Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00482 and H281DRAFT_00483 are separated by 157 nucleotides H281DRAFT_00483 and H281DRAFT_00484 are separated by 15 nucleotides H281DRAFT_00484 and H281DRAFT_00485 are separated by 97 nucleotides H281DRAFT_00485 and H281DRAFT_00486 are separated by 235 nucleotides
H281DRAFT_00482: H281DRAFT_00482 - molecular chaperone HscA, at 539,384 to 541,255
_00482
H281DRAFT_00483: H281DRAFT_00483 - ferredoxin, 2Fe-2S, at 541,413 to 541,754
_00483
H281DRAFT_00484: H281DRAFT_00484 - FeS assembly protein IscX, at 541,770 to 541,967
_00484
H281DRAFT_00485: H281DRAFT_00485 - Glycine zipper 2TM domain-containing protein, at 542,065 to 542,829
_00485
H281DRAFT_00486: H281DRAFT_00486 - lysyl-tRNA synthetase, class II, at 543,065 to 544,675
_00486
Position (kb) 542
543 Strain fitness (log2 ratio) -1
0
1 at 541.753 kb on - strand at 541.753 kb on - strand at 541.963 kb on - strand at 542.135 kb on + strand at 542.183 kb on + strand, within H281DRAFT_00485 at 542.202 kb on + strand, within H281DRAFT_00485 at 542.430 kb on - strand, within H281DRAFT_00485 at 542.465 kb on + strand, within H281DRAFT_00485 at 542.860 kb on + strand at 542.860 kb on + strand at 542.861 kb on - strand at 542.861 kb on - strand at 542.862 kb on - strand at 542.909 kb on + strand at 542.960 kb on - strand at 542.969 kb on + strand at 542.970 kb on - strand at 543.060 kb on - strand at 543.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 541,753 - +1.1 541,753 - -0.3 541,963 - +1.4 542,135 + -0.3 542,183 + H281DRAFT_00485 0.15 -0.5 542,202 + H281DRAFT_00485 0.18 +0.6 542,430 - H281DRAFT_00485 0.48 -0.8 542,465 + H281DRAFT_00485 0.52 -0.4 542,860 + -1.2 542,860 + +1.0 542,861 - +0.2 542,861 - +0.3 542,862 - -0.3 542,909 + -0.1 542,960 - -0.5 542,969 + +0.5 542,970 - +0.1 543,060 - +0.3 543,060 - +0.7
Or see this region's nucleotide sequence