Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04481 and H281DRAFT_04482 overlap by 4 nucleotides H281DRAFT_04482 and H281DRAFT_04483 are separated by 167 nucleotides H281DRAFT_04483 and H281DRAFT_04484 are separated by 37 nucleotides H281DRAFT_04484 and H281DRAFT_04485 are separated by 240 nucleotides
H281DRAFT_04481: H281DRAFT_04481 - Uncharacterized membrane protein, at 212,651 to 213,058
_04481
H281DRAFT_04482: H281DRAFT_04482 - NAD(P)H dehydrogenase (quinone), at 213,055 to 213,657
_04482
H281DRAFT_04483: H281DRAFT_04483 - tRNA-processing RNAse BN, at 213,825 to 215,135
_04483
H281DRAFT_04484: H281DRAFT_04484 - hypothetical protein, at 215,173 to 215,493
_04484
H281DRAFT_04485: H281DRAFT_04485 - Pimeloyl-ACP methyl ester carboxylesterase, at 215,734 to 216,567
_04485
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -1
0
1
2
3 at 212.835 kb on + strand at 212.871 kb on + strand, within H281DRAFT_04481 at 212.872 kb on - strand, within H281DRAFT_04481 at 213.227 kb on + strand, within H281DRAFT_04482 at 213.392 kb on + strand at 213.392 kb on + strand, within H281DRAFT_04482 at 213.393 kb on - strand, within H281DRAFT_04482 at 213.632 kb on + strand at 213.633 kb on - strand at 213.633 kb on - strand at 213.635 kb on + strand at 213.635 kb on + strand at 213.636 kb on - strand at 213.658 kb on - strand at 213.678 kb on + strand at 213.679 kb on - strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.681 kb on + strand at 213.682 kb on - strand at 213.683 kb on + strand at 213.778 kb on + strand at 213.903 kb on - strand at 214.081 kb on - strand, within H281DRAFT_04483 at 214.300 kb on - strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.413 kb on + strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.420 kb on - strand, within H281DRAFT_04483 at 214.512 kb on + strand, within H281DRAFT_04483 at 214.516 kb on - strand, within H281DRAFT_04483 at 214.537 kb on - strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.590 kb on + strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.591 kb on - strand, within H281DRAFT_04483 at 214.755 kb on + strand, within H281DRAFT_04483 at 214.923 kb on + strand, within H281DRAFT_04483 at 215.133 kb on + strand at 215.134 kb on - strand at 215.175 kb on - strand at 215.191 kb on + strand at 215.232 kb on + strand, within H281DRAFT_04484 at 215.232 kb on + strand, within H281DRAFT_04484 at 215.235 kb on - strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.435 kb on + strand, within H281DRAFT_04484 at 215.436 kb on - strand, within H281DRAFT_04484 at 215.444 kb on + strand, within H281DRAFT_04484 at 215.484 kb on + strand at 215.838 kb on + strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.839 kb on - strand, within H281DRAFT_04485 at 215.976 kb on - strand, within H281DRAFT_04485 at 215.979 kb on + strand, within H281DRAFT_04485 at 215.979 kb on + strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485 at 215.980 kb on - strand, within H281DRAFT_04485
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 212,835 + -0.1 212,871 + H281DRAFT_04481 0.54 -0.8 212,872 - H281DRAFT_04481 0.54 -0.3 213,227 + H281DRAFT_04482 0.29 -1.0 213,392 + +0.9 213,392 + H281DRAFT_04482 0.56 -0.5 213,393 - H281DRAFT_04482 0.56 +0.0 213,632 + -0.4 213,633 - -0.2 213,633 - +1.1 213,635 + -1.2 213,635 + -0.0 213,636 - +0.1 213,658 - +0.2 213,678 + -0.5 213,679 - -0.2 213,681 + +0.2 213,681 + +0.4 213,681 + +0.9 213,682 - +0.4 213,683 + -0.0 213,778 + -1.0 213,903 - +0.8 214,081 - H281DRAFT_04483 0.20 +0.5 214,300 - H281DRAFT_04483 0.36 +0.6 214,413 + H281DRAFT_04483 0.45 +0.1 214,413 + H281DRAFT_04483 0.45 -0.3 214,420 - H281DRAFT_04483 0.45 +0.0 214,420 - H281DRAFT_04483 0.45 +0.3 214,512 + H281DRAFT_04483 0.52 -0.0 214,516 - H281DRAFT_04483 0.53 -1.4 214,537 - H281DRAFT_04483 0.54 -0.0 214,590 + H281DRAFT_04483 0.58 +0.2 214,590 + H281DRAFT_04483 0.58 -0.4 214,591 - H281DRAFT_04483 0.58 +1.2 214,591 - H281DRAFT_04483 0.58 +1.0 214,591 - H281DRAFT_04483 0.58 -0.3 214,755 + H281DRAFT_04483 0.71 -0.7 214,923 + H281DRAFT_04483 0.84 -0.4 215,133 + +0.4 215,134 - -0.3 215,175 - -0.3 215,191 + -0.7 215,232 + H281DRAFT_04484 0.18 +0.9 215,232 + H281DRAFT_04484 0.18 -0.4 215,235 - H281DRAFT_04484 0.19 +1.5 215,435 + H281DRAFT_04484 0.82 +0.2 215,435 + H281DRAFT_04484 0.82 -0.1 215,435 + H281DRAFT_04484 0.82 +0.2 215,436 - H281DRAFT_04484 0.82 +3.2 215,444 + H281DRAFT_04484 0.84 +0.1 215,484 + -0.1 215,838 + H281DRAFT_04485 0.12 -0.1 215,839 - H281DRAFT_04485 0.13 +0.5 215,839 - H281DRAFT_04485 0.13 -0.2 215,839 - H281DRAFT_04485 0.13 +0.3 215,839 - H281DRAFT_04485 0.13 -1.2 215,976 - H281DRAFT_04485 0.29 +0.9 215,979 + H281DRAFT_04485 0.29 -0.3 215,979 + H281DRAFT_04485 0.29 -0.3 215,980 - H281DRAFT_04485 0.29 -0.1 215,980 - H281DRAFT_04485 0.29 +0.0 215,980 - H281DRAFT_04485 0.29 +0.1
Or see this region's nucleotide sequence