Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_02165

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_02164 and H281DRAFT_02165 are separated by 111 nucleotidesH281DRAFT_02165 and H281DRAFT_02166 are separated by 274 nucleotidesH281DRAFT_02166 and H281DRAFT_02167 are separated by 155 nucleotides H281DRAFT_02164: H281DRAFT_02164 - carboxymethylenebutenolidase, at 303,513 to 304,211 _02164 H281DRAFT_02165: H281DRAFT_02165 - amidase, at 304,323 to 305,957 _02165 H281DRAFT_02166: H281DRAFT_02166 - High potential iron-sulfur protein, at 306,232 to 306,543 _02166 H281DRAFT_02167: H281DRAFT_02167 - L-2-hydroxyglutarate oxidase LhgO, at 306,699 to 307,805 _02167 Position (kb) 304 305 306Strain fitness (log2 ratio) -2 -1 0 1 2at 303.500 kb on + strandat 303.645 kb on - strand, within H281DRAFT_02164at 303.645 kb on - strand, within H281DRAFT_02164at 303.645 kb on - strand, within H281DRAFT_02164at 303.666 kb on + strand, within H281DRAFT_02164at 303.681 kb on + strand, within H281DRAFT_02164at 303.681 kb on + strand, within H281DRAFT_02164at 303.681 kb on + strand, within H281DRAFT_02164at 303.682 kb on - strand, within H281DRAFT_02164at 303.683 kb on + strand, within H281DRAFT_02164at 303.683 kb on + strand, within H281DRAFT_02164at 303.684 kb on - strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strandat 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.738 kb on + strand, within H281DRAFT_02164at 303.739 kb on - strand, within H281DRAFT_02164at 303.739 kb on - strand, within H281DRAFT_02164at 303.739 kb on - strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.900 kb on + strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strandat 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.901 kb on - strand, within H281DRAFT_02164at 303.902 kb on + strand, within H281DRAFT_02164at 303.902 kb on + strand, within H281DRAFT_02164at 303.903 kb on - strand, within H281DRAFT_02164at 303.903 kb on - strand, within H281DRAFT_02164at 303.943 kb on - strand, within H281DRAFT_02164at 303.946 kb on - strand, within H281DRAFT_02164at 304.012 kb on - strand, within H281DRAFT_02164at 304.101 kb on + strand, within H281DRAFT_02164at 304.102 kb on - strand, within H281DRAFT_02164at 304.146 kb on + strandat 304.147 kb on - strandat 304.149 kb on - strandat 304.209 kb on + strandat 304.209 kb on + strandat 304.380 kb on - strandat 304.424 kb on - strandat 304.431 kb on + strandat 304.431 kb on + strandat 304.431 kb on + strandat 304.432 kb on - strandat 304.566 kb on + strand, within H281DRAFT_02165at 304.567 kb on - strand, within H281DRAFT_02165at 304.567 kb on - strand, within H281DRAFT_02165at 304.689 kb on + strand, within H281DRAFT_02165at 304.860 kb on + strand, within H281DRAFT_02165at 304.860 kb on + strand, within H281DRAFT_02165at 304.862 kb on + strand, within H281DRAFT_02165at 304.862 kb on + strand, within H281DRAFT_02165at 304.862 kb on + strand, within H281DRAFT_02165at 304.863 kb on - strand, within H281DRAFT_02165at 304.863 kb on - strand, within H281DRAFT_02165at 304.863 kb on - strand, within H281DRAFT_02165at 304.913 kb on + strand, within H281DRAFT_02165at 304.914 kb on - strand, within H281DRAFT_02165at 304.914 kb on - strand, within H281DRAFT_02165at 305.241 kb on + strand, within H281DRAFT_02165at 305.241 kb on + strand, within H281DRAFT_02165at 305.241 kb on + strand, within H281DRAFT_02165at 305.241 kb on + strand, within