Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02052 and H281DRAFT_02053 overlap by 29 nucleotides H281DRAFT_02053 and H281DRAFT_02054 are separated by 12 nucleotides H281DRAFT_02054 and H281DRAFT_02055 are separated by 334 nucleotides H281DRAFT_02055 and H281DRAFT_02056 are separated by 8 nucleotides
H281DRAFT_02052: H281DRAFT_02052 - hypothetical protein, at 175,682 to 175,951
_02052
H281DRAFT_02053: H281DRAFT_02053 - D-alanine transaminase, at 175,923 to 176,873
_02053
H281DRAFT_02054: H281DRAFT_02054 - penicillin-binding protein 6. Serine peptidase. MEROPS family S11, at 176,886 to 178,115
_02054
H281DRAFT_02055: H281DRAFT_02055 - hypothetical protein, at 178,450 to 179,094
_02055
H281DRAFT_02056: H281DRAFT_02056 - (2Fe-2S) ferredoxin, at 179,103 to 179,426
_02056
Position (kb)
176
177
178
179 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 175.920 kb on + strand, within H281DRAFT_02052 at 175.928 kb on + strand at 175.929 kb on - strand at 175.996 kb on + strand at 175.997 kb on - strand at 175.997 kb on - strand at 175.997 kb on - strand at 176.005 kb on + strand at 176.006 kb on - strand at 176.241 kb on + strand, within H281DRAFT_02053 at 176.241 kb on + strand, within H281DRAFT_02053 at 176.242 kb on - strand, within H281DRAFT_02053 at 176.242 kb on - strand, within H281DRAFT_02053 at 176.242 kb on - strand, within H281DRAFT_02053 at 176.242 kb on - strand, within H281DRAFT_02053 at 176.339 kb on - strand, within H281DRAFT_02053 at 176.339 kb on - strand, within H281DRAFT_02053 at 176.548 kb on + strand, within H281DRAFT_02053 at 176.549 kb on - strand, within H281DRAFT_02053 at 176.734 kb on + strand, within H281DRAFT_02053 at 176.735 kb on - strand, within H281DRAFT_02053 at 176.736 kb on + strand, within H281DRAFT_02053 at 176.737 kb on - strand, within H281DRAFT_02053 at 176.752 kb on + strand, within H281DRAFT_02053 at 176.753 kb on - strand, within H281DRAFT_02053 at 176.834 kb on - strand at 177.115 kb on + strand at 177.115 kb on + strand, within H281DRAFT_02054 at 177.116 kb on - strand, within H281DRAFT_02054 at 177.116 kb on - strand, within H281DRAFT_02054 at 177.199 kb on + strand, within H281DRAFT_02054 at 177.200 kb on - strand, within H281DRAFT_02054 at 177.200 kb on - strand, within H281DRAFT_02054 at 177.227 kb on - strand, within H281DRAFT_02054 at 177.232 kb on + strand, within H281DRAFT_02054 at 177.232 kb on + strand, within H281DRAFT_02054 at 177.232 kb on + strand, within H281DRAFT_02054 at 177.233 kb on - strand, within H281DRAFT_02054 at 177.233 kb on - strand, within H281DRAFT_02054 at 177.233 kb on - strand, within H281DRAFT_02054 at 177.454 kb on + strand, within H281DRAFT_02054 at 177.454 kb on + strand, within H281DRAFT_02054 at 177.455 kb on - strand, within H281DRAFT_02054 at 177.455 kb on - strand, within H281DRAFT_02054 at 177.484 kb on + strand, within H281DRAFT_02054 at 177.484 kb on + strand, within H281DRAFT_02054 at 177.484 kb on + strand, within H281DRAFT_02054 at 177.485 kb on - strand, within H281DRAFT_02054 at 177.485 kb on - strand, within H281DRAFT_02054 at 177.487 kb on + strand, within H281DRAFT_02054 at 177.547 kb on + strand, within H281DRAFT_02054 at 177.547 kb on + strand, within H281DRAFT_02054 at 177.548 kb on - strand, within H281DRAFT_02054 at 177.548 kb on - strand, within H281DRAFT_02054 at 177.548 kb on - strand, within H281DRAFT_02054 at 177.712 kb on + strand, within H281DRAFT_02054 at 177.856 kb on + strand, within H281DRAFT_02054 at 177.856 kb on + strand, within H281DRAFT_02054 at 178.051 kb on - strand at 178.116 kb on - strand at 178.116 kb on - strand at 178.134 kb on + strand at 178.134 kb on + strand at 178.134 kb on + strand at 178.135 kb