Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0659 and Dsui_0660 are separated by 21 nucleotides Dsui_0660 and Dsui_0661 are separated by 90 nucleotides Dsui_0661 and Dsui_0662 are separated by 91 nucleotides Dsui_0662 and Dsui_0663 overlap by 8 nucleotides Dsui_0663 and Dsui_0664 overlap by 14 nucleotides
Dsui_0659: Dsui_0659 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,093 to 658,455
_0659
Dsui_0660: Dsui_0660 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,477 to 658,875
_0660
Dsui_0661: Dsui_0661 - rubredoxin, at 658,966 to 659,166
_0661
Dsui_0662: Dsui_0662 - phosphomethylpyrimidine kinase, at 659,258 to 660,100
_0662
Dsui_0663: Dsui_0663 - thiamine-phosphate pyrophosphorylase, at 660,093 to 660,740
_0663
Dsui_0664: Dsui_0664 - glutamate-1-semialdehyde-2,1-aminomutase, at 660,727 to 662,010
_0664
Position (kb) 659
660
661 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 658.316 kb on - strand, within Dsui_0659 at 658.328 kb on + strand at 658.457 kb on + strand at 658.458 kb on - strand at 658.459 kb on - strand at 658.486 kb on - strand at 658.496 kb on - strand at 658.554 kb on + strand, within Dsui_0660 at 658.562 kb on - strand, within Dsui_0660 at 658.582 kb on - strand, within Dsui_0660 at 658.619 kb on + strand at 658.738 kb on - strand, within Dsui_0660 at 658.772 kb on - strand, within Dsui_0660 at 658.850 kb on - strand at 658.860 kb on - strand at 658.896 kb on + strand at 658.904 kb on - strand at 658.910 kb on - strand at 658.914 kb on - strand at 659.012 kb on + strand, within Dsui_0661 at 659.150 kb on + strand at 659.156 kb on + strand at 659.174 kb on - strand at 659.174 kb on - strand at 659.242 kb on - strand at 659.297 kb on + strand at 660.104 kb on + strand at 660.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 658,316 - Dsui_0659 0.61 +2.8 658,328 + -0.1 658,457 + -0.8 658,458 - +1.4 658,459 - +0.6 658,486 - +1.5 658,496 - +2.7 658,554 + Dsui_0660 0.19 +0.3 658,562 - Dsui_0660 0.21 +2.9 658,582 - Dsui_0660 0.26 +3.5 658,619 + +4.4 658,738 - Dsui_0660 0.65 +2.7 658,772 - Dsui_0660 0.74 +2.0 658,850 - +3.5 658,860 - +1.4 658,896 + -0.3 658,904 - +2.0 658,910 - +0.7 658,914 - +2.0 659,012 + Dsui_0661 0.23 -1.1 659,150 + -4.1 659,156 + -1.9 659,174 - +2.5 659,174 - +1.8 659,242 - +0.6 659,297 + +0.3 660,104 + +0.9 660,723 + +0.1
Or see this region's nucleotide sequence