Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04028 and H281DRAFT_04029 are separated by 208 nucleotides H281DRAFT_04029 and H281DRAFT_04030 are separated by 368 nucleotides H281DRAFT_04030 and H281DRAFT_04031 are separated by 119 nucleotides
H281DRAFT_04028: H281DRAFT_04028 - threonine dehydratase, at 131,078 to 132,052
_04028
H281DRAFT_04029: H281DRAFT_04029 - hypothetical protein, at 132,261 to 132,575
_04029
H281DRAFT_04030: H281DRAFT_04030 - hypothetical protein, at 132,944 to 133,225
_04030
H281DRAFT_04031: H281DRAFT_04031 - Predicted oxidoreductase, contains short-chain dehydrogenase (SDR) and DUF2520 domains, at 133,345 to 134,286
_04031
Position (kb) 132
133
134 Strain fitness (log2 ratio) -2
-1
0
1
2 at 132.141 kb on + strand at 132.180 kb on + strand at 132.181 kb on - strand at 132.188 kb on + strand at 132.206 kb on + strand at 132.263 kb on - strand at 132.272 kb on - strand at 132.272 kb on - strand at 132.298 kb on + strand, within H281DRAFT_04029 at 132.298 kb on + strand, within H281DRAFT_04029 at 132.301 kb on + strand, within H281DRAFT_04029 at 132.314 kb on - strand, within H281DRAFT_04029 at 132.317 kb on - strand, within H281DRAFT_04029 at 132.334 kb on + strand, within H281DRAFT_04029 at 132.334 kb on + strand, within H281DRAFT_04029 at 132.334 kb on + strand, within H281DRAFT_04029 at 132.334 kb on + strand, within H281DRAFT_04029 at 132.335 kb on - strand, within H281DRAFT_04029 at 132.335 kb on - strand at 132.337 kb on + strand, within H281DRAFT_04029 at 132.338 kb on - strand, within H281DRAFT_04029 at 132.338 kb on - strand, within H281DRAFT_04029 at 132.367 kb on + strand, within H281DRAFT_04029 at 132.388 kb on + strand, within H281DRAFT_04029 at 132.413 kb on - strand at 132.416 kb on - strand, within H281DRAFT_04029 at 132.437 kb on - strand, within H281DRAFT_04029 at 132.451 kb on + strand, within H281DRAFT_04029 at 132.452 kb on - strand, within H281DRAFT_04029 at 132.461 kb on - strand, within H281DRAFT_04029 at 132.461 kb on - strand, within H281DRAFT_04029 at 132.537 kb on + strand, within H281DRAFT_04029 at 132.631 kb on + strand at 132.632 kb on - strand at 132.632 kb on - strand at 132.692 kb on + strand at 132.771 kb on - strand at 132.844 kb on + strand at 132.848 kb on + strand at 132.855 kb on + strand at 132.856 kb on - strand at 132.892 kb on - strand at 132.892 kb on - strand at 132.922 kb on + strand at 132.960 kb on + strand at 132.961 kb on - strand at 132.993 kb on + strand, within H281DRAFT_04030 at 132.993 kb on + strand, within H281DRAFT_04030 at 132.994 kb on - strand, within H281DRAFT_04030 at 133.100 kb on - strand at 133.109 kb on - strand, within H281DRAFT_04030 at 133.109 kb on - strand, within H281DRAFT_04030 at 133.147 kb on - strand, within H281DRAFT_04030 at 133.152 kb on + strand, within H281DRAFT_04030 at 133.152 kb on + strand, within H281DRAFT_04030 at 133.152 kb on + strand, within H281DRAFT_04030 at 133.153 kb on - strand, within H281DRAFT_04030 