1.3 318,293 - -3.5 318,293 - -0.2 318,296 + +0.1 318,296 + +0.6 318,296 + -0.3 318,297 - +1.1 318,309 + -0.0 318,309 + +0.5 318,391 + +0.7 318,392 - +0.0 318,401 + +0.3 318,401 + -0.5 318,402 - +0.0 318,487 - H281DRAFT_04586 0.16 +1.4 318,521 + H281DRAFT_04586 0.19 -0.1 318,521 + H281DRAFT_04586 0.19 -0.1 318,521 + H281DRAFT_04586 0.19 +0.2 318,521 + H281DRAFT_04586 0.19 +0.3 318,521 + H281DRAFT_04586 0.19 -1.1 318,522 - H281DRAFT_04586 0.19 +0.7 318,562 + H281DRAFT_04586 0.22 +0.5
Or see this region's nucleotide sequence