Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04190 and H281DRAFT_04191 are separated by 266 nucleotides H281DRAFT_04191 and H281DRAFT_04192 overlap by 4 nucleotides H281DRAFT_04192 and H281DRAFT_04193 are separated by 283 nucleotides
H281DRAFT_04190: H281DRAFT_04190 - phosphoglycerate kinase, at 309,950 to 311,143
_04190
H281DRAFT_04191: H281DRAFT_04191 - Branched-chain amino acid transport protein, at 311,410 to 311,733
_04191
H281DRAFT_04192: H281DRAFT_04192 - Predicted branched-chain amino acid permease (azaleucine resistance), at 311,730 to 312,476
_04192
H281DRAFT_04193: H281DRAFT_04193 - branched chain amino acid aminotransferase apoenzyme, at 312,760 to 313,683
_04193
Position (kb)
311
312
313 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 311.313 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.412 kb on - strand at 311.513 kb on + strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.564 kb on - strand, within H281DRAFT_04191 at 311.766 kb on + strand at 312.064 kb on + strand, within H281DRAFT_04192 at 312.064 kb on + strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.108 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192 at 312.310 kb on + strand, within H281DRAFT_04192 at 312.419 kb on - strand at 312.456 kb on - strand at 312.456 kb on - strand at 312.565 kb on + strand at 312.565 kb on + strand at 312.565 kb on + strand at 312.565 kb on + strand at 312.565 kb on + strand at 312.565 kb on + strand at 312.566 kb on - strand at 312.566 kb on - strand at 312.631 kb on - strand at 312.675 kb on + strand at 312.734 kb on + strand at 312.759 kb on + strand at 312.759 kb on + strand at 312.759 kb on + strand at 312.759 kb on + strand at 312.760 kb on - strand at 313.150 kb on + strand, within H281DRAFT_04193 at 313.300 kb on + strand, within H281DRAFT_04193 at 313.333 kb on + strand, within H281DRAFT_04193 at 313.333 kb on + strand, within H281DRAFT_04193 at 313.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 311,313 + +1.0 311,411 + -0.5 311,411 + +0.0 311,411 + +1.0 311,411 + +0.0 311,412 - +0.2 311,513 + H281DRAFT_04191 0.32 -0.4 311,514 - H281DRAFT_04191 0.32 -2.9 311,514 - H281DRAFT_04191 0.32 -1.5 311,514 - H281DRAFT_04191 0.32 -1.6 311,514 - H281DRAFT_04191 0.32 +1.0 311,563 + H281DRAFT_04191 0.47 -2.4 311,563 + H281DRAFT_04191 0.47 -1.0 311,564 - H281DRAFT_04191 0.48 -1.5 311,766 + -1.6 312,064 + H281DRAFT_04192 0.45 -1.6 312,064 + H281DRAFT_04192 0.45 -0.6 312,065 - H281DRAFT_04192 0.45 -0.1 312,065 - H281DRAFT_04192 0.45 -1.4 312,065 - H281DRAFT_04192 0.45 +0.6 312,065 - H281DRAFT_04192 0.45 -2.5 312,108 + H281DRAFT_04192 0.51 -1.4 312,121 + H281DRAFT_04192 0.52 -1.4 312,121 + H281DRAFT_04192 0.52 -2.0 312,121 + H281DRAFT_04192 0.52 -1.0 312,121 + H281DRAFT_04192 0.52 -0.8 312,121 + H281DRAFT_04192 0.52 -1.7 312,122 - H281DRAFT_04192 0.52 -0.4 312,122 - H281DRAFT_04192 0.52 -1.0 312,122 - H281DRAFT_04192 0.52 -2.3 312,122 - H281DRAFT_04192 0.52 -0.7 312,122 - H281DRAFT_04192 0.52 -1.6 312,143 - H281DRAFT_04192 0.55 -1.5 312,143 - H281DRAFT_04192 0.55 -1.1 312,310 + H281DRAFT_04192 0.78 +0.8 312,419 - -1.1 312,456 - -0.9 312,456 - -0.7 312,565 + -2.0 312,565 + -0.0 312,565 + -0.4 312,565 + -1.1 312,565 + -0.6 312,565 + -0.5 312,566 - +0.4 312,566 - +0.5 312,631 - +0.5 312,675 + +0.1 312,734 + -0.3 312,759 + +0.9 312,759 + +0.3 312,759 + +0.2 312,759 + -0.9 312,760 - +1.1 313,150 + H281DRAFT_04193 0.42 +0.0 313,300 + H281DRAFT_04193 0.58 +0.2 313,333 + H281DRAFT_04193 0.62 -0.5 313,333 + H281DRAFT_04193 0.62 +0.6 313,387 + +1.0
Or see this region's nucleotide sequence