Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03725 and H281DRAFT_03726 are separated by 129 nucleotides H281DRAFT_03726 and H281DRAFT_03727 are separated by 8 nucleotides H281DRAFT_03727 and H281DRAFT_03728 are separated by 33 nucleotides H281DRAFT_03728 and H281DRAFT_03729 are separated by 98 nucleotides
H281DRAFT_03725: H281DRAFT_03725 - hypothetical protein, at 239,662 to 240,882
_03725
H281DRAFT_03726: H281DRAFT_03726 - Predicted dithiol-disulfide oxidoreductase, DUF899 family, at 241,012 to 241,779
_03726
H281DRAFT_03727: H281DRAFT_03727 - Predicted metal-binding membrane protein, at 241,788 to 242,633
_03727
H281DRAFT_03728: H281DRAFT_03728 - Uncharacterized conserved protein, DUF1697 family, at 242,667 to 243,176
_03728
H281DRAFT_03729: H281DRAFT_03729 - carbohydrate ABC transporter substrate-binding protein, CUT1 family, at 243,275 to 245,014
_03729
Position (kb)
241
242
243 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 240.936 kb on + strand at 240.936 kb on + strand at 240.960 kb on + strand at 241.382 kb on - strand, within H281DRAFT_03726 at 241.462 kb on - strand, within H281DRAFT_03726 at 241.655 kb on - strand, within H281DRAFT_03726 at 241.655 kb on - strand, within H281DRAFT_03726 at 241.663 kb on + strand, within H281DRAFT_03726 at 241.664 kb on - strand, within H281DRAFT_03726 at 241.697 kb on - strand, within H281DRAFT_03726 at 242.147 kb on + strand, within H281DRAFT_03727 at 242.412 kb on + strand, within H281DRAFT_03727 at 242.413 kb on - strand, within H281DRAFT_03727 at 242.425 kb on + strand, within H281DRAFT_03727 at 242.526 kb on - strand, within H281DRAFT_03727 at 243.070 kb on - strand, within H281DRAFT_03728 at 243.318 kb on + strand at 243.567 kb on + strand, within H281DRAFT_03729 at 243.621 kb on + strand, within H281DRAFT_03729 at 243.622 kb on - strand, within H281DRAFT_03729
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 240,936 + +2.7 240,936 + -1.3 240,960 + +0.3 241,382 - H281DRAFT_03726 0.48 +0.2 241,462 - H281DRAFT_03726 0.59 -0.0 241,655 - H281DRAFT_03726 0.84 +0.3 241,655 - H281DRAFT_03726 0.84 -1.9 241,663 + H281DRAFT_03726 0.85 -0.4 241,664 - H281DRAFT_03726 0.85 +0.5 241,697 - H281DRAFT_03726 0.89 -0.0 242,147 + H281DRAFT_03727 0.42 +0.0 242,412 + H281DRAFT_03727 0.74 -0.0 242,413 - H281DRAFT_03727 0.74 +0.6 242,425 + H281DRAFT_03727 0.75 +0.8 242,526 - H281DRAFT_03727 0.87 +0.3 243,070 - H281DRAFT_03728 0.79 +0.6 243,318 + +0.4 243,567 + H281DRAFT_03729 0.17 +0.7 243,621 + H281DRAFT_03729 0.20 -0.2 243,622 - H281DRAFT_03729 0.20 -0.4
Or see this region's nucleotide sequence