Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03139 and H281DRAFT_03140 are separated by 210 nucleotides H281DRAFT_03140 and H281DRAFT_03141 are separated by 82 nucleotides H281DRAFT_03141 and H281DRAFT_03142 are separated by 266 nucleotides
H281DRAFT_03139: H281DRAFT_03139 - membrane protein, at 176,316 to 177,641
_03139
H281DRAFT_03140: H281DRAFT_03140 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 177,852 to 178,391
_03140
H281DRAFT_03141: H281DRAFT_03141 - sulfate permease, SulP family, at 178,474 to 179,961
_03141
H281DRAFT_03142: H281DRAFT_03142 - Lipid-binding SYLF domain-containing protein, at 180,228 to 180,818
_03142
Position (kb)
178
179
180 Strain fitness (log2 ratio)
-1
0
1
2 at 177.661 kb on - strand at 177.747 kb on + strand at 177.747 kb on + strand at 177.748 kb on - strand at 177.748 kb on - strand at 177.749 kb on + strand at 177.750 kb on - strand at 177.750 kb on - strand at 177.811 kb on + strand at 177.827 kb on - strand at 177.835 kb on + strand at 177.835 kb on + strand at 177.836 kb on - strand at 177.836 kb on - strand at 177.836 kb on - strand at 177.842 kb on - strand at 177.842 kb on - strand at 178.040 kb on + strand, within H281DRAFT_03140 at 178.041 kb on - strand, within H281DRAFT_03140 at 178.041 kb on - strand, within H281DRAFT_03140 at 178.057 kb on - strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.081 kb on - strand, within H281DRAFT_03140 at 178.218 kb on - strand, within H281DRAFT_03140 at 178.516 kb on - strand at 178.516 kb on - strand at 178.718 kb on + strand, within H281DRAFT_03141 at 178.718 kb on + strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.725 kb on + strand, within H281DRAFT_03141 at 178.725 kb on + strand, within H281DRAFT_03141 at 179.003 kb on + strand, within H281DRAFT_03141 at 179.003 kb on + strand, within H281DRAFT_03141 at 179.004 kb on - strand, within H281DRAFT_03141 at 179.249 kb on + strand, within H281DRAFT_03141 at 179.249 kb on + strand, within H281DRAFT_03141 at 179.352 kb on + strand, within H281DRAFT_03141 at 179.442 kb on - strand, within H281DRAFT_03141 at 179.668 kb on + strand, within H281DRAFT_03141 at 179.688 kb on - strand, within H281DRAFT_03141 at 179.944 kb on - strand at 179.956 kb on - strand at 180.404 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.506 kb on - strand at 180.506 kb on - strand, within H281DRAFT_03142 at 180.649 kb on + strand at 180.821 kb on + strand at 180.822 kb on - strand at 180.822 kb on - strand at 180.833 kb on + strand at 180.833 kb on + strand at 180.833 kb on + strand at 180.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 177,661 - -0.7 177,747 + +0.1 177,747 + -0.5 177,748 - +0.5 177,748 - +0.3 177,749 + +0.0 177,750 - -0.2 177,750 - -0.4 177,811 + +0.1 177,827 - -0.6 177,835 + -0.1 177,835 + +0.4 177,836 - +1.0 177,836 - +0.3 177,836 - +1.1 177,842 - +0.2 177,842 - +0.3 178,040 + H281DRAFT_03140 0.35 -0.8 178,041 - H281DRAFT_03140 0.35 +0.7 178,041 - H281DRAFT_03140 0.35 +0.7 178,057 - H281DRAFT_03140 0.38 -0.8 178,080 + H281DRAFT_03140 0.42 +0.9 178,080 + H281DRAFT_03140 0.42 +0.4 178,080 + H281DRAFT_03140 0.42 +0.7 178,081 - H281DRAFT_03140 0.42 +0.2 178,218 - H281DRAFT_03140 0.68 +0.7 178,516 - -0.5 178,516 - +1.6 178,718 + H281DRAFT_03141 0.16 +2.1 178,718 + H281DRAFT_03141 0.16 +0.2 178,719 - H281DRAFT_03141 0.16 -1.3 178,719 - H281DRAFT_03141 0.16 -0.7 178,719 - H281DRAFT_03141 0.16 -0.8 178,719 - H281DRAFT_03141 0.16 -0.2 178,719 - H281DRAFT_03141 0.16 +2.0 178,725 + H281DRAFT_03141 0.17 -0.1 178,725 + H281DRAFT_03141 0.17 -1.4 179,003 + H281DRAFT_03141 0.36 +0.3 179,003 + H281DRAFT_03141 0.36 -0.6 179,004 - H281DRAFT_03141 0.36 +0.0 179,249 + H281DRAFT_03141 0.52 +0.3 179,249 + H281DRAFT_03141 0.52 -0.4 179,352 + H281DRAFT_03141 0.59 -0.7 179,442 - H281DRAFT_03141 0.65 +0.5 179,668 + H281DRAFT_03141 0.80 +0.1 179,688 - H281DRAFT_03141 0.82 +0.4 179,944 - +0.6 179,956 - -0.3 180,404 + H281DRAFT_03142 0.30 +0.6 180,505 + H281DRAFT_03142 0.47 +1.9 180,505 + H281DRAFT_03142 0.47 +0.2 180,505 + H281DRAFT_03142 0.47 +0.6 180,506 - +1.5 180,506 - H281DRAFT_03142 0.47 -0.6 180,649 + +0.9 180,821 + -0.3 180,822 - -0.5 180,822 - -0.4 180,833 + +0.1 180,833 + -0.3 180,833 + -0.3 180,833 + -0.0
Or see this region's nucleotide sequence