Experiment: ICP3_phage_41.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_12535 and CSW01_12545 are separated by 199 nucleotides CSW01_12545 and CSW01_12550 are separated by 109 nucleotides CSW01_12550 and CSW01_12555 are separated by 253 nucleotides
CSW01_12535: CSW01_12535 - cell division protein ZapA, at 2,689,308 to 2,689,616
_12535
CSW01_12545: CSW01_12545 - 5-formyltetrahydrofolate cyclo-ligase, at 2,689,816 to 2,690,400
_12545
CSW01_12550: CSW01_12550 - ribose-5-phosphate isomerase, at 2,690,510 to 2,691,166
_12550
CSW01_12555: CSW01_12555 - phosphoglycerate dehydrogenase, at 2,691,420 to 2,692,649
_12555
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2689.560 kb on - strand, within CSW01_12535 at 2689.610 kb on + strand at 2689.630 kb on - strand at 2689.632 kb on + strand at 2689.635 kb on - strand at 2689.701 kb on - strand at 2689.701 kb on - strand at 2689.773 kb on + strand at 2689.781 kb on - strand at 2689.834 kb on + strand at 2689.842 kb on - strand at 2689.911 kb on - strand, within CSW01_12545 at 2689.950 kb on + strand, within CSW01_12545 at 2689.960 kb on - strand, within CSW01_12545 at 2689.960 kb on - strand, within CSW01_12545 at 2689.960 kb on - strand, within CSW01_12545 at 2689.965 kb on - strand, within CSW01_12545 at 2689.983 kb on + strand, within CSW01_12545 at 2689.983 kb on + strand, within CSW01_12545 at 2689.991 kb on - strand, within CSW01_12545 at 2689.991 kb on - strand, within CSW01_12545 at 2689.991 kb on - strand, within CSW01_12545 at 2690.024 kb on + strand, within CSW01_12545 at 2690.040 kb on - strand, within CSW01_12545 at 2690.040 kb on - strand, within CSW01_12545 at 2690.052 kb on - strand, within CSW01_12545 at 2690.087 kb on + strand, within CSW01_12545 at 2690.087 kb on + strand, within CSW01_12545 at 2690.095 kb on - strand, within CSW01_12545 at 2690.133 kb on + strand, within CSW01_12545 at 2690.133 kb on + strand, within CSW01_12545 at 2690.133 kb on + strand, within CSW01_12545 at 2690.133 kb on + strand, within CSW01_12545 at 2690.133 kb on + strand, within CSW01_12545 at 2690.141 kb on - strand, within CSW01_12545 at 2690.141 kb on - strand, within CSW01_12545 at 2690.157 kb on - strand, within CSW01_12545 at 2690.157 kb on - strand, within CSW01_12545 at 2690.157 kb on - strand, within CSW01_12545 at 2690.157 kb on - strand, within CSW01_12545 at 2690.220 kb on + strand, within CSW01_12545 at 2690.263 kb on - strand, within CSW01_12545 at 2690.337 kb on - strand, within CSW01_12545 at 2690.385 kb on + strand at 2690.414 kb on - strand at 2690.424 kb on - strand at 2690.424 kb on - strand at 2690.424 kb on - strand at 2690.458 kb on - strand at 2690.462 kb on + strand at 2691.381 kb on + strand at 2691.448 kb on + strand at 2691.448 kb on + strand at 2691.456 kb on - strand at 2691.474 kb on + strand at 2691.482 kb on - strand at 2691.487 kb on - strand at 2691.538 kb on - strand at 2691.550 kb on + strand, within CSW01_12555 at 2691.560 kb on + strand, within CSW01_12555 at 2691.560 kb on + strand, within CSW01_12555 at 2691.560 kb on + strand, within CSW01_12555 at 2691.673 kb on + strand, within CSW01_12555 at 2691.673 kb on + strand, within