Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00298 and H281DRAFT_00299 are separated by 264 nucleotides H281DRAFT_00299 and H281DRAFT_00300 are separated by 275 nucleotides H281DRAFT_00300 and H281DRAFT_00301 are separated by 187 nucleotides
H281DRAFT_00298: H281DRAFT_00298 - hypothetical protein, at 319,339 to 319,584
_00298
H281DRAFT_00299: H281DRAFT_00299 - Protein of unknown function (DUF3096), at 319,849 to 320,001
_00299
H281DRAFT_00300: H281DRAFT_00300 - 6-phosphogluconolactonase, at 320,277 to 321,581
_00300
H281DRAFT_00301: H281DRAFT_00301 - DNA translocase FtsK, at 321,769 to 325,794
_00301
Position (kb)
320
321
322 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 319.522 kb on + strand, within H281DRAFT_00298 at 319.523 kb on - strand, within H281DRAFT_00298 at 319.523 kb on - strand, within H281DRAFT_00298 at 319.657 kb on + strand at 319.658 kb on - strand at 319.658 kb on - strand at 319.658 kb on - strand at 319.816 kb on + strand at 319.905 kb on - strand, within H281DRAFT_00299 at 319.917 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.919 kb on + strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.920 kb on - strand, within H281DRAFT_00299 at 319.951 kb on + strand, within H281DRAFT_00299 at 319.952 kb on - strand, within H281DRAFT_00299 at 320.005 kb on + strand at 320.006 kb on - strand at 320.012 kb on + strand at 320.024 kb on - strand at 320.114 kb on - strand at 320.179 kb on + strand at 320.179 kb on + strand at 320.180 kb on - strand at 320.181 kb on + strand at 320.181 kb on + strand at 320.182 kb on - strand at 320.182 kb on - strand at 320.194 kb on + strand at 320.194 kb on + strand at 320.195 kb on - strand at 320.237 kb on - strand at 320.237 kb on - strand at 320.237 kb on - strand at 320.256 kb on - strand at 320.264 kb on + strand at 320.274 kb on + strand at 320.275 kb on - strand at 320.276 kb on + strand at 320.276 kb on + strand at 320.276 kb on + strand at 320.276 kb on + strand at 320.276 kb on + strand at 320.277 kb on - strand at 320.277 kb on - strand at 320.277 kb on - strand at 320.277 kb on - strand at 320.277 kb on - strand at 320.277 kb on - strand at 320.321 kb on + strand at 320.322 kb on - strand at 320.338 kb on - strand at 320.408 kb on - strand, within H281DRAFT_00300 at 320.408 kb on - strand, within H281DRAFT_00300 at 320.491 kb on - strand, within H281DRAFT_00300 at 320.511 kb on + strand, within H281DRAFT_00300 at 320.511 kb on + strand, within H281DRAFT_00300 at 320.512 kb on - strand, within H281DRAFT_00300 at 320.512 kb on - strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.538 kb on + strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.539 kb on - strand, within H281DRAFT_00300 at 320.610 kb on + strand, within H281DRAFT_00300 at 320.611 kb on - strand, within H281DRAFT_00300 at 320.611 kb on - strand, within H281DRAFT_00300 at 320.643 kb on + strand, within H281DRAFT_00300 at 320.778 kb on + strand, within H281DRAFT_00300 at 320.778 kb on + strand, within H281DRAFT_00300 at 320.779 kb on - strand, within H281DRAFT_00300 at 320.805 kb on + strand, within H281DRAFT_00300 at 320.805 kb on + strand, within H281DRAFT_00300 at 320.805 kb on + strand, within H281DRAFT_00300 at 320.806 kb on - strand, within H281DRAFT_00300 at 320.992 kb on - strand, within H281DRAFT_00300 at 321.027 kb on + strand, within H281DRAFT_00300 at 321.027 kb on + strand, within H281DRAFT_00300 at 321.027 kb on + strand, within H281DRAFT_00300 at 321.027 kb on + strand, within H281DRAFT_00300 at 321.028 kb on - strand, within H281DRAFT_00300 at 321.028 kb on - strand, within H281DRAFT_00300 at 321.087 kb on + strand, within H281DRAFT_00300 at 321.087 kb on + strand, within H281DRAFT_00300 at 321.087 kb on + strand, within H281DRAFT_00300 at 321.088 kb on - strand, within H281DRAFT_00300 at 321.089 kb on + strand, within H281DRAFT_00300 at 321.090 kb on - strand, within H281DRAFT_00300 at 321.153 kb on + strand, within H281DRAFT_00300 at 321.195 kb on + strand, within H281DRAFT_00300 at 321.196 kb on - strand, within H281DRAFT_00300 at 321.307 kb on - strand, within H281DRAFT_00300 at 321.307 kb on - strand, within H281DRAFT_00300 at 321.307 kb on - strand, within H281DRAFT_00300 at 321.480 kb on + strand at 321.480 kb on + strand at 321.480 kb on + strand at 321.480 kb on + strand at 321.601 kb on - strand at 321.648 kb on + strand at 321.649 kb on - strand at 321.649 kb on - strand at 321.700 kb on + strand at 321.701 kb on - strand at 321.701 kb on - strand at 321.873 kb on + strand at 321.873 kb on + strand at 321.875 kb on + strand at 321.876 kb on - strand at 322.