at 133.153 kb on - strand, within H281DRAFT_04030 at 133.304 kb on - strand at 133.594 kb on - strand, within H281DRAFT_04031 at 133.594 kb on - strand, within H281DRAFT_04031 at 133.633 kb on + strand, within H281DRAFT_04031 at 133.732 kb on + strand, within H281DRAFT_04031 at 133.885 kb on + strand, within H281DRAFT_04031 at 133.885 kb on + strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.886 kb on - strand, within H281DRAFT_04031 at 133.903 kb on + strand, within H281DRAFT_04031 at 133.903 kb on + strand, within H281DRAFT_04031 at 133.904 kb on - strand, within H281DRAFT_04031 at 133.904 kb on - strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031 at 134.143 kb on + strand, within H281DRAFT_04031
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 132,141 + -0.6 132,180 + -0.1 132,181 - -1.5 132,188 + +0.2 132,206 + -0.6 132,263 - -1.1 132,272 - +0.4 132,272 - -0.5 132,298 + H281DRAFT_04029 0.12 +0.2 132,298 + H281DRAFT_04029 0.12 +0.2 132,301 + H281DRAFT_04029 0.13 +0.1 132,314 - H281DRAFT_04029 0.17 +0.1 132,317 - H281DRAFT_04029 0.18 +0.2 132,334 + H281DRAFT_04029 0.23 +0.6 132,334 + H281DRAFT_04029 0.23 +0.4 132,334 + H281DRAFT_04029 0.23 +0.8 132,334 + H281DRAFT_04029 0.23 +0.0 132,335 - H281DRAFT_04029 0.23 -0.3 132,335 - -0.0 132,337 + H281DRAFT_04029 0.24 +1.6 132,338 - H281DRAFT_04029 0.24 -0.7 132,338 - H281DRAFT_04029 0.24 -0.5 132,367 + H281DRAFT_04029 0.34 +0.1 132,388 + H281DRAFT_04029 0.40 +0.4 132,413 - +1.0 132,416 - H281DRAFT_04029 0.49 +0.4 132,437 - H281DRAFT_04029 0.56 +0.4 132,451 + H281DRAFT_04029 0.60 +2.0 132,452 - H281DRAFT_04029 0.61 +0.3 132,461 - H281DRAFT_04029 0.63 -0.4 132,461 - H281DRAFT_04029 0.63 -0.6 132,537 + H281DRAFT_04029 0.88 -0.5 132,631 + +1.8 132,632 - +0.4 132,632 - +0.6 132,692 + +0.6 132,771 - -0.3 132,844 + +0.6 132,848 + +0.4 132,855 + +0.4 132,856 - +0.8 132,892 - -1.5 132,892 - -2.0 132,922 + +0.4 132,960 + +0.1 132,961 - -2.5 132,993 + H281DRAFT_04030 0.17 +0.5 132,993 + H281DRAFT_04030 0.17 +0.2 132,994 - H281DRAFT_04030 0.18 +0.1 133,100 - +2.0 133,109 - H281DRAFT_04030 0.59 +1.4 133,109 - H281DRAFT_04030 0.59 -0.4 133,147 - H281DRAFT_04030 0.72 +0.4 133,152 + H281DRAFT_04030 0.74 +0.3 133,152 + H281DRAFT_04030 0.74 +0.2 133,152 + H281DRAFT_04030 0.74 +0.9 133,153 - H281DRAFT_04030 0.74 +0.4 133,153 - H281DRAFT_04030 0.74 -0.1 133,304 - -0.5 133,594 - H281DRAFT_04031 0.26 -0.0 133,594 - H281DRAFT_04031 0.26 -0.5 133,633 + H281DRAFT_04031 0.31 +1.6 133,732 + H281DRAFT_04031 0.41 -0.8 133,885 + H281DRAFT_04031 0.57 +0.5 133,885 + H281DRAFT_04031 0.57 +1.0 133,886 - H281DRAFT_04031 0.57 -0.7 133,886 - H281DRAFT_04031 0.57 -0.2 133,886 - H281DRAFT_04031 0.57 +1.2 133,903 + H281DRAFT_04031 0.59 -1.6 133,903 + H281DRAFT_04031 0.59 +0.5 133,904 - H281DRAFT_04031 0.59 +0.4 133,904 - H281DRAFT_04031 0.59 +0.1 134,143 + H281DRAFT_04031 0.85 +0.5 134,143 + H281DRAFT_04031 0.85 +0.1 134,143 + H281DRAFT_04031 0.85 +0.8
Or see this region's nucleotide sequence