H281DRAFT_02165at 305.241 kb on + strand, within H281DRAFT_02165at 305.242 kb on - strand, within H281DRAFT_02165at 305.242 kb on - strand, within H281DRAFT_02165at 305.242 kb on - strand, within H281DRAFT_02165at 305.334 kb on - strand, within H281DRAFT_02165at 305.738 kb on + strand, within H281DRAFT_02165at 305.739 kb on - strand, within H281DRAFT_02165at 305.812 kb on - strandat 305.812 kb on - strandat 305.939 kb on + strandat 305.939 kb on + strandat 305.955 kb on + strandat 305.955 kb on + strandat 305.955 kb on + strandat 305.956 kb on - strandat 305.990 kb on - strandat 305.990 kb on - strandat 305.990 kb on - strandat 305.990 kb on - strandat 305.990 kb on - strandat 305.993 kb on + strandat 305.993 kb on + strandat 305.993 kb on + strandat 305.993 kb on + strandat 305.994 kb on - strandat 305.994 kb on - strandat 305.994 kb on - strandat 305.994 kb on - strandat 306.090 kb on + strandat 306.091 kb on - strandat 306.180 kb on + strandat 306.180 kb on + strandat 306.246 kb on + strandat 306.247 kb on - strandat 306.248 kb on + strandat 306.248 kb on + strandat 306.248 kb on + strandat 306.249 kb on - strandat 306.249 kb on - strandat 306.249 kb on - strandat 306.249 kb on - strandat 306.249 kb on - strandat 306.309 kb on - strand, within H281DRAFT_02166at 306.333 kb on - strand, within H281DRAFT_02166at 306.368 kb on + strand, within H281DRAFT_02166at 306.369 kb on - strand, within H281DRAFT_02166at 306.369 kb on - strand, within H281DRAFT_02166at 306.413 kb on + strand, within H281DRAFT_02166at 306.508 kb on - strand, within H281DRAFT_02166at 306.560 kb on + strandat 306.560 kb on + strandat 306.560 kb on + strandat 306.561 kb on - strandat 306.562 kb on + strandat 306.562 kb on + strandat 306.562 kb on + strandat 306.563 kb on - strandat 306.563 kb on - strandat 306.604 kb on + strandat 306.604 kb on + strandat 306.605 kb on - strandat 306.621 kb on + strandat 306.621 kb on + strandat 306.621 kb on + strandat 306.621 kb on + strandat 306.641 kb on - strandat 306.646 kb on + strandat 306.646 kb on + strandat 306.647 kb on - strandat 306.647 kb on - strandat 306.877 kb on + strand, within H281DRAFT_02167at 306.877 kb on + strand, within H281DRAFT_02167at 306.934 kb on + strand, within H281DRAFT_02167

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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303,500 + +0.1
303,645 - H281DRAFT_02164 0.19 +0.1
303,645 - H281DRAFT_02164 0.19 -1.8
303,645 - H281DRAFT_02164 0.19 -0.5
303,666 + H281DRAFT_02164 0.22 +0.6
303,681 + H281DRAFT_02164 0.24 +0.3
303,681 + H281DRAFT_02164 0.24 +0.0
303,681 + H281DRAFT_02164 0.24 +0.6
303,682 - H281DRAFT_02164 0.24 +1.0
303,683 + H281DRAFT_02164 0.24 -1.0
303,683 + H281DRAFT_02164 0.24 +0.9
303,684 - H281DRAFT_02164 0.24 -0.5
303,738 + H281DRAFT_02164 0.32 +1.6
303,738 + H281DRAFT_02164 0.32 +0.1
303,738 + H281DRAFT_02164 0.32 +0.3
303,738 + H281DRAFT_02164 0.32 +0.4
303,738 + +0.5
303,738 + H281DRAFT_02164 0.32 -0.9
303,738 + H281DRAFT_02164 0.32 -0.3
303,738 + H281DRAFT_02164 0.32 -0.7
303,739 - H281DRAFT_02164 0.32 -0.7
303,739 - H281DRAFT_02164 0.32 -0.3
303,739 - H281DRAFT_02164 0.32 -0.2
303,900 + H281DRAFT_02164 0.55 +0.5
303,900 + H281DRAFT_02164 0.55 +0.3
303,900 + H281DRAFT_02164 0.55 +0.4
303,900 + H281DRAFT_02164 0.55 +0.5
303,900 + H281DRAFT_02164 0.55 +0.5
303,900 + H281DRAFT_02164 0.55 -0.4
303,900 + H281DRAFT_02164 0.55 +0.4
303,900 + H281DRAFT_02164 0.55 -0.0