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 319,522 + H281DRAFT_00298 0.74 +2.0 319,523 - H281DRAFT_00298 0.75 +0.8 319,523 - H281DRAFT_00298 0.75 +1.5 319,657 + +0.4 319,658 - +0.2 319,658 - +0.5 319,658 - +0.7 319,816 + +0.2 319,905 - H281DRAFT_00299 0.37 +0.6 319,917 + H281DRAFT_00299 0.44 -0.7 319,919 + H281DRAFT_00299 0.46 -0.4 319,919 + H281DRAFT_00299 0.46 +0.3 319,919 + H281DRAFT_00299 0.46 -0.6 319,919 + H281DRAFT_00299 0.46 -0.0 319,920 - H281DRAFT_00299 0.46 -1.5 319,920 - H281DRAFT_00299 0.46 +0.7 319,920 - H281DRAFT_00299 0.46 +0.6 319,920 - H281DRAFT_00299 0.46 -0.5 319,920 - H281DRAFT_00299 0.46 -1.6 319,920 - H281DRAFT_00299 0.46 +0.2 319,951 + H281DRAFT_00299 0.67 -0.5 319,952 - H281DRAFT_00299 0.67 +0.3 320,005 + +0.2 320,006 - +0.1 320,012 + +0.8 320,024 - -0.3 320,114 - +0.4 320,179 + +0.4 320,179 + -0.2 320,180 - -0.1 320,181 + +0.4 320,181 + -0.0 320,182 - +1.0 320,182 - -0.1 320,194 + +1.0 320,194 + -1.1 320,195 - -0.1 320,237 - +0.1 320,237 - +0.3 320,237 - -0.2 320,256 - -0.6 320,264 + +0.2 320,274 + +0.1 320,275 - -0.2 320,276 + -0.0 320,276 + -0.6 320,276 + +0.6 320,276 + +1.5 320,276 + +0.7 320,277 - +0.3 320,277 - +0.3 320,277 - +0.2 320,277 - +0.1 320,277 - +1.0 320,277 - +0.0 320,321 + -0.2 320,322 - +0.5 320,338 - -0.2 320,408 - H281DRAFT_00300 0.10 +0.6 320,408 - H281DRAFT_00300 0.10 +1.2 320,491 - H281DRAFT_00300 0.16 +0.3 320,511 + H281DRAFT_00300 0.18 -0.0 320,511 + H281DRAFT_00300 0.18 +0.1 320,512 - H281DRAFT_00300 0.18 +0.3 320,512 - H281DRAFT_00300 0.18 +0.3 320,538 + H281DRAFT_00300 0.20 +1.3 320,538 + H281DRAFT_00300 0.20 +0.2 320,538 + H281DRAFT_00300 0.20 -1.3 320,538 + H281DRAFT_00300 0.20 -0.3 320,538 + H281DRAFT_00300 0.20 +0.4 320,538 + H281DRAFT_00300 0.20 -1.0 320,538 + H281DRAFT_00300 0.20 -0.5 320,538 + H281DRAFT_00300 0.20 -0.5 320,538 + H281DRAFT_00300 0.20 -0.9 320,538 + H281DRAFT_00300 0.20 +0.1 320,538 + H281DRAFT_00300 0.20 +0.3 320,539 - H281DRAFT_00300 0.20 +1.0 320,539 - H281DRAFT_00300 0.20 +0.0 320,539 - H281DRAFT_00300 0.20 -0.0 320,539 - H281DRAFT_00300 0.20 +0.0 320,539 - H281DRAFT_00300 0.20 +0.9 320,539 - H281DRAFT_00300 0.20 -0.2 320,539 - H281DRAFT_00300 0.20 +1.0 320,539 - H281DRAFT_00300 0.20 +0.3 320,539 - H281DRAFT_00300 0.20 +1.2 320,610 + H281DRAFT_00300 0.26 +0.0 320,611 - H281DRAFT_00300 0.26 +0.6 320,611 - H281DRAFT_00300 0.26 +0.2 320,643 + H281DRAFT_00300 0.28 +0.2 320,778 + H281DRAFT_00300 0.38 -0.4 320,778 + H281DRAFT_00300 0.38 -0.5 320,779 - H281DRAFT_00300 0.38 +0.1 320,805 + H281DRAFT_00300 0.40 +0.5 320,805 + H281DRAFT_00300 0.40 -0.8 320,805 + H281DRAFT_00300 0.40 +4.0 320,806 - H281DRAFT_00300 0.41 +0.9 320,992 - H281DRAFT_00300 0.55 -0.5 321,027 + H281DRAFT_00300 0.57 +0.6 321,027 + H281DRAFT_00300 0.57 +0.5 321,027 + H281DRAFT_00300 0.57 -0.8 321,027 + H281DRAFT_00300 0.57 -1.1 321,028 - H281DRAFT_00300 0.58 -0.1 321,028 - H281DRAFT_00300 0.58 +0.5 321,087 + H281DRAFT_00300 0.62 -0.6 321,087 + H281DRAFT_00300 0.62 -0.2 321,087 + H281DRAFT_00300 0.62 +0.7 321,088 - H281DRAFT_00300 0.62 +0.4 321,089 + H281DRAFT_00300 0.62 -0.7 321,090 - H281DRAFT_00300 0.62 -0.8 321,153 + H281DRAFT_00300 0.67 -1.0 321,195 + H281DRAFT_00300 0.70 +0.4 321,196 - H281DRAFT_00300 0.70 -0.7 321,307 - H281DRAFT_00300 0.79 -0.7 321,307 - H281DRAFT_00300 0.79 -0.2 321,307 - H281DRAFT_00300 0.79 +1.7 321,480 + -0.2 321,480 + -0.5 321,480 + -0.3 321,480 + -0.0 321,601 - -0.9 321,648 + +0.7 321,649 - +0.2 321,649 - +0.4 321,700 + +0.1 321,701 - +0.2 321,701 - +0.4 321,873 + -1.2 321,873 + -0.4 321,875 + -0.3 321,876 - +0.4 322,074 - +0.6
Or see this region's nucleotide sequence