Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00463 and H281DRAFT_00464 are separated by 354 nucleotides H281DRAFT_00464 and H281DRAFT_00465 overlap by 4 nucleotides H281DRAFT_00465 and H281DRAFT_00466 are separated by 266 nucleotides
H281DRAFT_00463: H281DRAFT_00463 - methenyltetrahydrofolate cyclohydrolase /5,10-methylenetetrahydrofolate dehydrogenase (NADP+), at 516,525 to 517,385
_00463
H281DRAFT_00464: H281DRAFT_00464 - two component transcriptional regulator, LuxR family, at 517,740 to 518,387
_00464
H281DRAFT_00465: H281DRAFT_00465 - PAS/PAC sensor signal transduction histidine kinase /multi-sensor signal transduction histidine kinase, at 518,384 to 520,909
_00465
H281DRAFT_00466: H281DRAFT_00466 - pyruvate dehydrogenase E1 component, at 521,176 to 523,872
_00466
Position (kb) 518
519
520
521 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 517.499 kb on - strand at 517.524 kb on - strand at 517.525 kb on + strand at 517.526 kb on - strand at 517.623 kb on + strand at 517.741 kb on + strand at 519.077 kb on - strand, within H281DRAFT_00465 at 520.141 kb on - strand, within H281DRAFT_00465 at 520.141 kb on - strand, within H281DRAFT_00465 at 520.141 kb on - strand, within H281DRAFT_00465 at 520.222 kb on - strand, within H281DRAFT_00465 at 520.912 kb on + strand at 520.913 kb on - strand at 520.925 kb on - strand at 520.925 kb on - strand at 520.925 kb on - strand at 520.925 kb on - strand at 520.925 kb on - strand at 520.977 kb on + strand at 520.977 kb on + strand at 520.978 kb on - strand at 521.094 kb on + strand at 521.094 kb on + strand at 521.095 kb on - strand at 521.095 kb on - strand at 521.158 kb on + strand at 521.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 517,499 - -0.6 517,524 - -0.6 517,525 + +0.9 517,526 - -2.1 517,623 + -0.8 517,741 + +0.2 519,077 - H281DRAFT_00465 0.27 -1.9 520,141 - H281DRAFT_00465 0.70 -1.3 520,141 - H281DRAFT_00465 0.70 -4.4 520,141 - H281DRAFT_00465 0.70 +0.1 520,222 - H281DRAFT_00465 0.73 -2.6 520,912 + +0.7 520,913 - -1.1 520,925 - +0.3 520,925 - +0.2 520,925 - -1.0 520,925 - -1.2 520,925 - -0.6 520,977 + +0.1 520,977 + +0.2 520,978 - -0.9 521,094 + +0.1 521,094 + -0.5 521,095 - +0.6 521,095 - -1.6 521,158 + +1.7 521,160 + +0.5
Or see this region's nucleotide sequence