Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04033 and H281DRAFT_04034 are separated by 106 nucleotides H281DRAFT_04034 and H281DRAFT_04035 are separated by 108 nucleotides H281DRAFT_04035 and H281DRAFT_04036 are separated by 415 nucleotides
H281DRAFT_04033: H281DRAFT_04033 - YggT family protein, at 134,882 to 135,445
_04033
H281DRAFT_04034: H281DRAFT_04034 - tRNA_Gly_CCC, at 135,552 to 135,625
_04034
H281DRAFT_04035: H281DRAFT_04035 - hypothetical protein, at 135,734 to 136,117
_04035
H281DRAFT_04036: H281DRAFT_04036 - tRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.33), at 136,533 to 137,321
_04036
Position (kb)
135
136
137 Strain fitness (log2 ratio)
-2
-1
0
1 at 134.800 kb on - strand at 134.800 kb on - strand at 134.870 kb on + strand at 134.870 kb on + strand at 134.870 kb on + strand at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.010 kb on - strand, within H281DRAFT_04033 at 135.431 kb on + strand at 135.431 kb on + strand at 135.431 kb on + strand at 135.432 kb on - strand at 135.490 kb on + strand at 135.542 kb on - strand at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.570 kb on + strand, within H281DRAFT_04034 at 135.571 kb on - strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.574 kb on - strand, within H281DRAFT_04034 at 135.579 kb on - strand, within H281DRAFT_04034 at 135.591 kb on + strand, within H281DRAFT_04034 at 135.628 kb on + strand at 135.628 kb on + strand at 135.678 kb on + strand at 135.678 kb on + strand at 135.679 kb on - strand at 135.731 kb on - strand at 135.787 kb on - strand, within H281DRAFT_04035 at 135.799 kb on + strand, within H281DRAFT_04035 at 135.801 kb on + strand, within H281DRAFT_04035 at 135.871 kb on - strand, within H281DRAFT_04035 at 136.034 kb on - strand, within H281DRAFT_04035 at 136.034 kb on - strand, within H281DRAFT_04035 at 136.055 kb on - strand, within H281DRAFT_04035 at 136.111 kb on + strand at 136.112 kb on - strand at 136.112 kb on - strand at 136.117 kb on + strand at 136.117 kb on + strand at 136.118 kb on - strand at 136.122 kb on + strand at 136.131 kb on - strand at 136.131 kb on - strand at 136.135 kb on + strand at 136.136 kb on - strand at 136.157 kb on + strand at 136.158 kb on - strand at 136.168 kb on + strand at 136.169 kb on - strand at 136.175 kb on + strand at 136.176 kb on - strand at 136.196 kb on - strand at 136.202 kb on + strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.203 kb on - strand at 136.268 kb on - strand at 136.268 kb on - strand at 136.322 kb on - strand at 136.343 kb on - strand at 136.371 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.519 kb on + strand at 136.520 kb on - strand at 136.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 134,800 - +0.1 134,800 - -0.4 134,870 + -0.6 134,870 + -0.8 134,870 + +0.3 135,009 + H281DRAFT_04033 0.23 -0.3 135,009 + H281DRAFT_04033 0.23 -0.8 135,009 + H281DRAFT_04033 0.23 -0.6 135,009 + H281DRAFT_04033 0.23 -0.2 135,010 - H281DRAFT_04033 0.23 +0.5 135,431 + -0.6 135,431 + -0.6 135,431 + +0.4 135,432 - -0.6 135,490 + +0.4 135,542 - -0.2 135,562 + H281DRAFT_04034 0.14 +0.5 135,562 + H281DRAFT_04034 0.14 +0.3 135,562 + H281DRAFT_04034 0.14 -0.1 135,565 - H281DRAFT_04034 0.18 -0.3 135,565 - H281DRAFT_04034 0.18 +0.2 135,570 + H281DRAFT_04034 0.24 -0.7 135,571 - H281DRAFT_04034 0.26 +0.3 135,573 + H281DRAFT_04034 0.28 +0.4 135,573 + H281DRAFT_04034 0.28 -0.2 135,573 + H281DRAFT_04034 0.28 +0.6 135,574 - H281DRAFT_04034 0.30 +0.7 135,579 - H281DRAFT_04034 0.36 +0.2 135,591 + H281DRAFT_04034 0.53 +0.3 135,628 + +0.3 135,628 + +1.0 135,678 + +1.7 135,678 + +0.6 135,679 - -0.2 135,731 - -2.2 135,787 - H281DRAFT_04035 0.14 -0.2 135,799 + H281DRAFT_04035 0.17 -1.4 135,801 + H281DRAFT_04035 0.17 +0.4 135,871 - H281DRAFT_04035 0.36 +0.2 136,034 - H281DRAFT_04035 0.78 +0.0 136,034 - H281DRAFT_04035 0.78 +0.9 136,055 - H281DRAFT_04035 0.84 +0.7 136,111 + +0.1 136,112 - +0.4 136,112 - +0.8 136,117 + +0.0 136,117 + +0.0 136,118 - -0.1 136,122 + +0.3 136,131 - +0.7 136,131 - -0.5 136,135 + +1.4 136,136 - +0.3 136,157 + -0.1 136,158 - -0.6 136,168 + +1.3 136,169 - -0.4 136,175 + +0.2 136,176 - -1.1 136,196 - +0.7 136,202 + -0.5 136,203 - -0.0 136,203 - -0.6 136,203 - +0.6 136,203 - -0.4 136,268 - -0.2 136,268 - +0.2 136,322 - -0.2 136,343 - -0.4 136,371 + +0.2 136,519 + +0.4 136,519 + -0.1 136,519 + +1.1 136,519 + +1.2 136,520 - +1.3 136,520 - -0.9
Or see this region's nucleotide sequence