Experiment: Choline chloride 450 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2595 and Shewana3_2596 are separated by 138 nucleotides Shewana3_2596 and Shewana3_2597 are separated by 131 nucleotides Shewana3_2597 and Shewana3_2598 are separated by 311 nucleotides Shewana3_2598 and Shewana3_2599 are separated by 64 nucleotides
Shewana3_2595: Shewana3_2595 - carbon starvation protein CstA (RefSeq), at 3,099,057 to 3,100,511
_2595
Shewana3_2596: Shewana3_2596 - hypothetical protein (RefSeq), at 3,100,650 to 3,101,018
_2596
Shewana3_2597: Shewana3_2597 - L-sorbosone dehydrogenase (SNDH) (RefSeq), at 3,101,150 to 3,102,292
_2597
Shewana3_2598: Shewana3_2598 - hypothetical protein (RefSeq), at 3,102,604 to 3,103,050
_2598
Shewana3_2599: Shewana3_2599 - hypothetical protein (RefSeq), at 3,103,115 to 3,103,984
_2599
Position (kb)
3101
3102
3103 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3100.253 kb on + strand, within Shewana3_2595 at 3100.385 kb on - strand at 3100.434 kb on + strand at 3100.629 kb on + strand at 3100.637 kb on - strand at 3101.491 kb on - strand, within Shewana3_2597 at 3101.515 kb on - strand, within Shewana3_2597 at 3101.968 kb on + strand, within Shewana3_2597 at 3102.036 kb on - strand, within Shewana3_2597 at 3102.088 kb on - strand, within Shewana3_2597 at 3102.266 kb on - strand at 3102.565 kb on - strand at 3102.888 kb on + strand, within Shewana3_2598 at 3103.045 kb on + strand at 3103.053 kb on - strand at 3103.239 kb on - strand, within Shewana3_2599
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 450 mM remove 3,100,253 + Shewana3_2595 0.82 -0.0 3,100,385 - -1.3 3,100,434 + -1.0 3,100,629 + -1.6 3,100,637 - +0.1 3,101,491 - Shewana3_2597 0.30 -2.8 3,101,515 - Shewana3_2597 0.32 +2.0 3,101,968 + Shewana3_2597 0.72 +0.7 3,102,036 - Shewana3_2597 0.78 +0.7 3,102,088 - Shewana3_2597 0.82 -0.6 3,102,266 - -0.3 3,102,565 - +0.7 3,102,888 + Shewana3_2598 0.64 +0.7 3,103,045 + -0.3 3,103,053 - -0.3 3,103,239 - Shewana3_2599 0.14 +0.7
Or see this region's nucleotide sequence