Experiment: ICP3_phage_41.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_11955 and CSW01_11960 are separated by 345 nucleotides CSW01_11960 and CSW01_11965 are separated by 79 nucleotides
CSW01_11955: CSW01_11955 - endonuclease, at 2,547,332 to 2,548,192
_11955
CSW01_11960: CSW01_11960 - autonomous glycyl radical cofactor GrcA, at 2,548,538 to 2,548,915
_11960
CSW01_11965: CSW01_11965 - threonine synthase, at 2,548,995 to 2,550,275
_11965
Position (kb)
2548
2549 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2547.573 kb on + strand, within CSW01_11955 at 2547.581 kb on - strand, within CSW01_11955 at 2547.628 kb on - strand, within CSW01_11955 at 2547.742 kb on - strand, within CSW01_11955 at 2547.742 kb on - strand, within CSW01_11955 at 2547.742 kb on - strand, within CSW01_11955 at 2547.854 kb on + strand, within CSW01_11955 at 2547.882 kb on - strand, within CSW01_11955 at 2547.897 kb on - strand, within CSW01_11955 at 2547.970 kb on + strand, within CSW01_11955 at 2548.044 kb on - strand, within CSW01_11955 at 2548.068 kb on - strand, within CSW01_11955 at 2548.096 kb on + strand, within CSW01_11955 at 2548.157 kb on + strand at 2548.200 kb on - strand at 2548.543 kb on - strand at 2548.582 kb on + strand, within CSW01_11960 at 2548.590 kb on + strand, within CSW01_11960 at 2548.629 kb on + strand, within CSW01_11960 at 2548.658 kb on + strand, within CSW01_11960 at 2548.706 kb on - strand, within CSW01_11960 at 2548.776 kb on + strand, within CSW01_11960 at 2548.865 kb on + strand, within CSW01_11960 at 2549.038 kb on + strand at 2549.053 kb on - strand at 2549.058 kb on - strand at 2549.176 kb on - strand, within CSW01_11965 at 2549.244 kb on - strand, within CSW01_11965 at 2549.296 kb on + strand, within CSW01_11965 at 2549.439 kb on + strand, within CSW01_11965 at 2549.493 kb on + strand, within CSW01_11965 at 2549.493 kb on + strand, within CSW01_11965 at 2549.501 kb on - strand, within CSW01_11965 at 2549.501 kb on - strand, within CSW01_11965 at 2549.501 kb on - strand, within CSW01_11965 at 2549.501 kb on - strand, within CSW01_11965 at 2549.501 kb on - strand, within CSW01_11965 at 2549.521 kb on - strand, within CSW01_11965 at 2549.583 kb on - strand, within CSW01_11965 at 2549.655 kb on + strand, within CSW01_11965 at 2549.655 kb on + strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.663 kb on - strand, within CSW01_11965 at 2549.673 kb on + strand, within CSW01_11965 at 2549.678 kb on + strand, within CSW01_11965 at 2549.681 kb on - strand, within CSW01_11965
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep2 remove 2,547,573 + CSW01_11955 0.28 -2.0 2,547,581 - CSW01_11955 0.29 +0.5 2,547,628 - CSW01_11955 0.34 +0.2 2,547,742 - CSW01_11955 0.48 -0.2 2,547,742 - CSW01_11955 0.48 +0.5 2,547,742 - CSW01_11955 0.48 +0.6 2,547,854 + CSW01_11955 0.61 +0.3 2,547,882 - CSW01_11955 0.64 +0.7 2,547,897 - CSW01_11955 0.66 +0.3 2,547,970 + CSW01_11955 0.74 +1.2 2,548,044 - CSW01_11955 0.83 -0.4 2,548,068 - CSW01_11955 0.85 +1.1 2,548,096 + CSW01_11955 0.89 -0.3 2,548,157 + +1.6 2,548,200 - -0.5 2,548,543 - +0.2 2,548,582 + CSW01_11960 0.12 +0.6 2,548,590 + CSW01_11960 0.14 -0.7 2,548,629 + CSW01_11960 0.24 -4.1 2,548,658 + CSW01_11960 0.32 +1.0 2,548,706 - CSW01_11960 0.44 -4.0 2,548,776 + CSW01_11960 0.63 +1.0 2,548,865 + CSW01_11960 0.87 -1.1 2,549,038 + +0.5 2,549,053 - -0.2 2,549,058 - +0.6 2,549,176 - CSW01_11965 0.14 -3.2 2,549,244 - CSW01_11965 0.19 -3.7 2,549,296 + CSW01_11965 0.23 -0.0 2,549,439 + CSW01_11965 0.35 +0.8 2,549,493 + CSW01_11965 0.39 -2.2 2,549,493 + CSW01_11965 0.39 -2.7 2,549,501 - CSW01_11965 0.40 +0.4 2,549,501 - CSW01_11965 0.40 +2.0 2,549,501 - CSW01_11965 0.40 -2.6 2,549,501 - CSW01_11965 0.40 +0.9 2,549,501 - CSW01_11965 0.40 -1.0 2,549,521 - CSW01_11965 0.41 -2.9 2,549,583 - CSW01_11965 0.46 -0.0 2,549,655 + CSW01_11965 0.52 +0.4 2,549,655 + CSW01_11965 0.52 -0.0 2,549,663 - CSW01_11965 0.52 -0.2 2,549,663 - CSW01_11965 0.52 +1.0 2,549,663 - CSW01_11965 0.52 +0.4 2,549,663 - CSW01_11965 0.52 +1.2 2,549,663 - CSW01_11965 0.52 -1.3 2,549,663 - CSW01_11965 0.52 +0.9 2,549,663 - CSW01_11965 0.52 +2.1 2,549,663 - CSW01_11965 0.52 +0.2 2,549,663 - CSW01_11965 0.52 +1.7 2,549,663 - CSW01_11965 0.52 +0.7 2,549,673 + CSW01_11965 0.53 +0.5 2,549,678 + CSW01_11965 0.53 -0.5 2,549,681 - CSW01_11965 0.54 -0.2
Or see this region's nucleotide sequence