Experiment: ICP3_phage_41.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03485 and CSW01_03490 are separated by 169 nucleotides CSW01_03490 and CSW01_03495 are separated by 30 nucleotides
CSW01_03485: CSW01_03485 - branched-chain amino acid transport system II carrier protein, at 706,721 to 708,034
_03485
CSW01_03490: CSW01_03490 - peptide chain release factor 2, at 708,204 to 709,302
_03490
CSW01_03495: CSW01_03495 - lysine--tRNA ligase, at 709,333 to 710,865
_03495
Position (kb) 708
709
710 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 707.208 kb on - strand, within CSW01_03485 at 707.210 kb on + strand, within CSW01_03485 at 707.240 kb on - strand, within CSW01_03485 at 707.284 kb on - strand, within CSW01_03485 at 707.308 kb on - strand, within CSW01_03485 at 707.310 kb on - strand, within CSW01_03485 at 707.372 kb on + strand, within CSW01_03485 at 707.381 kb on - strand, within CSW01_03485 at 707.412 kb on + strand, within CSW01_03485 at 707.420 kb on - strand, within CSW01_03485 at 707.420 kb on - strand, within CSW01_03485 at 707.446 kb on + strand, within CSW01_03485 at 707.454 kb on - strand, within CSW01_03485 at 707.461 kb on + strand, within CSW01_03485 at 707.469 kb on - strand, within CSW01_03485 at 707.481 kb on + strand, within CSW01_03485 at 707.481 kb on + strand, within CSW01_03485 at 707.487 kb on - strand, within CSW01_03485 at 707.489 kb on - strand, within CSW01_03485 at 707.561 kb on - strand, within CSW01_03485 at 707.636 kb on - strand, within CSW01_03485 at 707.680 kb on + strand, within CSW01_03485 at 707.688 kb on - strand, within CSW01_03485 at 707.708 kb on - strand, within CSW01_03485 at 707.728 kb on + strand, within CSW01_03485 at 707.728 kb on + strand, within CSW01_03485 at 707.728 kb on + strand, within CSW01_03485 at 707.736 kb on - strand, within CSW01_03485 at 707.759 kb on + strand, within CSW01_03485 at 707.759 kb on + strand, within CSW01_03485 at 707.767 kb on - strand, within CSW01_03485 at 707.846 kb on - strand, within CSW01_03485 at 707.846 kb on - strand, within CSW01_03485 at 707.875 kb on - strand, within CSW01_03485 at 707.903 kb on - strand at 707.923 kb on + strand at 707.939 kb on + strand at 707.974 kb on + strand at 707.977 kb on - strand at 707.977 kb on - strand at 708.037 kb on - strand at 708.047 kb on - strand at 708.058 kb on - strand at 708.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep2 remove 707,208 - CSW01_03485 0.37 -0.8 707,210 + CSW01_03485 0.37 -1.8 707,240 - CSW01_03485 0.39 +1.9 707,284 - CSW01_03485 0.43 -0.5 707,308 - CSW01_03485 0.45 -0.3 707,310 - CSW01_03485 0.45 -1.2 707,372 + CSW01_03485 0.50 -1.5 707,381 - CSW01_03485 0.50 -0.1 707,412 + CSW01_03485 0.53 -1.5 707,420 - CSW01_03485 0.53 -2.7 707,420 - CSW01_03485 0.53 +0.2 707,446 + CSW01_03485 0.55 -3.0 707,454 - CSW01_03485 0.56 -0.6 707,461 + CSW01_03485 0.56 +1.2 707,469 - CSW01_03485 0.57 +0.1 707,481 + CSW01_03485 0.58 -2.5 707,481 + CSW01_03485 0.58 -1.2 707,487 - CSW01_03485 0.58 +0.3 707,489 - CSW01_03485 0.58 +0.2 707,561 - CSW01_03485 0.64 -1.6 707,636 - CSW01_03485 0.70 +1.1 707,680 + CSW01_03485 0.73 -3.0 707,688 - CSW01_03485 0.74 +0.5 707,708 - CSW01_03485 0.75 -1.4 707,728 + CSW01_03485 0.77 -2.1 707,728 + CSW01_03485 0.77 -2.2 707,728 + CSW01_03485 0.77 -2.0 707,736 - CSW01_03485 0.77 -2.4 707,759 + CSW01_03485 0.79 +1.0 707,759 + CSW01_03485 0.79 +1.1 707,767 - CSW01_03485 0.80 -3.6 707,846 - CSW01_03485 0.86 -1.0 707,846 - CSW01_03485 0.86 -2.0 707,875 - CSW01_03485 0.88 +0.3 707,903 - -0.4 707,923 + -2.5 707,939 + +2.4 707,974 + -0.9 707,977 - +0.3 707,977 - +0.9 708,037 - -3.6 708,047 - +0.2 708,058 - +1.2 708,058 - -2.9
Or see this region's nucleotide sequence