Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_03256

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_03254 and H281DRAFT_03255 are separated by 20 nucleotidesH281DRAFT_03255 and H281DRAFT_03256 are separated by 62 nucleotidesH281DRAFT_03256 and H281DRAFT_03257 are separated by 28 nucleotidesH281DRAFT_03257 and H281DRAFT_03258 overlap by 4 nucleotides H281DRAFT_03254: H281DRAFT_03254 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 310,687 to 311,088 _03254 H281DRAFT_03255: H281DRAFT_03255 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 311,109 to 311,972 _03255 H281DRAFT_03256: H281DRAFT_03256 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 312,035 to 313,492 _03256 H281DRAFT_03257: H281DRAFT_03257 - 5-carboxy-2-oxohept-3-enedioate decarboxylase HpaG2 subunit, at 313,521 to 314,279 _03257 H281DRAFT_03258: H281DRAFT_03258 - 5-carboxy-2-oxohept-3-enedioate decarboxylase HpaG1 subunit (EC 4.1.1.68), at 314,276 to 315,049 _03258 Position (kb) 312 313 314Strain fitness (log2 ratio) -1 0 1at 311.077 kb on + strandat 311.174 kb on - strandat 311.203 kb on + strand, within H281DRAFT_03255at 311.574 kb on - strand, within H281DRAFT_03255at 311.575 kb on + strand, within H281DRAFT_03255at 311.704 kb on + strand, within H281DRAFT_03255at 312.376 kb on + strand, within H281DRAFT_03256at 312.505 kb on - strand, within H281DRAFT_03256at 313.684 kb on + strand, within H281DRAFT_03257

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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311,077 + +0.8
311,174 - +0.8
311,203 + H281DRAFT_03255 0.11 -0.9
311,574 - H281DRAFT_03255 0.54 -0.9
311,575 + H281DRAFT_03255 0.54 +0.8
311,704 + H281DRAFT_03255 0.69 -0.2
312,376 + H281DRAFT_03256 0.23 +0.4
312,505 - H281DRAFT_03256 0.32 +0.8
313,684 + H281DRAFT_03257 0.21 -1.6

Or see this region's nucleotide sequence