on - strand at 178.135 kb on - strand at 178.135 kb on - strand at 178.136 kb on + strand at 178.201 kb on - strand at 178.265 kb on + strand at 178.298 kb on - strand at 178.353 kb on + strand at 178.353 kb on + strand at 178.355 kb on + strand at 178.355 kb on + strand at 178.355 kb on + strand at 178.355 kb on + strand at 178.355 kb on + strand at 178.356 kb on - strand at 178.356 kb on - strand at 178.364 kb on + strand at 178.415 kb on - strand at 178.420 kb on + strand at 178.485 kb on + strand at 178.883 kb on + strand, within H281DRAFT_02055 at 178.883 kb on + strand, within H281DRAFT_02055 at 178.884 kb on - strand, within H281DRAFT_02055 at 178.884 kb on - strand, within H281DRAFT_02055 at 178.884 kb on - strand, within H281DRAFT_02055 at 178.892 kb on + strand, within H281DRAFT_02055 at 179.072 kb on + strand at 179.072 kb on - strand at 179.082 kb on + strand at 179.082 kb on + strand at 179.082 kb on + strand at 179.083 kb on - strand at 179.083 kb on - strand at 179.104 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 175,920 + H281DRAFT_02052 0.88 -0.1 175,928 + +1.0 175,929 - +0.2 175,996 + -1.8 175,997 - -0.8 175,997 - -0.0 175,997 - +0.2 176,005 + -0.2 176,006 - -0.2 176,241 + H281DRAFT_02053 0.33 -0.1 176,241 + H281DRAFT_02053 0.33 -0.6 176,242 - H281DRAFT_02053 0.34 +0.7 176,242 - H281DRAFT_02053 0.34 -0.7 176,242 - H281DRAFT_02053 0.34 +0.3 176,242 - H281DRAFT_02053 0.34 -0.2 176,339 - H281DRAFT_02053 0.44 +0.0 176,339 - H281DRAFT_02053 0.44 +0.1 176,548 + H281DRAFT_02053 0.66 -0.6 176,549 - H281DRAFT_02053 0.66 -0.2 176,734 + H281DRAFT_02053 0.85 +1.0 176,735 - H281DRAFT_02053 0.85 +0.3 176,736 + H281DRAFT_02053 0.85 +0.3 176,737 - H281DRAFT_02053 0.86 +0.9 176,752 + H281DRAFT_02053 0.87 +0.7 176,753 - H281DRAFT_02053 0.87 +0.1 176,834 - +0.3 177,115 + +1.0 177,115 + H281DRAFT_02054 0.19 +0.5 177,116 - H281DRAFT_02054 0.19 +0.8 177,116 - H281DRAFT_02054 0.19 +2.2 177,199 + H281DRAFT_02054 0.25 +2.7 177,200 - H281DRAFT_02054 0.26 +0.1 177,200 - H281DRAFT_02054 0.26 +1.6 177,227 - H281DRAFT_02054 0.28 +1.0 177,232 + H281DRAFT_02054 0.28 +2.3 177,232 + H281DRAFT_02054 0.28 -1.3 177,232 + H281DRAFT_02054 0.28 +1.1 177,233 - H281DRAFT_02054 0.28 -0.2 177,233 - H281DRAFT_02054 0.28 +0.3 177,233 - H281DRAFT_02054 0.28 +0.0 177,454 + H281DRAFT_02054 0.46 -0.3 177,454 + H281DRAFT_02054 0.46 +0.8 177,455 - H281DRAFT_02054 0.46 +0.9 177,455 - H281DRAFT_02054 0.46 -0.1 177,484 + H281DRAFT_02054 0.49 +0.6 177,484 + H281DRAFT_02054 0.49 +0.1 177,484 + H281DRAFT_02054 0.49 -0.1 177,485 - H281DRAFT_02054 0.49 +0.1 177,485 - H281DRAFT_02054 0.49 +1.0 177,487 + H281DRAFT_02054 0.49 +0.9 177,547 + H281DRAFT_02054 0.54 -1.6 177,547 + H281DRAFT_02054 0.54 +1.6 177,548 - H281DRAFT_02054 0.54 +0.1 177,548 - H281DRAFT_02054 0.54 +0.4 177,548 - H281DRAFT_02054 0.54 -0.3 177,712 + H281DRAFT_02054 0.67 +0.2 177,856 + H281DRAFT_02054 0.79 -0.5 177,856 + H281DRAFT_02054 0.79 +0.5 178,051 - +0.1 178,116 - +0.8 178,116 - +1.0 178,134 + -1.1 178,134 + -0.3 178,134 + +0.2 178,135 - +0.4 178,135 - -0.3 178,135 - +0.4 178,136 + +0.9 178,201 - -1.9 178,265 + +0.3 178,298 - +0.4 178,353 + +0.6 178,353 + -0.1 178,355 + -0.1 178,355 + +0.9 178,355 + -0.2 178,355 + -0.2 178,355 + +0.2 178,356 - +0.1 178,356 - -1.3 178,364 + +1.0 178,415 - -0.0 178,420 + -0.0 178,485 + +1.0 178,883 + H281DRAFT_02055 0.67 -0.6 178,883 + H281DRAFT_02055 0.67 +0.9 178,884 - H281DRAFT_02055 0.67 -0.3 178,884 - H281DRAFT_02055 0.67 -0.7 178,884 - H281DRAFT_02055 0.67 -0.1 178,892 + H281DRAFT_02055 0.69 -0.8 179,072 + -0.8 179,072 - -0.4 179,082 + -0.1 179,082 + -0.8 179,082 + -1.0 179,083 - +0.7 179,083 - -0.0 179,104 + -0.1
Or see this region's nucleotide sequence