Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04189 and H281DRAFT_04190 are separated by 505 nucleotides H281DRAFT_04190 and H281DRAFT_04191 are separated by 266 nucleotides H281DRAFT_04191 and H281DRAFT_04192 overlap by 4 nucleotides
H281DRAFT_04189: H281DRAFT_04189 - pyruvate kinase, at 308,008 to 309,444
_04189
H281DRAFT_04190: H281DRAFT_04190 - phosphoglycerate kinase, at 309,950 to 311,143
_04190
H281DRAFT_04191: H281DRAFT_04191 - Branched-chain amino acid transport protein, at 311,410 to 311,733
_04191
H281DRAFT_04192: H281DRAFT_04192 - Predicted branched-chain amino acid permease (azaleucine resistance), at 311,730 to 312,476
_04192
Position (kb) 309
310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 309.952 kb on - strand at 309.969 kb on - strand at 310.036 kb on - strand at 310.036 kb on - strand at 311.313 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.412 kb on - strand at 311.513 kb on + strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.564 kb on - strand, within H281DRAFT_04191 at 311.766 kb on + strand at 312.064 kb on + strand, within H281DRAFT_04192 at 312.064 kb on + strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.108 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 309,952 - -1.3 309,969 - +1.3 310,036 - +1.0 310,036 - +1.0 311,313 + +1.0 311,411 + -0.5 311,411 + +0.0 311,411 + +1.0 311,411 + +0.0 311,412 - +0.2 311,513 + H281DRAFT_04191 0.32 -0.4 311,514 - H281DRAFT_04191 0.32 -2.9 311,514 - H281DRAFT_04191 0.32 -1.5 311,514 - H281DRAFT_04191 0.32 -1.6 311,514 - H281DRAFT_04191 0.32 +1.0 311,563 + H281DRAFT_04191 0.47 -2.4 311,563 + H281DRAFT_04191 0.47 -1.0 311,564 - H281DRAFT_04191 0.48 -1.5 311,766 + -1.6 312,064 + H281DRAFT_04192 0.45 -1.6 312,064 + H281DRAFT_04192 0.45 -0.6 312,065 - H281DRAFT_04192 0.45 -0.1 312,065 - H281DRAFT_04192 0.45 -1.4 312,065 - H281DRAFT_04192 0.45 +0.6 312,065 - H281DRAFT_04192 0.45 -2.5 312,108 + H281DRAFT_04192 0.51 -1.4 312,121 + H281DRAFT_04192 0.52 -1.4 312,121 + H281DRAFT_04192 0.52 -2.0 312,121 + H281DRAFT_04192 0.52 -1.0 312,121 + H281DRAFT_04192 0.52 -0.8 312,121 + H281DRAFT_04192 0.52 -1.7 312,122 - H281DRAFT_04192 0.52 -0.4 312,122 - H281DRAFT_04192 0.52 -1.0 312,122 - H281DRAFT_04192 0.52 -2.3 312,122 - H281DRAFT_04192 0.52 -0.7 312,122 - H281DRAFT_04192 0.52 -1.6 312,143 - H281DRAFT_04192 0.55 -1.5 312,143 - H281DRAFT_04192 0.55 -1.1
Or see this region's nucleotide sequence