Experiment: with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0543 and Dsui_0544 are separated by 124 nucleotides Dsui_0544 and Dsui_0545 are separated by 94 nucleotides Dsui_0545 and Dsui_0546 are separated by 81 nucleotides Dsui_0546 and Dsui_0547 are separated by 60 nucleotides Dsui_0547 and Dsui_0548 are separated by 72 nucleotides
Dsui_0543: Dsui_0543 - malic enzyme, at 547,934 to 550,210
_0543
Dsui_0544: Dsui_0544 - flagellar basal body-associated protein, at 550,335 to 550,724
_0544
Dsui_0545: Dsui_0545 - tRNA-Thr, at 550,819 to 550,893
_0545
Dsui_0546: Dsui_0546 - tRNA-Lys, at 550,975 to 551,050
_0546
Dsui_0547: Dsui_0547 - tRNA-Lys, at 551,111 to 551,186
_0547
Dsui_0548: Dsui_0548 - putative transporter component, at 551,259 to 552,365
_0548
Position (kb) 550
551 Strain fitness (log2 ratio) -2
-1
0
1
2 at 549.355 kb on - strand, within Dsui_0543 at 549.393 kb on + strand, within Dsui_0543 at 549.552 kb on + strand, within Dsui_0543 at 549.810 kb on + strand, within Dsui_0543 at 549.810 kb on + strand, within Dsui_0543 at 549.818 kb on - strand, within Dsui_0543 at 549.901 kb on + strand, within Dsui_0543 at 549.963 kb on - strand, within Dsui_0543 at 550.219 kb on + strand at 550.227 kb on - strand at 550.232 kb on - strand at 550.301 kb on + strand at 550.378 kb on + strand, within Dsui_0544 at 550.385 kb on - strand, within Dsui_0544 at 550.508 kb on + strand, within Dsui_0544 at 550.689 kb on + strand at 550.699 kb on + strand at 550.707 kb on - strand at 550.738 kb on - strand at 550.751 kb on - strand at 550.754 kb on + strand at 550.799 kb on - strand at 550.806 kb on - strand at 550.809 kb on + strand at 551.340 kb on + strand at 551.391 kb on - strand, within Dsui_0548 at 551.529 kb on - strand, within Dsui_0548 at 551.585 kb on + strand at 551.616 kb on + strand, within Dsui_0548
Per-strain Table
Position Strand Gene LocusTag Fraction with 1 mM Stressor (H2S) + 10 mM ClO4 and 20 mM lactate remove 549,355 - Dsui_0543 0.62 +1.4 549,393 + Dsui_0543 0.64 +0.1 549,552 + Dsui_0543 0.71 +2.2 549,810 + Dsui_0543 0.82 +0.7 549,810 + Dsui_0543 0.82 +0.5 549,818 - Dsui_0543 0.83 +0.9 549,901 + Dsui_0543 0.86 -0.3 549,963 - Dsui_0543 0.89 -1.1 550,219 + -2.6 550,227 - +0.7 550,232 - +1.2 550,301 + -0.9 550,378 + Dsui_0544 0.11 +1.1 550,385 - Dsui_0544 0.13 +0.4 550,508 + Dsui_0544 0.44 +1.0 550,689 + +0.1 550,699 + -0.2 550,707 - +1.0 550,738 - +1.0 550,751 - -1.6 550,754 + +0.9 550,799 - -1.8 550,806 - +1.0 550,809 + +0.6 551,340 + +1.2 551,391 - Dsui_0548 0.12 +1.1 551,529 - Dsui_0548 0.24 +1.6 551,585 + +1.3 551,616 + Dsui_0548 0.32 +0.9
Or see this region's nucleotide sequence