Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04031 and H281DRAFT_04032 are separated by 77 nucleotides H281DRAFT_04032 and H281DRAFT_04033 are separated by 197 nucleotides H281DRAFT_04033 and H281DRAFT_04034 are separated by 106 nucleotides
H281DRAFT_04031: H281DRAFT_04031 - Predicted oxidoreductase, contains short-chain dehydrogenase (SDR) and DUF2520 domains, at 133,345 to 134,286
_04031
H281DRAFT_04032: H281DRAFT_04032 - conserved hypothetical protein, at 134,364 to 134,684
_04032
H281DRAFT_04033: H281DRAFT_04033 - YggT family protein, at 134,882 to 135,445
_04033
H281DRAFT_04034: H281DRAFT_04034 - tRNA_Gly_CCC, at 135,552 to 135,625
_04034
Position (kb)
134
135 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 133.594 kb on - strand, within H281DRAFT_04031 at 133.594 kb on - strand, within H281DRAFT_04031 at 133.633 kb on + strand, within H281DRAFT_04031 at 133.732 kb on + strand, within H281DRAFT_04031 at 133.885 kb on + strand, within H281DRAFT_04031 at 133.885 kb on + strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.903 kb on + strand, within H281DRAFT_04031 at 133.903 kb on + strand, within H281DRAFT_04031 at 133.904 kb on - strand, within H281DRAFT_04031 at 133.904 kb on - strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031 at 134.284 kb on + strand at 134.285 kb on - strand at 134.294 kb on + strand at 134.294 kb on + strand at 134.294 kb on + strand at 134.294 kb on + strand at 134.295 kb on - strand at 134.421 kb on + strand, within H281DRAFT_04032 at 134.422 kb on - strand, within H281DRAFT_04032 at 134.422 kb on - strand, within H281DRAFT_04032 at 134.424 kb on + strand, within H281DRAFT_04032 at 134.425 kb on - strand, within H281DRAFT_04032 at 134.425 kb on - strand, within H281DRAFT_04032 at 134.532 kb on + strand, within H281DRAFT_04032 at 134.532 kb on + strand, within H281DRAFT_04032 at 134.533 kb on - strand, within H281DRAFT_04032 at 134.533 kb on - strand, within H281DRAFT_04032 at 134.533 kb on - strand, within H281DRAFT_04032 at 134.533 kb on - strand at 134.578 kb on - strand, within H281DRAFT_04032 at 134.628 kb on + strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.629 kb on - strand, within H281DRAFT_04032 at 134.800 kb on - strand at 134.800 kb on - strand at 134.870 kb on + strand at 134.870 kb on + strand at 134.870 kb on + strand at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.009 kb on + strand, within H281DRAFT_04033 at 135.010 kb on - strand, within H281DRAFT_04033 at 135.431 kb on + strand at 135.431 kb on + strand at 135.431 kb on + strand at 135.432 kb on - strand at 135.490 kb on + strand at 135.542 kb on - strand at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.562 kb on + strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.565 kb on - strand, within H281DRAFT_04034 at 135.570 kb on + strand, within H281DRAFT_04034 at 135.571 kb on - strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.573 kb on + strand, within H281DRAFT_04034 at 135.574 kb on - strand, within H281DRAFT_04034 at 135.579 kb on - strand, within H281DRAFT_04034 at 135.591 kb on + strand, within H281DRAFT_04034 at 135.628 kb on + strand at 135.628 kb on + strand at 135.678 kb on + strand at 135.678 kb on + strand at 135.679 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 133,594 - H281DRAFT_04031 0.26 -0.0 133,594 - H281DRAFT_04031 0.26 -0.5 133,633 + H281DRAFT_04031 0.31 +1.6 133,732 + H281DRAFT_04031 0.41 -0.8 133,885 + H281DRAFT_04031 0.57 +0.5 133,885 + H281DRAFT_04031 0.57 +1.0 133,886 - H281DRAFT_04031 0.57 -0.7 133,886 - H281DRAFT_04031 0.57 -0.2 133,886 - H281DRAFT_04031 0.57 +1.2 133,903 + H281DRAFT_04031 0.59 -1.6 133,903 + H281DRAFT_04031 0.59 +0.5 133,904 - H281DRAFT_04031 0.59 +0.4 133,904 - H281DRAFT_04031 0.59 +0.1 134,143 + H281DRAFT_04031 0.85 +0.5 134,143 + H281DRAFT_04031 0.85 +0.1 134,143 + H281DRAFT_04031 0.85 +0.8 134,284 + +0.1 134,285 - +0.3 134,294 + -1.3 134,294 + +0.8 134,294 + -0.4 134,294 + +0.1 134,295 - -0.5 134,421 + H281DRAFT_04032 0.18 -1.0 134,422 - H281DRAFT_04032 0.18 +0.1 134,422 - H281DRAFT_04032 0.18 -0.3 134,424 + H281DRAFT_04032 0.19 -0.2 134,425 - H281DRAFT_04032 0.19 -0.3 134,425 - H281DRAFT_04032 0.19 -1.9 134,532 + H281DRAFT_04032 0.52 +1.0 134,532 + H281DRAFT_04032 0.52 +0.6 134,533 - H281DRAFT_04032 0.53 -0.5 134,533 - H281DRAFT_04032 0.53 -0.6 134,533 - H281DRAFT_04032 0.53 -3.3 134,533 - -1.6 134,578 - H281DRAFT_04032 0.67 -0.3 134,628 + H281DRAFT_04032 0.82 +0.2 134,629 - H281DRAFT_04032 0.83 -0.6 134,629 - H281DRAFT_04032 0.83 -0.0 134,629 - H281DRAFT_04032 0.83 +0.5 134,629 - H281DRAFT_04032 0.83 +0.6 134,800 - +0.1 134,800 - -0.4 134,870 + -0.6 134,870 + -0.8 134,870 + +0.3 135,009 + H281DRAFT_04033 0.23 -0.3 135,009 + H281DRAFT_04033 0.23 -0.8 135,009 + H281DRAFT_04033 0.23 -0.6 135,009 + H281DRAFT_04033 0.23 -0.2 135,010 - H281DRAFT_04033 0.23 +0.5 135,431 + -0.6 135,431 + -0.6 135,431 + +0.4 135,432 - -0.6 135,490 + +0.4 135,542 - -0.2 135,562 + H281DRAFT_04034 0.14 +0.5 135,562 + H281DRAFT_04034 0.14 +0.3 135,562 + H281DRAFT_04034 0.14 -0.1 135,565 - H281DRAFT_04034 0.18 -0.3 135,565 - H281DRAFT_04034 0.18 +0.2 135,570 + H281DRAFT_04034 0.24 -0.7 135,571 - H281DRAFT_04034 0.26 +0.3 135,573 + H281DRAFT_04034 0.28 +0.4 135,573 + H281DRAFT_04034 0.28 -0.2 135,573 + H281DRAFT_04034 0.28 +0.6 135,574 - H281DRAFT_04034 0.30 +0.7 135,579 - H281DRAFT_04034 0.36 +0.2 135,591 + H281DRAFT_04034 0.53 +0.3 135,628 + +0.3 135,628 + +1.0 135,678 + +1.7 135,678 + +0.6 135,679 - -0.2
Or see this region's nucleotide sequence