Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02001 and H281DRAFT_02002 are separated by 150 nucleotides H281DRAFT_02002 and H281DRAFT_02003 overlap by 4 nucleotides H281DRAFT_02003 and H281DRAFT_02004 are separated by 22 nucleotides H281DRAFT_02004 and H281DRAFT_02005 are separated by 378 nucleotides
H281DRAFT_02001: H281DRAFT_02001 - protein translocase subunit secB, at 118,871 to 119,341
_02001
H281DRAFT_02002: H281DRAFT_02002 - glutaredoxin 3, at 119,492 to 119,752
_02002
H281DRAFT_02003: H281DRAFT_02003 - Rhodanese-related sulfurtransferase, at 119,749 to 120,222
_02003
H281DRAFT_02004: H281DRAFT_02004 - phosphoglycerate mutase, at 120,245 to 121,057
_02004
H281DRAFT_02005: H281DRAFT_02005 - carboxyl-terminal processing protease, at 121,436 to 123,013
_02005
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 119.360 kb on + strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.364 kb on + strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.370 kb on + strand at 119.403 kb on + strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.453 kb on - strand at 119.453 kb on - strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.734 kb on - strand at 119.759 kb on - strand at 119.853 kb on + strand, within H281DRAFT_02003 at 119.913 kb on + strand, within H281DRAFT_02003 at 119.913 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.155 kb on + strand, within H281DRAFT_02003 at 120.156 kb on - strand, within H281DRAFT_02003 at 120.223 kb on + strand at 120.224 kb on - strand at 120.225 kb on + strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.226 kb on - strand at 120.230 kb on + strand at 120.231 kb on - strand at 120.231 kb on - strand at 120.260 kb on + strand at 120.260 kb on + strand at 120.260 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.310 kb on + strand at 120.311 kb on - strand at 120.314 kb on + strand at 120.315 kb on - strand at 120.453 kb on - strand, within H281DRAFT_02004 at 120.470 kb on + strand, within H281DRAFT_02004 at 120.472 kb on + strand, within H281DRAFT_02004 at 120.473 kb on - strand, within H281DRAFT_02004 at 120.473 kb on - strand, within H281DRAFT_02004 at 120.536 kb on + strand, within H281DRAFT_02004 at 120.537 kb on - strand, within H281DRAFT_02004 at 120.537 kb on - strand, within H281DRAFT_02004 at 120.537 kb on - strand, within H281DRAFT_02004 at 120.884 kb on + strand, within H281DRAFT_02004 at 120.951 kb on - strand, within H281DRAFT_02004 at 120.951 kb on - strand, within H281DRAFT_02004 at 120.987 kb on - strand at 120.989 kb on + strand at 120.990 kb on - strand at 121.083 kb on + strand at 121.083 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.094 kb on + strand at 121.095 kb on - strand at 121.095 kb on - strand at 121.095 kb on - strand at 121.095 kb on - strand at 121.277 kb on + strand at 121.278 kb on - strand at 121.278 kb on - strand at 121.278 kb on - strand at 121.278 kb on - strand at 121.278 kb on - strand at 121.375 kb on + strand at 121.375 kb on + strand at 121.375 kb on + strand at 121.376 kb on - strand at 121.377 kb on + strand at 121.377 kb on + strand at 121.377 kb on + strand at 121.377 kb on + strand at 121.397 kb on + strand at 121.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 119,360 + -2.4 119,361 - +0.7 119,361 - -0.4 119,361 - -0.5 119,361 - -0.8 119,361 - +0.2 119,364 + -1.3 119,365 - +0.3 119,365 - +0.1 119,365 - +0.1 119,365 - -0.6 119,365 - +0.6 119,365 - -0.1 119,365 - -0.9 119,365 - +0.0 119,370 + +1.0 119,403 + -0.6 119,404 - -0.4 119,404 - +0.4 119,404 - -0.4 119,404 - +0.4 119,404 - +0.4 119,453 - +0.6 119,453 - -0.1 119,733 + -1.5 119,733 + +0.0 119,733 + -1.7 119,733 + -0.8 119,734 - -1.6 119,759 - +1.9 119,853 + H281DRAFT_02003 0.22 +0.7 119,913 + H281DRAFT_02003 0.35 -0.4 119,913 + H281DRAFT_02003 0.35 +0.4 120,155 + H281DRAFT_02003 0.86 +1.2 120,155 + H281DRAFT_02003 0.86 +1.4 120,155 + H281DRAFT_02003 0.86 +0.3 120,156 - H281DRAFT_02003 0.86 +1.0 120,223 + +0.4 120,224 - +0.3 120,225 + -0.1 120,226 - -0.2 120,226 - -0.8 120,226 - -0.1 120,226 - +1.2 120,230 + +0.2 120,231 - +1.2 120,231 - -1.9 120,260 + -0.5 120,260 + -1.2 120,260 + -0.3 120,310 + -0.6 120,310 + -2.6 120,310 + -0.6 120,310 + +0.5 120,310 + +0.4 120,310 + +0.6 120,311 - -2.7 120,314 + -2.4 120,315 - +0.0 120,453 - H281DRAFT_02004 0.26 -0.9 120,470 + H281DRAFT_02004 0.28 +0.7 120,472 + H281DRAFT_02004 0.28 -1.3 120,473 - H281DRAFT_02004 0.28 -3.0 120,473 - H281DRAFT_02004 0.28 +0.0 120,536 + H281DRAFT_02004 0.36 -2.7 120,537 - H281DRAFT_02004 0.36 -0.7 120,537 - H281DRAFT_02004 0.36 -1.5 120,537 - H281DRAFT_02004 0.36 -1.4 120,884 + H281DRAFT_02004 0.79 +0.0 120,951 - H281DRAFT_02004 0.87 -1.2 120,951 - H281DRAFT_02004 0.87 -2.2 120,987 - -1.7 120,989 + -2.8 120,990 - +0.0 121,083 + +0.2 121,083 + +0.6 121,094 + +0.4 121,094 + +0.2 121,094 + +0.3 121,094 + -0.0 121,094 + -0.3 121,094 + +0.3 121,094 + -0.5 121,095 - -0.4 121,095 - +1.0 121,095 - -0.1 121,095 - +0.0 121,277 + -0.1 121,278 - +0.1 121,278 - -0.1 121,278 - +0.2 121,278 - +0.2 121,278 - +1.8 121,375 + -0.4 121,375 + +1.0 121,375 + -0.0 121,376 - +0.0 121,377 + +0.4 121,377 + -1.8 121,377 + -0.1 121,377 + -2.4 121,397 + +0.7 121,435 + +0.1
Or see this region's nucleotide sequence