303,900 + H281DRAFT_02164 0.55 -0.6
303,900 + H281DRAFT_02164 0.55 +0.2
303,900 + H281DRAFT_02164 0.55 -0.1
303,900 + H281DRAFT_02164 0.55 -0.2
303,900 + H281DRAFT_02164 0.55 -0.1
303,901 - H281DRAFT_02164 0.56 +2.0
303,901 - H281DRAFT_02164 0.56 -0.0
303,901 - H281DRAFT_02164 0.56 -1.1
303,901 - H281DRAFT_02164 0.56 +0.2
303,901 - H281DRAFT_02164 0.56 +0.1
303,901 - H281DRAFT_02164 0.56 +0.4
303,901 - H281DRAFT_02164 0.56 +0.1
303,901 - H281DRAFT_02164 0.56 -0.7
303,901 - H281DRAFT_02164 0.56 +1.0
303,901 - +2.4
303,901 - H281DRAFT_02164 0.56 -0.1
303,901 - H281DRAFT_02164 0.56 -0.2
303,901 - H281DRAFT_02164 0.56 +0.7
303,901 - H281DRAFT_02164 0.56 +0.7
303,901 - H281DRAFT_02164 0.56 +0.3
303,901 - H281DRAFT_02164 0.56 +0.2
303,902 + H281DRAFT_02164 0.56 -0.0
303,902 + H281DRAFT_02164 0.56 -0.3
303,903 - H281DRAFT_02164 0.56 +0.7
303,903 - H281DRAFT_02164 0.56 -2.0
303,943 - H281DRAFT_02164 0.62 +0.1
303,946 - H281DRAFT_02164 0.62 +0.2
304,012 - H281DRAFT_02164 0.71 +0.2
304,101 + H281DRAFT_02164 0.84 +0.0
304,102 - H281DRAFT_02164 0.84 -0.5
304,146 + -0.9
304,147 - -0.5
304,149 - -0.2
304,209 + +0.3
304,209 + +0.6
304,380 - -0.2
304,424 - +0.5
304,431 + +0.2
304,431 + +0.3
304,431 + -0.1
304,432 - -0.4
304,566 + H281DRAFT_02165 0.15 -0.7
304,567 - H281DRAFT_02165 0.15 -0.5
304,567 - H281DRAFT_02165 0.15 -0.4
304,689 + H281DRAFT_02165 0.22 +0.4
304,860 + H281DRAFT_02165 0.33 +0.6
304,860 + H281DRAFT_02165 0.33 +1.3
304,862 + H281DRAFT_02165 0.33 +0.8
304,862 + H281DRAFT_02165 0.33 -0.5
304,862 + H281DRAFT_02165 0.33 +0.3
304,863 - H281DRAFT_02165 0.33 -0.3
304,863 - H281DRAFT_02165 0.33 +0.1
304,863 - H281DRAFT_02165 0.33 -0.5
304,913 + H281DRAFT_02165 0.36 -0.5
304,914 - H281DRAFT_02165 0.36 +0.3
304,914 - H281DRAFT_02165 0.36 +0.3
305,241 + H281DRAFT_02165 0.56 +0.4
305,241 + H281DRAFT_02165 0.56 -0.2
305,241 + H281DRAFT_02165 0.56 +0.4
305,241 + H281DRAFT_02165 0.56 +0.2
305,241 + H281DRAFT_02165 0.56 +0.4
305,242 - H281DRAFT_02165 0.56 +1.3
305,242 - H281DRAFT_02165 0.56 +0.4
305,242 - H281DRAFT_02165 0.56 -0.5
305,334 - H281DRAFT_02165 0.62 +0.7
305,738 + H281DRAFT_02165 0.87 +0.4
305,739 - H281DRAFT_02165 0.87 +0.7
305,812 - +0.1
305,812 - +0.1
305,939 + -0.2
305,939 + +0.9
305,955 + -0.1
305,955 + +0.1
305,955 + +0.2
305,956 - -0.2
305,990 - -0.3
305,990 - -1.0
305,990 - +0.3
305,990 - +2.0
305,990 - -0.2
305,993 + +0.1
305,993 + +0.4
305,993 + +0.1
305,993 + -1.4
305,994 - -1.7
305,994 - -0.1
305,994 - -0.2
305,994 - -0.4
306,090 + +0.2
306,091 - +0.5
306,180 + -0.5
306,180 + +0.4
306,246 + +0.9
306,247 - +0.2
306,248 + -0.5
306,248 + +1.0
306,248 + +0.3
306,249 - +0.1
306,249 - +1.0
306,249 - +0.0
306,249 - +0.6
306,249 - +1.8
306,309 - H281DRAFT_02166 0.25 +0.8
306,333 - H281DRAFT_02166 0.32 +0.8
306,368 + H281DRAFT_02166 0.44 +0.3
306,369 - H281DRAFT_02166 0.44 -0.1
306,369 - H281DRAFT_02166 0.44 +0.8
306,413 + H281DRAFT_02166 0.58 +0.5
306,508 - H281DRAFT_02166 0.88 +0.3
306,560 + -0.3
306,560 + +0.5
306,560 + +0.2
306,561 - +0.4
306,562 + +1.2
306,562 + +1.2
306,562 + +0.2
306,563 - +0.1
306,563 - +1.3
306,604 + +0.4
306,604 + +0.5
306,605 - +1.3
306,621 + +0.1
306,621 + -0.2
306,621 + +0.5
306,621 + -1.3
306,641 - +0.4
306,646 + +0.0
306,646 + +0.3
306,647 - +0.1
306,647 - -0.2
306,877 + H281DRAFT_02167 0.16 -0.6
306,877 + H281DRAFT_02167 0.16 -0.0
306,934 + H281DRAFT_02167 0.21 +0.2

Or see this region's nucleotide sequence