Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01889

Experiment: 2'-Deoxyinosine 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01887 and H281DRAFT_01888 are separated by 22 nucleotidesH281DRAFT_01888 and H281DRAFT_01889 overlap by 4 nucleotidesH281DRAFT_01889 and H281DRAFT_01890 are separated by 56 nucleotidesH281DRAFT_01890 and H281DRAFT_01891 are separated by 73 nucleotides H281DRAFT_01887: H281DRAFT_01887 - threonine ammonia-lyase, biosynthetic, long form, at 1 to 846 _01887 H281DRAFT_01888: H281DRAFT_01888 - 5'-nucleotidase, at 869 to 1,771 _01888 H281DRAFT_01889: H281DRAFT_01889 - 7-cyano-7-deazaguanine reductase, at 1,768 to 2,592 _01889 H281DRAFT_01890: H281DRAFT_01890 - reactive intermediate/imine deaminase, at 2,649 to 3,035 _01890 H281DRAFT_01891: H281DRAFT_01891 - N-acyl-D-amino-acid deacylase, at 3,109 to 4,590 _01891 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1 2 3at 0.827 kb on - strandat 0.827 kb on - strandat 0.898 kb on - strandat 0.951 kb on - strandat 0.951 kb on - strandat 0.966 kb on + strand, within H281DRAFT_01888at 0.966 kb on + strand, within H281DRAFT_01888at 0.966 kb on + strand, within H281DRAFT_01888at 0.974 kb on + strand, within H281DRAFT_01888at 0.974 kb on + strand, within H281DRAFT_01888at 0.975 kb on - strand, within H281DRAFT_01888at 0.975 kb on - strand, within H281DRAFT_01888at 1.237 kb on + strand, within H281DRAFT_01888at 1.318 kb on + strand, within H281DRAFT_01888at 1.318 kb on + strand, within H281DRAFT_01888at 1.319 kb on - strand, within H281DRAFT_01888at 1.366 kb on - strand, within H281DRAFT_01888at 1.438 kb on + strand, within H281DRAFT_01888at 1.439 kb on - strand, within H281DRAFT_01888at 1.532 kb on - strand, within H281DRAFT_01888at 1.532 kb on - strand, within H281DRAFT_01888at 1.537 kb on + strand, within H281DRAFT_01888at 1.808 kb on - strandat 1.819 kb on + strandat 1.921 kb on + strandat 1.922 kb on - strandat 2.029 kb on + strand, within H281DRAFT_01889at 2.100 kb on - strand, within H281DRAFT_01889at 2.221 kb on + strand, within H281DRAFT_01889at 2.392 kb on + strandat 2.393 kb on - strandat 2.409 kb on + strand, within H281DRAFT_01889at 2.495 kb on - strand, within H281DRAFT_01889at 2.506 kb on - strand, within H281DRAFT_01889at 2.548 kb on + strandat 2.548 kb on + strandat 2.549 kb on - strandat 2.591 kb on - strandat 2.591 kb on - strandat 2.668 kb on + strandat 2.668 kb on + strandat 2.669 kb on - strandat 2.669 kb on - strandat 2.862 kb on + strand, within H281DRAFT_01890at 2.862 kb on + strand, within H281DRAFT_01890at 2.863 kb on - strand, within H281DRAFT_01890at 2.863 kb on - strand, within H281DRAFT_01890at 2.987 kb on + strand, within H281DRAFT_01890at 2.988 kb on - strand, within H281DRAFT_01890at 2.988 kb on - strand, within H281DRAFT_01890at 3.048 kb on + strandat 3.048 kb on + strandat 3.049 kb on - strandat 3.089 kb on + strandat 3.089 kb on + strandat 3.089 kb on + strandat 3.090 kb on - strandat 3.220 kb on + strandat 3.221 kb on - strandat 3.423 kb on - strand, within H281DRAFT_01891

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Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
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827 - -0.5
827 - +1.0
898 - -0.3
951 - -0.0
951 - +0.1
966 + H281DRAFT_01888 0.11 -0.2
966 + H281DRAFT_01888 0.11 -0.4
966 + H281DRAFT_01888 0.11 +0.4
974 + H281DRAFT_01888 0.12 +0.1
974 + H281DRAFT_01888 0.12 -0.3
975 - H281DRAFT_01888 0.12 -0.8
975 - H281DRAFT_01888 0.12 +0.5
1,237 + H281DRAFT_01888 0.41 +0.7
1,318 + H281DRAFT_01888 0.50 -1.0
1,318 + H281DRAFT_01888 0.50 -0.5
1,319 - H281DRAFT_01888 0.50 -1.5
1,366 - H281DRAFT_01888 0.55 -0.5
1,438 + H281DRAFT_01888 0.63 +0.4
1,439 - H281DRAFT_01888 0.63 +0.5
1,532 - H281DRAFT_01888 0.73 -0.2
1,532 - H281DRAFT_01888 0.73 -0.7
1,537 + H281DRAFT_01888 0.74 +0.4
1,808 - +0.5
1,819 + +0.1
1,921 + +1.0
1,922 - +1.0
2,029 + H281DRAFT_01889 0.32 -0.9
2,100 - H281DRAFT_01889 0.40 -0.4
2,221 + H281DRAFT_01889 0.55 -1.3
2,392 + -0.6
2,393 - -0.6
2,409 + H281DRAFT_01889 0.78 +0.3
2,495 - H281DRAFT_01889 0.88 -0.8
2,506 - H281DRAFT_01889 0.89 -1.0
2,548 + -1.5
2,548 + +2.0
2,549 - -0.8
2,591 - -0.4
2,591 - -0.2
2,668 + -0.1
2,668 + -0.4
2,669 - -0.8
2,669 - +0.1
2,862 + H281DRAFT_01890 0.55 +0.5
2,862 + H281DRAFT_01890 0.55 +0.2
2,863 - H281DRAFT_01890 0.55 +0.2
2,863 - H281DRAFT_01890 0.55 +0.4
2,987 + H281DRAFT_01890 0.87 +0.4
2,988 - H281DRAFT_01890 0.88 -0.3
2,988 - H281DRAFT_01890 0.88 +3.3
3,048 + +1.0
3,048 + +0.2
3,049 - +0.0
3,089 + +0.3
3,089 + -0.2
3,089 + -0.3
3,090 - +1.3
3,220 + -0.4
3,221 - +0.7
3,423 - H281DRAFT_01891 0.21 -0.0

Or see this region's nucleotide sequence