Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04266 and H281DRAFT_04267 are separated by 122 nucleotides H281DRAFT_04267 and H281DRAFT_04268 overlap by 4 nucleotides H281DRAFT_04268 and H281DRAFT_04269 overlap by 1 nucleotides H281DRAFT_04269 and H281DRAFT_04270 are separated by 82 nucleotides
H281DRAFT_04266: H281DRAFT_04266 - glutamine amidotransferase, at 384,311 to 385,246
_04266
H281DRAFT_04267: H281DRAFT_04267 - L-glutamate ABC transporter ATP-binding protein /L-aspartate ABC transporter ATP-binding protein, at 385,369 to 386,094
_04267
H281DRAFT_04268: H281DRAFT_04268 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,091 to 386,768
_04268
H281DRAFT_04269: H281DRAFT_04269 - L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein, at 386,768 to 387,508
_04269
H281DRAFT_04270: H281DRAFT_04270 - L-glutamate-binding protein /L-aspartate-binding protein, at 387,591 to 388,484
_04270
Position (kb)
386
387 Strain fitness (log2 ratio)
-1
0
1
2
3 at 385.288 kb on - strand at 386.048 kb on - strand at 386.086 kb on - strand at 386.086 kb on - strand at 386.086 kb on - strand at 386.175 kb on + strand, within H281DRAFT_04268 at 386.175 kb on + strand, within H281DRAFT_04268 at 386.251 kb on + strand, within H281DRAFT_04268 at 386.252 kb on - strand, within H281DRAFT_04268 at 386.264 kb on + strand, within H281DRAFT_04268 at 386.265 kb on - strand, within H281DRAFT_04268 at 386.265 kb on - strand, within H281DRAFT_04268 at 386.356 kb on + strand, within H281DRAFT_04268 at 386.357 kb on - strand, within H281DRAFT_04268 at 386.357 kb on - strand, within H281DRAFT_04268 at 386.435 kb on - strand, within H281DRAFT_04268 at 386.435 kb on - strand, within H281DRAFT_04268 at 386.438 kb on - strand, within H281DRAFT_04268 at 386.438 kb on - strand, within H281DRAFT_04268 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.593 kb on + strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.594 kb on - strand, within H281DRAFT_04268 at 386.782 kb on + strand at 386.784 kb on + strand at 386.853 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.883 kb on + strand, within H281DRAFT_04269 at 386.884 kb on - strand, within H281DRAFT_04269 at 386.899 kb on - strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.974 kb on + strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 386.975 kb on - strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.036 kb on + strand, within H281DRAFT_04269 at 387.243 kb on - strand, within H281DRAFT_04269 at 387.252 kb on + strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.253 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.304 kb on - strand, within H281DRAFT_04269 at 387.444 kb on + strand at 387.444 kb on + strand at 387.445 kb on - strand at 387.445 kb on - strand at 387.501 kb on + strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.502 kb on - strand at 387.585 kb on + strand at 387.585 kb on + strand at 387.586 kb on - strand at 387.586 kb on - strand at 387.592 kb on + strand at 387.704 kb on - strand, within H281DRAFT_04270 at 387.704 kb on - strand, within H281DRAFT_04270
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 385,288 - -0.5 386,048 - +1.0 386,086 - +0.7 386,086 - +1.7 386,086 - +1.8 386,175 + H281DRAFT_04268 0.12 +0.9 386,175 + H281DRAFT_04268 0.12 +0.8 386,251 + H281DRAFT_04268 0.24 +0.3 386,252 - H281DRAFT_04268 0.24 +1.3 386,264 + H281DRAFT_04268 0.26 +0.9 386,265 - H281DRAFT_04268 0.26 +0.5 386,265 - H281DRAFT_04268 0.26 +1.4 386,356 + H281DRAFT_04268 0.39 +1.5 386,357 - H281DRAFT_04268 0.39 +0.9 386,357 - H281DRAFT_04268 0.39 +0.8 386,435 - H281DRAFT_04268 0.51 +2.9 386,435 - H281DRAFT_04268 0.51 +1.2 386,438 - H281DRAFT_04268 0.51 +1.9 386,438 - H281DRAFT_04268 0.51 +1.1 386,593 + H281DRAFT_04268 0.74 +1.3 386,593 + H281DRAFT_04268 0.74 +0.9 386,594 - H281DRAFT_04268 0.74 +1.4 386,594 - H281DRAFT_04268 0.74 +1.3 386,782 + +1.6 386,784 + +0.9 386,853 + H281DRAFT_04269 0.11 +0.8 386,883 + H281DRAFT_04269 0.16 +0.9 386,883 + H281DRAFT_04269 0.16 +1.6 386,884 - H281DRAFT_04269 0.16 +1.7 386,899 - H281DRAFT_04269 0.18 +1.1 386,974 + H281DRAFT_04269 0.28 +0.3 386,974 + H281DRAFT_04269 0.28 +2.5 386,974 + H281DRAFT_04269 0.28 +0.8 386,975 - H281DRAFT_04269 0.28 +1.0 386,975 - H281DRAFT_04269 0.28 +2.0 387,036 + H281DRAFT_04269 0.36 +0.5 387,036 + H281DRAFT_04269 0.36 +1.7 387,243 - H281DRAFT_04269 0.64 +2.1 387,252 + H281DRAFT_04269 0.65 -0.2 387,253 - H281DRAFT_04269 0.65 +1.9 387,253 - H281DRAFT_04269 0.65 +1.2 387,304 - H281DRAFT_04269 0.72 +1.2 387,304 - H281DRAFT_04269 0.72 +1.2 387,444 + +1.1 387,444 + -0.1 387,445 - +1.3 387,445 - +1.0 387,501 + +0.9 387,502 - +1.6 387,502 - +1.8 387,502 - +1.4 387,585 + +1.5 387,585 + +1.4 387,586 - +1.2 387,586 - +0.8 387,592 + +0.9 387,704 - H281DRAFT_04270 0.13 +1.7 387,704 - H281DRAFT_04270 0.13 +1.4
Or see this region's nucleotide sequence