CSW01_12555 at 2691.673 kb on + strand, within CSW01_12555 at 2691.681 kb on - strand, within CSW01_12555 at 2691.710 kb on + strand, within CSW01_12555 at 2691.720 kb on - strand, within CSW01_12555 at 2691.722 kb on - strand, within CSW01_12555 at 2691.824 kb on - strand, within CSW01_12555 at 2691.914 kb on + strand, within CSW01_12555 at 2691.991 kb on - strand, within CSW01_12555 at 2691.999 kb on + strand, within CSW01_12555 at 2692.006 kb on + strand, within CSW01_12555 at 2692.007 kb on - strand, within CSW01_12555 at 2692.020 kb on + strand, within CSW01_12555 at 2692.030 kb on - strand, within CSW01_12555 at 2692.036 kb on - strand, within CSW01_12555 at 2692.092 kb on - strand, within CSW01_12555 at 2692.111 kb on - strand, within CSW01_12555
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep2 remove 2,689,560 - CSW01_12535 0.82 -1.8 2,689,610 + -1.5 2,689,630 - +1.1 2,689,632 + +0.8 2,689,635 - +0.3 2,689,701 - +0.1 2,689,701 - -0.1 2,689,773 + -3.7 2,689,781 - +0.8 2,689,834 + +1.2 2,689,842 - -1.1 2,689,911 - CSW01_12545 0.16 +0.1 2,689,950 + CSW01_12545 0.23 +0.6 2,689,960 - CSW01_12545 0.25 -3.1 2,689,960 - CSW01_12545 0.25 -1.5 2,689,960 - CSW01_12545 0.25 -0.9 2,689,965 - CSW01_12545 0.25 -2.0 2,689,983 + CSW01_12545 0.29 -1.0 2,689,983 + CSW01_12545 0.29 +0.8 2,689,991 - CSW01_12545 0.30 -2.3 2,689,991 - CSW01_12545 0.30 +0.5 2,689,991 - CSW01_12545 0.30 +0.2 2,690,024 + CSW01_12545 0.36 +0.9 2,690,040 - CSW01_12545 0.38 +0.2 2,690,040 - CSW01_12545 0.38 -0.3 2,690,052 - CSW01_12545 0.40 -2.1 2,690,087 + CSW01_12545 0.46 -0.1 2,690,087 + CSW01_12545 0.46 -1.8 2,690,095 - CSW01_12545 0.48 +1.2 2,690,133 + CSW01_12545 0.54 +1.0 2,690,133 + CSW01_12545 0.54 +0.9 2,690,133 + CSW01_12545 0.54 -1.2 2,690,133 + CSW01_12545 0.54 -0.5 2,690,133 + CSW01_12545 0.54 -0.6 2,690,141 - CSW01_12545 0.56 -1.5 2,690,141 - CSW01_12545 0.56 +0.9 2,690,157 - CSW01_12545 0.58 -2.6 2,690,157 - CSW01_12545 0.58 +1.0 2,690,157 - CSW01_12545 0.58 +0.4 2,690,157 - CSW01_12545 0.58 +0.1 2,690,220 + CSW01_12545 0.69 -1.9 2,690,263 - CSW01_12545 0.76 +1.5 2,690,337 - CSW01_12545 0.89 +1.1 2,690,385 + -2.7 2,690,414 - +1.5 2,690,424 - -1.1 2,690,424 - -2.7 2,690,424 - +1.8 2,690,458 - -0.3 2,690,462 + +1.6 2,691,381 + +1.7 2,691,448 + -0.4 2,691,448 + -0.0 2,691,456 - -1.3 2,691,474 + -2.1 2,691,482 - -0.7 2,691,487 - +1.0 2,691,538 - -2.2 2,691,550 + CSW01_12555 0.11 +1.0 2,691,560 + CSW01_12555 0.11 -3.0 2,691,560 + CSW01_12555 0.11 +1.1 2,691,560 + CSW01_12555 0.11 +0.4 2,691,673 + CSW01_12555 0.21 -3.2 2,691,673 + CSW01_12555 0.21 -1.4 2,691,673 + CSW01_12555 0.21 -0.7 2,691,681 - CSW01_12555 0.21 +1.0 2,691,710 + CSW01_12555 0.24 +0.7 2,691,720 - CSW01_12555 0.24 +1.6 2,691,722 - CSW01_12555 0.25 -4.2 2,691,824 - CSW01_12555 0.33 +0.9 2,691,914 + CSW01_12555 0.40 -0.2 2,691,991 - CSW01_12555 0.46 +1.3 2,691,999 + CSW01_12555 0.47 -2.8 2,692,006 + CSW01_12555 0.48 +0.4 2,692,007 - CSW01_12555 0.48 -2.9 2,692,020 + CSW01_12555 0.49 -2.1 2,692,030 - CSW01_12555 0.50 +1.5 2,692,036 - CSW01_12555 0.50 +0.4 2,692,092 - CSW01_12555 0.55 +1.5 2,692,111 - CSW01_12555 0.56 +0.2
Or see this region's nucleotide sequence