Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3527 and Dsui_3528 are separated by 29 nucleotides Dsui_3528 and Dsui_3529 are separated by 17 nucleotides Dsui_3529 and Dsui_3530 are separated by 198 nucleotides Dsui_3530 and Dsui_3531 overlap by 4 nucleotides
Dsui_3527: Dsui_3527 - ketol-acid reductoisomerase, at 3,793,562 to 3,794,578
_3527
Dsui_3528: Dsui_3528 - acetolactate synthase, small subunit, at 3,794,608 to 3,795,099
_3528
Dsui_3529: Dsui_3529 - acetolactate synthase, large subunit, biosynthetic type, at 3,795,117 to 3,796,817
_3529
Dsui_3530: Dsui_3530 - DNA-directed RNA polymerase specialized sigma subunit, sigma24, at 3,797,016 to 3,797,573
_3530
Dsui_3531: Dsui_3531 - Protein of unknown function (DUF3619), at 3,797,570 to 3,797,941
_3531
Position (kb)
3795
3796
3797 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3794.245 kb on - strand, within Dsui_3527 at 3794.830 kb on - strand, within Dsui_3528 at 3795.109 kb on - strand at 3796.878 kb on + strand at 3796.893 kb on - strand at 3796.904 kb on - strand at 3796.907 kb on + strand at 3796.907 kb on + strand at 3796.931 kb on + strand at 3796.951 kb on - strand at 3796.951 kb on - strand at 3796.952 kb on - strand at 3797.002 kb on + strand at 3797.076 kb on - strand, within Dsui_3530 at 3797.078 kb on + strand, within Dsui_3530 at 3797.109 kb on + strand, within Dsui_3530 at 3797.117 kb on - strand, within Dsui_3530 at 3797.118 kb on - strand, within Dsui_3530 at 3797.138 kb on - strand, within Dsui_3530 at 3797.197 kb on - strand, within Dsui_3530 at 3797.314 kb on + strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand, within Dsui_3530 at 3797.322 kb on - strand at 3797.356 kb on + strand, within Dsui_3530 at 3797.356 kb on + strand, within Dsui_3530 at 3797.364 kb on - strand at 3797.374 kb on - strand, within Dsui_3530 at 3797.380 kb on - strand at 3797.421 kb on - strand at 3797.438 kb on - strand, within Dsui_3530 at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.527 kb on + strand at 3797.560 kb on + strand at 3797.567 kb on - strand at 3797.761 kb on + strand, within Dsui_3531 at 3797.775 kb on + strand, within Dsui_3531 at 3797.779 kb on + strand, within Dsui_3531 at 3797.787 kb on - strand, within Dsui_3531
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 3,794,245 - Dsui_3527 0.67 -6.7 3,794,830 - Dsui_3528 0.45 -1.9 3,795,109 - +0.6 3,796,878 + +1.0 3,796,893 - -3.0 3,796,904 - +0.4 3,796,907 + +0.6 3,796,907 + +1.1 3,796,931 + +1.1 3,796,951 - -1.0 3,796,951 - -0.8 3,796,952 - +0.3 3,797,002 + +1.1 3,797,076 - Dsui_3530 0.11 +0.5 3,797,078 + Dsui_3530 0.11 +0.9 3,797,109 + Dsui_3530 0.17 +0.7 3,797,117 - Dsui_3530 0.18 +1.9 3,797,118 - Dsui_3530 0.18 -3.8 3,797,138 - Dsui_3530 0.22 -3.8 3,797,197 - Dsui_3530 0.32 -2.6 3,797,314 + Dsui_3530 0.53 +1.3 3,797,322 - Dsui_3530 0.55 -0.2 3,797,322 - Dsui_3530 0.55 +1.2 3,797,322 - -0.7 3,797,356 + Dsui_3530 0.61 +0.6 3,797,356 + Dsui_3530 0.61 +0.9 3,797,364 - +2.1 3,797,374 - Dsui_3530 0.64 -0.0 3,797,380 - -2.7 3,797,421 - -1.3 3,797,438 - Dsui_3530 0.76 -4.0 3,797,527 + +0.7 3,797,527 + -2.4 3,797,527 + +1.3 3,797,527 + +0.8 3,797,560 + +0.7 3,797,567 - +1.3 3,797,761 + Dsui_3531 0.51 -2.6 3,797,775 + Dsui_3531 0.55 -3.8 3,797,779 + Dsui_3531 0.56 -0.2 3,797,787 - Dsui_3531 0.58 -0.7
Or see this region's nucleotide sequence