Experiment: ICP3_phage_41.1_MOI_rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_03055 and CSW01_03060 are separated by 99 nucleotides CSW01_03060 and CSW01_03065 overlap by 1 nucleotides CSW01_03065 and CSW01_03070 are separated by 67 nucleotides CSW01_03070 and CSW01_03075 are separated by 2 nucleotides
CSW01_03055: CSW01_03055 - cytochrome c1, at 604,815 to 605,552
_03055
CSW01_03060: CSW01_03060 - stringent starvation protein A, at 605,652 to 606,287
_03060
CSW01_03065: CSW01_03065 - ClpXP protease specificity-enhancing factor, at 606,287 to 606,763
_03065
CSW01_03070: CSW01_03070 - hemolysin, at 606,831 to 607,400
_03070
CSW01_03075: CSW01_03075 - phosphoheptose isomerase, at 607,403 to 607,993
_03075
Position (kb) 606
607 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 605.287 kb on - strand, within CSW01_03055 at 605.358 kb on - strand, within CSW01_03055 at 605.403 kb on + strand, within CSW01_03055 at 605.566 kb on - strand at 605.602 kb on + strand at 605.602 kb on + strand at 605.645 kb on - strand at 606.483 kb on + strand, within CSW01_03065 at 606.599 kb on + strand, within CSW01_03065 at 606.709 kb on - strand, within CSW01_03065 at 606.727 kb on + strand at 606.905 kb on - strand, within CSW01_03070 at 606.976 kb on + strand, within CSW01_03070 at 607.099 kb on + strand, within CSW01_03070 at 607.200 kb on - strand, within CSW01_03070 at 607.327 kb on + strand, within CSW01_03070 at 607.337 kb on + strand, within CSW01_03070 at 607.355 kb on - strand at 607.355 kb on - strand at 607.357 kb on - strand at 607.385 kb on + strand at 607.393 kb on - strand at 607.393 kb on - strand at 607.393 kb on - strand at 607.454 kb on + strand at 607.459 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ICP3_phage_41.1_MOI_rep2 remove 605,287 - CSW01_03055 0.64 +0.2 605,358 - CSW01_03055 0.74 -1.9 605,403 + CSW01_03055 0.80 +0.7 605,566 - -0.6 605,602 + -3.1 605,602 + -0.5 605,645 - -0.9 606,483 + CSW01_03065 0.41 -4.8 606,599 + CSW01_03065 0.65 -0.2 606,709 - CSW01_03065 0.88 -1.9 606,727 + +1.1 606,905 - CSW01_03070 0.13 -1.9 606,976 + CSW01_03070 0.25 +2.0 607,099 + CSW01_03070 0.47 -1.1 607,200 - CSW01_03070 0.65 -2.4 607,327 + CSW01_03070 0.87 +0.6 607,337 + CSW01_03070 0.89 +1.1 607,355 - -0.7 607,355 - +1.3 607,357 - -2.3 607,385 + +1.0 607,393 - +2.0 607,393 - -0.4 607,393 - -0.2 607,454 + -2.1 607,459 - -3.7
Or see this region's nucleotide sequence