Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01653 and H281DRAFT_01654 overlap by 4 nucleotides H281DRAFT_01654 and H281DRAFT_01655 are separated by 66 nucleotides H281DRAFT_01655 and H281DRAFT_01656 are separated by 70 nucleotides H281DRAFT_01656 and H281DRAFT_01657 are separated by 4 nucleotides H281DRAFT_01657 and H281DRAFT_01658 overlap by 4 nucleotides
H281DRAFT_01653: H281DRAFT_01653 - Phenylpropionate dioxygenase, large terminal subunit, at 535,490 to 536,773
_01653
H281DRAFT_01654: H281DRAFT_01654 - 3-phenylpropionate/cinnamic acid dioxygenase, small subunit, at 536,770 to 537,270
_01654
H281DRAFT_01655: H281DRAFT_01655 - 4-oxalocrotonate tautomerase family enzyme, at 537,337 to 537,711
_01655
H281DRAFT_01656: H281DRAFT_01656 - hypothetical protein, at 537,782 to 538,159
_01656
H281DRAFT_01657: H281DRAFT_01657 - SnoaL-like domain-containing protein, at 538,164 to 538,679
_01657
H281DRAFT_01658: H281DRAFT_01658 - amidase, at 538,676 to 539,818
_01658
Position (kb) 537
538 Strain fitness (log2 ratio) -1
0
1 at 536.361 kb on + strand, within H281DRAFT_01653 at 536.362 kb on - strand, within H281DRAFT_01653 at 536.624 kb on + strand, within H281DRAFT_01653 at 536.624 kb on + strand, within H281DRAFT_01653 at 536.866 kb on + strand, within H281DRAFT_01654 at 536.938 kb on + strand, within H281DRAFT_01654 at 536.956 kb on + strand, within H281DRAFT_01654 at 537.729 kb on - strand at 537.767 kb on + strand at 537.791 kb on - strand at 537.927 kb on + strand, within H281DRAFT_01656 at 537.998 kb on + strand, within H281DRAFT_01656 at 538.076 kb on + strand at 538.077 kb on - strand, within H281DRAFT_01656 at 538.248 kb on + strand, within H281DRAFT_01657 at 538.249 kb on - strand, within H281DRAFT_01657 at 538.522 kb on - strand, within H281DRAFT_01657
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 536,361 + H281DRAFT_01653 0.68 +0.2 536,362 - H281DRAFT_01653 0.68 -0.1 536,624 + H281DRAFT_01653 0.88 +0.8 536,624 + H281DRAFT_01653 0.88 -0.1 536,866 + H281DRAFT_01654 0.19 -0.2 536,938 + H281DRAFT_01654 0.34 -0.2 536,956 + H281DRAFT_01654 0.37 -0.4 537,729 - -0.4 537,767 + -0.1 537,791 - +0.1 537,927 + H281DRAFT_01656 0.38 +1.2 537,998 + H281DRAFT_01656 0.57 -0.5 538,076 + -0.5 538,077 - H281DRAFT_01656 0.78 +0.3 538,248 + H281DRAFT_01657 0.16 +0.3 538,249 - H281DRAFT_01657 0.16 +0.0 538,522 - H281DRAFT_01657 0.69 -1.2
Or see this region's nucleotide sequence