Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04144 and H281DRAFT_04145 are separated by 112 nucleotides H281DRAFT_04145 and H281DRAFT_04146 overlap by 4 nucleotides H281DRAFT_04146 and H281DRAFT_04147 are separated by 46 nucleotides
H281DRAFT_04144: H281DRAFT_04144 - Tryptophan 2,3-dioxygenase holoenzyme /Tryptophan 2,3-dioxygenase apoenzyme, at 253,869 to 254,810
_04144
H281DRAFT_04145: H281DRAFT_04145 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 254,923 to 255,660
_04145
H281DRAFT_04146: H281DRAFT_04146 - xylulokinase, at 255,657 to 257,201
_04146
H281DRAFT_04147: H281DRAFT_04147 - L-iditol 2-dehydrogenase, at 257,248 to 258,345
_04147
Position (kb) 255
256
257
258 Strain fitness (log2 ratio) -2
-1
0
1 at 254.801 kb on + strand at 254.802 kb on - strand at 254.808 kb on + strand at 254.819 kb on + strand at 254.819 kb on + strand at 254.819 kb on + strand at 254.974 kb on - strand at 255.048 kb on + strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.103 kb on - strand, within H281DRAFT_04145 at 255.212 kb on + strand, within H281DRAFT_04145 at 255.651 kb on + strand at 255.652 kb on - strand at 255.652 kb on - strand at 255.712 kb on + strand at 255.734 kb on - strand at 255.881 kb on - strand, within H281DRAFT_04146 at 255.996 kb on + strand, within H281DRAFT_04146 at 256.307 kb on - strand, within H281DRAFT_04146 at 256.382 kb on - strand, within H281DRAFT_04146 at 256.796 kb on - strand, within H281DRAFT_04146 at 256.826 kb on - strand, within H281DRAFT_04146 at 256.894 kb on - strand, within H281DRAFT_04146 at 256.906 kb on + strand, within H281DRAFT_04146 at 256.919 kb on + strand, within H281DRAFT_04146 at 256.925 kb on + strand, within H281DRAFT_04146 at 257.104 kb on + strand at 257.104 kb on + strand at 257.194 kb on + strand at 257.215 kb on - strand at 257.216 kb on + strand at 257.270 kb on - strand at 257.568 kb on + strand, within H281DRAFT_04147 at 257.569 kb on - strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.570 kb on + strand, within H281DRAFT_04147 at 257.571 kb on - strand, within H281DRAFT_04147 at 257.572 kb on + strand, within H281DRAFT_04147 at 257.904 kb on + strand, within H281DRAFT_04147 at 257.905 kb on - strand, within H281DRAFT_04147 at 258.000 kb on - strand, within H281DRAFT_04147
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 254,801 + -0.5 254,802 - -0.2 254,808 + -0.2 254,819 + +0.3 254,819 + +0.8 254,819 + +1.0 254,974 - -0.6 255,048 + H281DRAFT_04145 0.17 +0.1 255,103 - H281DRAFT_04145 0.24 -0.4 255,103 - H281DRAFT_04145 0.24 -0.0 255,103 - H281DRAFT_04145 0.24 +1.5 255,103 - H281DRAFT_04145 0.24 -0.5 255,103 - H281DRAFT_04145 0.24 -1.8 255,212 + H281DRAFT_04145 0.39 +0.7 255,651 + -0.0 255,652 - -0.7 255,652 - -0.2 255,712 + -0.2 255,734 - -0.3 255,881 - H281DRAFT_04146 0.14 -1.8 255,996 + H281DRAFT_04146 0.22 -0.4 256,307 - H281DRAFT_04146 0.42 +0.4 256,382 - H281DRAFT_04146 0.47 -0.2 256,796 - H281DRAFT_04146 0.74 -1.2 256,826 - H281DRAFT_04146 0.76 -0.7 256,894 - H281DRAFT_04146 0.80 -1.3 256,906 + H281DRAFT_04146 0.81 -1.4 256,919 + H281DRAFT_04146 0.82 +0.2 256,925 + H281DRAFT_04146 0.82 -1.7 257,104 + +1.0 257,104 + +0.2 257,194 + -1.6 257,215 - -0.0 257,216 + -0.8 257,270 - +0.0 257,568 + H281DRAFT_04147 0.29 -0.1 257,569 - H281DRAFT_04147 0.29 -0.1 257,570 + H281DRAFT_04147 0.29 +1.3 257,570 + H281DRAFT_04147 0.29 -0.3 257,570 + H281DRAFT_04147 0.29 -0.4 257,571 - H281DRAFT_04147 0.29 -0.1 257,572 + H281DRAFT_04147 0.30 -0.5 257,904 + H281DRAFT_04147 0.60 -1.8 257,905 - H281DRAFT_04147 0.60 +0.6 258,000 - H281DRAFT_04147 0.68 +0.1
Or see this region's nucleotide sequence