Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04084 and H281DRAFT_04085 are separated by 295 nucleotides H281DRAFT_04085 and H281DRAFT_04086 are separated by 237 nucleotides
H281DRAFT_04084: H281DRAFT_04084 - cold-shock DNA-binding protein family, at 188,790 to 188,996
_04084
H281DRAFT_04085: H281DRAFT_04085 - isocitrate dehydrogenase (NADP), at 189,292 to 190,548
_04085
H281DRAFT_04086: H281DRAFT_04086 - 23S rRNA pseudouridine2457 synthase, at 190,786 to 191,448
_04086
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-1
0
1
2 at 188.383 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.556 kb on - strand at 188.603 kb on - strand at 188.636 kb on - strand at 188.774 kb on + strand at 188.775 kb on - strand at 189.089 kb on + strand at 189.090 kb on - strand at 189.188 kb on + strand at 189.293 kb on + strand at 189.293 kb on + strand at 189.294 kb on - strand at 189.371 kb on + strand at 189.444 kb on + strand, within H281DRAFT_04085 at 189.494 kb on + strand, within H281DRAFT_04085 at 189.494 kb on + strand, within H281DRAFT_04085 at 189.495 kb on - strand, within H281DRAFT_04085 at 189.509 kb on + strand, within H281DRAFT_04085 at 189.510 kb on - strand, within H281DRAFT_04085 at 189.561 kb on + strand, within H281DRAFT_04085 at 189.562 kb on - strand, within H281DRAFT_04085 at 189.620 kb on + strand, within H281DRAFT_04085 at 189.620 kb on + strand, within H281DRAFT_04085 at 189.621 kb on - strand, within H281DRAFT_04085 at 189.656 kb on + strand, within H281DRAFT_04085 at 189.656 kb on + strand, within H281DRAFT_04085 at 189.657 kb on - strand, within H281DRAFT_04085 at 189.813 kb on - strand, within H281DRAFT_04085 at 189.836 kb on + strand, within H281DRAFT_04085 at 189.951 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.064 kb on + strand, within H281DRAFT_04085 at 190.065 kb on - strand, within H281DRAFT_04085 at 190.145 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.169 kb on + strand, within H281DRAFT_04085 at 190.170 kb on - strand, within H281DRAFT_04085 at 190.170 kb on - strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.259 kb on + strand, within H281DRAFT_04085 at 190.260 kb on - strand, within H281DRAFT_04085 at 190.260 kb on - strand, within H281DRAFT_04085 at 190.337 kb on + strand, within H281DRAFT_04085 at 190.373 kb on + strand, within H281DRAFT_04085 at 190.374 kb on - strand, within H281DRAFT_04085 at 190.419 kb on + strand, within H281DRAFT_04085 at 190.541 kb on + strand at 190.542 kb on - strand at 190.653 kb on - strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.747 kb on + strand at 190.751 kb on + strand at 190.778 kb on + strand at 190.779 kb on - strand at 190.786 kb on - strand at 190.786 kb on - strand at 190.803 kb on + strand at 190.804 kb on - strand at 190.804 kb on - strand at 190.878 kb on + strand, within H281DRAFT_04086 at 190.878 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.073 kb on + strand, within H281DRAFT_04086 at 191.074 kb on - strand, within H281DRAFT_04086 at 191.164 kb on + strand, within H281DRAFT_04086 at 191.164 kb on + strand, within H281DRAFT_04086 at 191.165 kb on - strand, within H281DRAFT_04086 at 191.191 kb on + strand, within H281DRAFT_04086 at 191.209 kb on - strand, within H281DRAFT_04086 at 191.