Strain Fitness in Vibrio cholerae E7946 ATCC 55056 around CSW01_10280

Experiment: ICP3_phage_41.1_MOI_rep2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCSW01_10265 and CSW01_10270 overlap by 4 nucleotidesCSW01_10270 and CSW01_10275 overlap by 4 nucleotidesCSW01_10275 and CSW01_10280 overlap by 1 nucleotidesCSW01_10280 and CSW01_10285 are separated by 90 nucleotidesCSW01_10285 and CSW01_10290 overlap by 4 nucleotides CSW01_10265: CSW01_10265 - c-type cytochrome biogenesis protein CcmF, at 2,207,282 to 2,209,237 _10265 CSW01_10270: CSW01_10270 - cytochrome c-type biogenesis protein CcmE, at 2,209,234 to 2,209,716 _10270 CSW01_10275: CSW01_10275 - heme exporter protein CcmD, at 2,209,713 to 2,209,919 _10275 CSW01_10280: CSW01_10280 - heme exporter protein C, at 2,209,919 to 2,210,671 _10280 CSW01_10285: CSW01_10285 - heme exporter protein CcmB, at 2,210,762 to 2,211,430 _10285 CSW01_10290: CSW01_10290 - cytochrome c biogenesis ATP-binding export protein CcmA, at 2,211,427 to 2,212,050 _10290 Position (kb) 2209 2210 2211Strain fitness (log2 ratio) -3 -2 -1 0 1at 2208.973 kb on + strand, within CSW01_10265at 2208.986 kb on - strand, within CSW01_10265at 2209.138 kb on - strandat 2209.275 kb on - strandat 2209.332 kb on - strand, within CSW01_10270at 2209.399 kb on + strand, within CSW01_10270at 2209.399 kb on + strand, within CSW01_10270at 2209.402 kb on - strand, within CSW01_10270at 2209.442 kb on - strand, within CSW01_10270at 2209.454 kb on - strand, within CSW01_10270at 2209.607 kb on - strand, within CSW01_10270at 2209.644 kb on + strand, within CSW01_10270at 2209.652 kb on - strand, within CSW01_10270at 2209.960 kb on + strandat 2209.968 kb on - strandat 2210.135 kb on - strand, within CSW01_10280at 2210.137 kb on + strand, within CSW01_10280at 2210.150 kb on - strand, within CSW01_10280at 2210.211 kb on + strand, within CSW01_10280at 2210.248 kb on + strand, within CSW01_10280at 2210.275 kb on + strand, within CSW01_10280at 2210.275 kb on + strand, within CSW01_10280at 2210.283 kb on - strand, within CSW01_10280at 2210.290 kb on - strand, within CSW01_10280at 2210.360 kb on + strand, within CSW01_10280at 2210.360 kb on + strand, within CSW01_10280at 2210.360 kb on + strand, within CSW01_10280at 2210.360 kb on + strand, within CSW01_10280at 2210.365 kb on + strand, within CSW01_10280at 2210.368 kb on - strand, within CSW01_10280at 2210.368 kb on - strand, within CSW01_10280at 2210.491 kb on + strand, within CSW01_10280at 2210.648 kb on - strandat 2210.661 kb on - strandat 2210.828 kb on - strandat 2210.828 kb on - strandat 2210.911 kb on - strand, within CSW01_10285at 2211.015 kb on - strand, within CSW01_10285at 2211.090 kb on - strand, within CSW01_10285at 2211.114 kb on + strand, within CSW01_10285at 2211.129 kb on + strand, within CSW01_10285at 2211.304 kb on + strand, within CSW01_10285at 2211.354 kb on + strand, within CSW01_10285at 2211.354 kb on + strand, within CSW01_10285at 2211.459 kb on + strandat 2211.459 kb on + strandat 2211.459 kb on + strandat 2211.459 kb on + strandat 2211.459 kb on + strandat 2211.459 kb on + strandat 2211.467 kb on - strandat 2211.467 kb on - strandat 2211.467 kb on - strandat 2211.506 kb on + strand, within CSW01_10290at 2211.540 kb on + strand, within CSW01_10290at 2211.557 kb on - strand, within CSW01_10290at 2211.643 kb on + strand, within CSW01_10290at 2211.653 kb on + strand, within CSW01_10290at 2211.653 kb on + strand, within CSW01_10290at 2211.661 kb on - strand, within CSW01_10290at 2211.661 kb on - strand, within CSW01_10290

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Per-strain Table

Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep2
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2,208,973 + CSW01_10265 0.86 -2.4
2,208,986 - CSW01_10265 0.87 -1.5
2,209,138 - -2.2
2,209,275 - -0.3
2,209,332 - CSW01_10270 0.20 +0.4
2,209,399 + CSW01_10270 0.34 -1.5
2,209,399 + CSW01_10270 0.34 -1.3
2,209,402 - CSW01_10270 0.35 -1.5
2,209,442 - CSW01_10270 0.43 -0.6
2,209,454 - CSW01_10270 0.46 -2.9
2,209,607 - CSW01_10270 0.77 -2.9
2,209,644 + CSW01_10270 0.85 -1.1
2,209,652 - CSW01_10270 0.87 -1.0
2,209,960 + -3.2
2,209,968 - -1.9
2,210,135 - CSW01_10280 0.29 -1.5
2,210,137 + CSW01_10280 0.29 -2.5
2,210,150 - CSW01_10280 0.31 -1.6
2,210,211 + CSW01_10280 0.39 -1.1
2,210,248 + CSW01_10280 0.44 -0.6
2,210,275 + CSW01_10280 0.47 -1.0
2,210,275 + CSW01_10280 0.47 -0.7
2,210,283 - CSW01_10280 0.48 -3.2
2,210,290 - CSW01_10280 0.49 -1.5
2,210,360 + CSW01_10280 0.59 -0.6
2,210,360 + CSW01_10280 0.59 -3.0
2,210,360 + CSW01_10280 0.59 -2.5
2,210,360 + CSW01_10280 0.59 -2.2
2,210,365 + CSW01_10280 0.59 -2.1
2,210,368 - CSW01_10280 0.60 +0.4
2,210,368 - CSW01_10280 0.60 -2.2
2,210,491 + CSW01_10280 0.76 -1.6
2,210,648 - -3.1
2,210,661 - -0.8
2,210,828 - -0.6
2,210,828 - +0.0
2,210,911 - CSW01_10285 0.22 -1.2
2,211,015 - CSW01_10285 0.38 +0.1
2,211,090 - CSW01_10285 0.49 -0.3
2,211,114 + CSW01_10285 0.53 -0.7
2,211,129 + CSW01_10285 0.55 -0.9
2,211,304 + CSW01_10285 0.81 +0.3
2,211,354 + CSW01_10285 0.88 -2.7
2,211,354 + CSW01_10285 0.88 -2.0
2,211,459 + -2.1
2,211,459 + -2.1
2,211,459 + -1.0
2,211,459 + -1.6
2,211,459 + -2.9
2,211,459 + -1.6
2,211,467 - -1.0
2,211,467 - -2.9
2,211,467 - -1.4
2,211,506 + CSW01_10290 0.13 +0.1
2,211,540 + CSW01_10290 0.18 -0.6
2,211,557 - CSW01_10290 0.21 +0.4
2,211,643 + CSW01_10290 0.35 -0.5
2,211,653 + CSW01_10290 0.36 -1.4
2,211,653 + CSW01_10290 0.36 -3.5
2,211,661 - CSW01_10290 0.38 -1.2
2,211,661 - CSW01_10290 0.38 -0.4

Or see this region's nucleotide sequence