Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02409 and H281DRAFT_02410 are separated by 160 nucleotides H281DRAFT_02410 and H281DRAFT_02411 are separated by 78 nucleotides H281DRAFT_02411 and H281DRAFT_02414 are separated by 456 nucleotides H281DRAFT_02414 and H281DRAFT_02415 are separated by 182 nucleotides
H281DRAFT_02409: H281DRAFT_02409 - glycine dehydrogenase, at 580,799 to 583,735
_02409
H281DRAFT_02410: H281DRAFT_02410 - glycine cleavage system H protein, at 583,896 to 584,276
_02410
H281DRAFT_02411: H281DRAFT_02411 - aminomethyltransferase, at 584,355 to 585,473
_02411
H281DRAFT_02414: H281DRAFT_02414 - hypothetical protein, at 585,930 to 586,280
_02414
H281DRAFT_02415: H281DRAFT_02415 - Predicted dehydrogenase, at 586,463 to 587,512
_02415
Position (kb) 584
585
586 Strain fitness (log2 ratio) -2
-1
0
1
2 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.366 kb on - strand, within H281DRAFT_02409 at 583.391 kb on - strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.507 kb on + strand at 583.508 kb on - strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.885 kb on - strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.919 kb on - strand at 583.990 kb on + strand, within H281DRAFT_02410 at 583.991 kb on - strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand, within H281DRAFT_02410 at 584.146 kb on - strand, within H281DRAFT_02410 at 584.252 kb on - strand at 584.287 kb on + strand at 584.311 kb on + strand at 584.312 kb on - strand at 584.345 kb on + strand at 584.346 kb on - strand at 584.370 kb on + strand at 584.401 kb on - strand at 584.735 kb on + strand, within H281DRAFT_02411 at 584.746 kb on + strand, within H281DRAFT_02411 at 584.887 kb on + strand, within H281DRAFT_02411 at 584.888 kb on - strand, within H281DRAFT_02411 at 584.946 kb on - strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.160 kb on + strand, within H281DRAFT_02411 at 585.161 kb on - strand, within H281DRAFT_02411 at 585.164 kb on - strand, within H281DRAFT_02411 at 585.164 kb on - strand, within H281DRAFT_02411 at 585.379 kb on + strand at 585.379 kb on + strand at 585.574 kb on + strand at 585.731 kb on + strand at 585.731 kb on + strand at 585.731 kb on + strand at 585.732 kb on - strand at 585.732 kb on - strand at 585.891 kb on + strand at 586.088 kb on - strand, within H281DRAFT_02414 at 586.090 kb on + strand, within H281DRAFT_02414 at 586.091 kb on - strand, within H281DRAFT_02414 at 586.215 kb on + strand, within H281DRAFT_02414 at 586.243 kb on - strand, within H281DRAFT_02414 at 586.316 kb on + strand at 586.316 kb on + strand at 586.324 kb on + strand at 586.324 kb on + strand at 586.325 kb on - strand at 586.325 kb on - strand at 586.375 kb on + strand at 586.375 kb on + strand at 586.381 kb on + strand at 586.381 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.383 kb on + strand at 586.384 kb on - strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.447 kb on + strand at 586.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 583,357 - H281DRAFT_02409 0.87 +0.6 583,357 - H281DRAFT_02409 0.87 -0.7 583,357 - H281DRAFT_02409 0.87 -0.8 583,366 - H281DRAFT_02409 0.87 +0.1 583,391 - H281DRAFT_02409 0.88 -1.3 583,422 + H281DRAFT_02409 0.89 -0.5 583,422 + H281DRAFT_02409 0.89 -1.7 583,423 - H281DRAFT_02409 0.89 +0.9 583,423 - H281DRAFT_02409 0.89 -0.1 583,423 - H281DRAFT_02409 0.89 -0.4 583,441 - H281DRAFT_02409 0.90 -0.8 583,441 - H281DRAFT_02409 0.90 +0.6 583,507 + -1.3 583,508 - -1.2 583,884 + +0.4 583,884 + -0.8 583,884 + -1.1 583,884 + -0.2 583,884 + -0.5 583,885 - -0.3 583,886 + -1.7 583,886 + +1.5 583,886 + -0.7 583,886 + -0.4 583,887 - +0.2 583,887 - +0.1 583,887 - +0.1 583,919 - -0.7 583,990 + H281DRAFT_02410 0.25 -0.2 583,991 - H281DRAFT_02410 0.25 -0.3 584,068 + H281DRAFT_02410 0.45 -0.2 584,068 + H281DRAFT_02410 0.45 -1.5 584,069 - H281DRAFT_02410 0.45 -1.2 584,069 - H281DRAFT_02410 0.45 -0.4 584,069 - +0.1 584,145 + H281DRAFT_02410 0.65 -1.7 584,145 + -0.3 584,145 + H281DRAFT_02410 0.65 -0.5 584,145 + H281DRAFT_02410 0.65 -1.4 584,146 - H281DRAFT_02410 0.66 +0.1 584,252 - -0.9 584,287 + -1.9 584,311 + +0.6 584,312 - +0.3 584,345 + -1.1 584,346 - +0.4 584,370 + -1.0 584,401 - -0.4 584,735 + H281DRAFT_02411 0.34 -0.9 584,746 + H281DRAFT_02411 0.35 -0.9 584,887 + H281DRAFT_02411 0.48 +0.2 584,888 - H281DRAFT_02411 0.48 -1.0 584,946 - H281DRAFT_02411 0.53 -1.4 585,160 + H281DRAFT_02411 0.72 +0.0 585,160 + H281DRAFT_02411 0.72 -0.9 585,160 + H281DRAFT_02411 0.72 -1.0 585,161 - H281DRAFT_02411 0.72 -0.1 585,164 - H281DRAFT_02411 0.72 -2.5 585,164 - H281DRAFT_02411 0.72 +0.9 585,379 + -1.3 585,379 + -0.7 585,574 + +1.5 585,731 + -1.3 585,731 + -1.9 585,731 + -0.4 585,732 - -1.4 585,732 - +0.2 585,891 + -0.4 586,088 - H281DRAFT_02414 0.45 -0.1 586,090 + H281DRAFT_02414 0.46 -0.1 586,091 - H281DRAFT_02414 0.46 +0.2 586,215 + H281DRAFT_02414 0.81 +0.4 586,243 - H281DRAFT_02414 0.89 +0.2 586,316 + +2.4 586,316 + -0.0 586,324 + -0.4 586,324 + -0.3 586,325 - +1.2 586,325 - +0.1 586,375 + -0.6 586,375 + +0.6 586,381 + +0.3 586,381 + -0.3 586,383 + -0.4 586,383 + +1.1 586,383 + -0.4 586,383 + +0.0 586,383 + -0.7 586,384 - +0.2 586,447 + -0.5 586,447 + -0.5 586,447 + +2.5 586,447 + +0.2 586,448 - +0.5
Or see this region's nucleotide sequence