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 188,383 - -0.0 188,556 - +0.1 188,556 - +0.6 188,556 - +0.4 188,603 - -0.0 188,636 - -0.9 188,774 + -0.8 188,775 - -0.6 189,089 + -0.2 189,090 - -0.2 189,188 + -0.1 189,293 + +0.2 189,293 + +0.5 189,294 - +0.8 189,371 + +0.7 189,444 + H281DRAFT_04085 0.12 +0.3 189,494 + H281DRAFT_04085 0.16 +0.2 189,494 + H281DRAFT_04085 0.16 -0.0 189,495 - H281DRAFT_04085 0.16 -1.0 189,509 + H281DRAFT_04085 0.17 +0.5 189,510 - H281DRAFT_04085 0.17 +0.4 189,561 + H281DRAFT_04085 0.21 +0.7 189,562 - H281DRAFT_04085 0.21 -0.2 189,620 + H281DRAFT_04085 0.26 +0.4 189,620 + H281DRAFT_04085 0.26 +0.0 189,621 - H281DRAFT_04085 0.26 -0.6 189,656 + H281DRAFT_04085 0.29 +0.1 189,656 + H281DRAFT_04085 0.29 -1.1 189,657 - H281DRAFT_04085 0.29 +0.7 189,813 - H281DRAFT_04085 0.41 +0.2 189,836 + H281DRAFT_04085 0.43 +0.2 189,951 + H281DRAFT_04085 0.52 +1.2 190,064 + H281DRAFT_04085 0.61 -0.6 190,064 + H281DRAFT_04085 0.61 +0.1 190,064 + H281DRAFT_04085 0.61 +1.0 190,064 + H281DRAFT_04085 0.61 +0.2 190,065 - H281DRAFT_04085 0.61 -0.1 190,145 + H281DRAFT_04085 0.68 -0.4 190,169 + H281DRAFT_04085 0.70 +0.3 190,169 + H281DRAFT_04085 0.70 -0.5 190,169 + H281DRAFT_04085 0.70 -0.6 190,169 + H281DRAFT_04085 0.70 -0.3 190,169 + H281DRAFT_04085 0.70 +0.1 190,169 + H281DRAFT_04085 0.70 -0.0 190,169 + H281DRAFT_04085 0.70 +0.7 190,170 - H281DRAFT_04085 0.70 +0.7 190,170 - H281DRAFT_04085 0.70 -0.6 190,259 + H281DRAFT_04085 0.77 -0.8 190,259 + H281DRAFT_04085 0.77 -0.8 190,259 + H281DRAFT_04085 0.77 +0.3 190,259 + H281DRAFT_04085 0.77 +0.1 190,259 + H281DRAFT_04085 0.77 -0.2 190,260 - H281DRAFT_04085 0.77 -0.8 190,260 - H281DRAFT_04085 0.77 +0.7 190,337 + H281DRAFT_04085 0.83 -0.4 190,373 + H281DRAFT_04085 0.86 -0.0 190,374 - H281DRAFT_04085 0.86 +0.2 190,419 + H281DRAFT_04085 0.90 -0.3 190,541 + +0.5 190,542 - +0.1 190,653 - +0.1 190,747 + +1.6 190,747 + +0.6 190,747 + -0.2 190,747 + +0.2 190,751 + +0.4 190,778 + +0.2 190,779 - -0.0 190,786 - +1.6 190,786 - -0.6 190,803 + +0.3 190,804 - +0.5 190,804 - -0.0 190,878 + H281DRAFT_04086 0.14 +0.4 190,878 + H281DRAFT_04086 0.14 -0.5 191,073 + H281DRAFT_04086 0.43 +0.7 191,073 + H281DRAFT_04086 0.43 +1.1 191,073 + H281DRAFT_04086 0.43 -0.3 191,074 - H281DRAFT_04086 0.43 -0.3 191,164 + H281DRAFT_04086 0.57 +0.3 191,164 + H281DRAFT_04086 0.57 +0.1 191,165 - H281DRAFT_04086 0.57 +1.8 191,191 + H281DRAFT_04086 0.61 -0.4 191,209 - H281DRAFT_04086 0.64 +0.3 191,454 + -0.3
Or see this region's nucleotide sequence