Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04201 and H281DRAFT_04202 are separated by 99 nucleotides H281DRAFT_04202 and H281DRAFT_04203 are separated by 98 nucleotides H281DRAFT_04203 and H281DRAFT_04204 are separated by 145 nucleotides
H281DRAFT_04201: H281DRAFT_04201 - O-antigen ligase, at 320,233 to 322,050
_04201
H281DRAFT_04202: H281DRAFT_04202 - type IV pilus assembly protein PilA, at 322,150 to 322,773
_04202
H281DRAFT_04203: H281DRAFT_04203 - integral membrane protein, YjbE family, at 322,872 to 323,582
_04203
H281DRAFT_04204: H281DRAFT_04204 - succinyl-CoA synthetase (ADP-forming) alpha subunit, at 323,728 to 324,609
_04204
Position (kb)
322
323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 321.556 kb on + strand, within H281DRAFT_04201 at 321.936 kb on + strand at 321.936 kb on + strand at 321.936 kb on + strand at 321.937 kb on - strand at 321.937 kb on - strand at 321.937 kb on - strand at 321.937 kb on - strand at 322.052 kb on - strand at 322.054 kb on - strand at 322.152 kb on - strand at 322.152 kb on - strand at 322.152 kb on - strand at 322.191 kb on + strand at 322.192 kb on - strand at 322.192 kb on - strand at 322.351 kb on + strand, within H281DRAFT_04202 at 322.352 kb on - strand, within H281DRAFT_04202 at 322.599 kb on - strand, within H281DRAFT_04202 at 322.689 kb on + strand, within H281DRAFT_04202 at 322.755 kb on - strand at 322.918 kb on + strand at 323.021 kb on + strand, within H281DRAFT_04203 at 323.021 kb on + strand, within H281DRAFT_04203 at 323.021 kb on + strand, within H281DRAFT_04203 at 323.022 kb on - strand, within H281DRAFT_04203 at 323.022 kb on - strand, within H281DRAFT_04203 at 323.063 kb on + strand, within H281DRAFT_04203 at 323.404 kb on - strand, within H281DRAFT_04203 at 323.526 kb on + strand at 323.526 kb on + strand at 323.526 kb on + strand at 323.526 kb on + strand at 323.527 kb on - strand at 323.527 kb on - strand at 323.595 kb on - strand at 323.685 kb on - strand at 323.690 kb on + strand at 323.708 kb on - strand at 323.729 kb on + strand at 323.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 321,556 + H281DRAFT_04201 0.73 +2.3 321,936 + -0.3 321,936 + -0.6 321,936 + -0.0 321,937 - +0.4 321,937 - +0.7 321,937 - +0.6 321,937 - +0.7 322,052 - +0.4 322,054 - +0.5 322,152 - +1.4 322,152 - -0.0 322,152 - +1.0 322,191 + -0.2 322,192 - -1.2 322,192 - +0.9 322,351 + H281DRAFT_04202 0.32 -0.8 322,352 - H281DRAFT_04202 0.32 -0.7 322,599 - H281DRAFT_04202 0.72 -1.7 322,689 + H281DRAFT_04202 0.86 -0.5 322,755 - +0.4 322,918 + -0.2 323,021 + H281DRAFT_04203 0.21 -0.6 323,021 + H281DRAFT_04203 0.21 -1.3 323,021 + H281DRAFT_04203 0.21 +0.1 323,022 - H281DRAFT_04203 0.21 -0.4 323,022 - H281DRAFT_04203 0.21 +0.2 323,063 + H281DRAFT_04203 0.27 -0.4 323,404 - H281DRAFT_04203 0.75 +0.3 323,526 + -1.0 323,526 + -0.5 323,526 + +1.5 323,526 + -2.3 323,527 - +0.2 323,527 - +0.4 323,595 - +0.5 323,685 - +0.7 323,690 + +0.5 323,708 - +1.6 323,729 + -1.5 323,729 + +1.3
Or see this region's nucleotide sequence