Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04019 and H281DRAFT_04020 are separated by 11 nucleotides H281DRAFT_04020 and H281DRAFT_04021 are separated by 254 nucleotides H281DRAFT_04021 and H281DRAFT_04022 are separated by 56 nucleotides H281DRAFT_04022 and H281DRAFT_04023 overlap by 4 nucleotides
H281DRAFT_04019: H281DRAFT_04019 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 121,150 to 122,307
_04019
H281DRAFT_04020: H281DRAFT_04020 - riboflavin synthase alpha chain, at 122,319 to 122,933
_04020
H281DRAFT_04021: H281DRAFT_04021 - 3,4-dihydroxy-2-butanone 4-phosphate synthase /GTP cyclohydrolase II, at 123,188 to 124,318
_04021
H281DRAFT_04022: H281DRAFT_04022 - 6,7-dimethyl-8-ribityllumazine synthase, at 124,375 to 124,878
_04022
H281DRAFT_04023: H281DRAFT_04023 - NusB antitermination factor, at 124,875 to 125,312
_04023
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1 at 122.312 kb on + strand at 123.075 kb on - strand at 123.075 kb on - strand at 123.075 kb on - strand at 123.115 kb on + strand at 123.115 kb on + strand at 123.116 kb on - strand at 123.155 kb on + strand at 123.205 kb on + strand at 123.205 kb on + strand at 123.205 kb on + strand at 123.428 kb on + strand, within H281DRAFT_04021 at 123.547 kb on + strand, within H281DRAFT_04021 at 123.811 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 123.863 kb on + strand, within H281DRAFT_04021 at 125.310 kb on + strand at 125.311 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 122,312 + -0.2 123,075 - +0.4 123,075 - +0.2 123,075 - -1.0 123,115 + -0.6 123,115 + +0.2 123,116 - +1.0 123,155 + -0.1 123,205 + -0.6 123,205 + -0.5 123,205 + -0.5 123,428 + H281DRAFT_04021 0.21 -0.3 123,547 + H281DRAFT_04021 0.32 -1.0 123,811 + H281DRAFT_04021 0.55 -1.8 123,863 + H281DRAFT_04021 0.60 -2.7 123,863 + H281DRAFT_04021 0.60 -1.2 123,863 + H281DRAFT_04021 0.60 -0.4 125,310 + -0.3 125,311 - +0.6
Or see this region's nucleotide sequence