Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02230 and H281DRAFT_02231 are separated by 6 nucleotides H281DRAFT_02231 and H281DRAFT_02232 are separated by 72 nucleotides H281DRAFT_02232 and H281DRAFT_02233 are separated by 380 nucleotides H281DRAFT_02233 and H281DRAFT_02234 are separated by 95 nucleotides
H281DRAFT_02230: H281DRAFT_02230 - 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase, at 375,467 to 375,913
_02230
H281DRAFT_02231: H281DRAFT_02231 - 7-carboxy-7-deazaguanine synthase, Cx14CxxC type, at 375,920 to 376,552
_02231
H281DRAFT_02232: H281DRAFT_02232 - preQ(0) biosynthesis protein QueC, at 376,625 to 377,356
_02232
H281DRAFT_02233: H281DRAFT_02233 - tRNA_Phe_GAA, at 377,737 to 377,812
_02233
H281DRAFT_02234: H281DRAFT_02234 - two component transcriptional regulator, Fis family, at 377,908 to 378,600
_02234
Position (kb) 376
377
378 Strain fitness (log2 ratio) -1
0
1
2 at 375.822 kb on + strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.842 kb on - strand, within H281DRAFT_02230 at 375.936 kb on + strand at 375.936 kb on + strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 376.048 kb on - strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.522 kb on + strand at 376.522 kb on + strand at 376.522 kb on + strand at 376.523 kb on - strand at 376.523 kb on - strand at 376.524 kb on + strand at 376.525 kb on - strand at 376.574 kb on + strand at 376.749 kb on + strand, within H281DRAFT_02232 at 376.750 kb on - strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.766 kb on + strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.799 kb on + strand, within H281DRAFT_02232 at 376.800 kb on - strand, within H281DRAFT_02232 at 376.822 kb on + strand, within H281DRAFT_02232 at 377.045 kb on - strand, within H281DRAFT_02232 at 377.173 kb on + strand, within H281DRAFT_02232 at 377.173 kb on + strand at 377.180 kb on + strand, within H281DRAFT_02232 at 377.235 kb on + strand, within H281DRAFT_02232 at 377.235 kb on + strand, within H281DRAFT_02232 at 377.236 kb on - strand, within H281DRAFT_02232 at 377.266 kb on - strand, within H281DRAFT_02232 at 377.460 kb on + strand at 377.460 kb on + strand at 377.461 kb on - strand at 377.461 kb on - strand at 377.461 kb on - strand at 377.462 kb on + strand at 377.463 kb on - strand at 377.499 kb on + strand at 377.499 kb on + strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.500 kb on - strand at 377.501 kb on + strand at 377.502 kb on - strand at 377.614 kb on + strand at 377.653 kb on + strand at 377.653 kb on + strand at 377.654 kb on - strand at 377.656 kb on + strand at 377.657 kb on - strand at 377.657 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 375,822 + H281DRAFT_02230 0.79 +0.3 375,823 - H281DRAFT_02230 0.80 +1.3 375,823 - H281DRAFT_02230 0.80 -0.9 375,823 - H281DRAFT_02230 0.80 +0.9 375,823 - H281DRAFT_02230 0.80 -0.2 375,842 - H281DRAFT_02230 0.84 +0.1 375,936 + +1.0 375,936 + -1.5 375,937 - +0.5 375,937 - +0.6 375,937 - +1.3 375,937 - +1.0 375,937 - +0.5 376,048 - H281DRAFT_02231 0.20 -1.1 376,244 + H281DRAFT_02231 0.51 -0.9 376,244 + H281DRAFT_02231 0.51 +0.4 376,522 + +0.2 376,522 + +1.1 376,522 + +1.0 376,523 - +1.1 376,523 - -0.7 376,524 + -0.6 376,525 - +0.1 376,574 + +1.0 376,749 + H281DRAFT_02232 0.17 +0.6 376,750 - H281DRAFT_02232 0.17 +1.3 376,756 + H281DRAFT_02232 0.18 +1.1 376,756 + H281DRAFT_02232 0.18 +0.4 376,756 + H281DRAFT_02232 0.18 +0.1 376,766 + H281DRAFT_02232 0.19 +0.5 376,767 - H281DRAFT_02232 0.19 +0.6 376,767 - H281DRAFT_02232 0.19 +0.9 376,767 - H281DRAFT_02232 0.19 +0.4 376,767 - H281DRAFT_02232 0.19 +0.7 376,799 + H281DRAFT_02232 0.24 +1.1 376,800 - H281DRAFT_02232 0.24 -0.3 376,822 + H281DRAFT_02232 0.27 +0.3 377,045 - H281DRAFT_02232 0.57 +0.6 377,173 + H281DRAFT_02232 0.75 +0.8 377,173 + +1.6 377,180 + H281DRAFT_02232 0.76 -1.1 377,235 + H281DRAFT_02232 0.83 +1.8 377,235 + H281DRAFT_02232 0.83 +0.6 377,236 - H281DRAFT_02232 0.83 +1.5 377,266 - H281DRAFT_02232 0.88 +0.7 377,460 + +1.8 377,460 + -0.6 377,461 - -0.8 377,461 - +0.0 377,461 - -0.3 377,462 + +0.1 377,463 - -0.3 377,499 + +2.3 377,499 + +1.4 377,500 - -0.2 377,500 - +1.1 377,500 - -0.4 377,500 - +0.8 377,501 + +1.1 377,502 - +0.2 377,614 + +0.2 377,653 + +0.7 377,653 + -0.2 377,654 - +1.3 377,656 + -0.1 377,657 - +0.1 377,657 - -0.2
Or see this region's nucleotide sequence