Experiment: Uridine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3180 and Pf1N1B4_3181 overlap by 4 nucleotides Pf1N1B4_3181 and Pf1N1B4_3182 are separated by 241 nucleotides
Pf1N1B4_3180 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 3,561,774 to 3,563,462
_3180
Pf1N1B4_3181 - mRNA 3-end processing factor, at 3,563,459 to 3,564,502
_3181
Pf1N1B4_3182 - Penicillin acylase (EC 3.5.1.11), at 3,564,744 to 3,567,194
_3182
Position (kb)
3563
3564
3565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3562.487 kb on + strand, within Pf1N1B4_3180 at 3562.488 kb on - strand, within Pf1N1B4_3180 at 3562.488 kb on - strand, within Pf1N1B4_3180 at 3562.488 kb on - strand, within Pf1N1B4_3180 at 3562.617 kb on - strand, within Pf1N1B4_3180 at 3562.668 kb on + strand, within Pf1N1B4_3180 at 3562.895 kb on - strand, within Pf1N1B4_3180 at 3562.931 kb on - strand, within Pf1N1B4_3180 at 3563.236 kb on + strand, within Pf1N1B4_3180 at 3563.237 kb on - strand, within Pf1N1B4_3180 at 3563.237 kb on - strand, within Pf1N1B4_3180 at 3563.341 kb on + strand at 3563.341 kb on + strand at 3563.341 kb on + strand at 3563.341 kb on + strand at 3563.342 kb on - strand at 3563.383 kb on + strand at 3563.383 kb on + strand at 3563.383 kb on + strand at 3563.383 kb on + strand at 3563.383 kb on + strand at 3563.383 kb on + strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.384 kb on - strand at 3563.440 kb on + strand at 3563.440 kb on + strand at 3563.440 kb on + strand at 3563.440 kb on + strand at 3563.441 kb on - strand at 3563.441 kb on - strand at 3563.441 kb on - strand at 3563.441 kb on - strand at 3563.441 kb on - strand at 3563.441 kb on - strand at 3563.489 kb on + strand at 3563.489 kb on + strand at 3563.490 kb on - strand at 3563.490 kb on - strand at 3563.490 kb on - strand at 3563.562 kb on + strand at 3563.701 kb on + strand, within Pf1N1B4_3181 at 3563.702 kb on - strand, within Pf1N1B4_3181 at 3563.742 kb on + strand, within Pf1N1B4_3181 at 3563.742 kb on + strand, within Pf1N1B4_3181 at 3563.742 kb on + strand, within Pf1N1B4_3181 at 3563.742 kb on + strand at 3563.743 kb on - strand, within Pf1N1B4_3181 at 3563.830 kb on + strand, within Pf1N1B4_3181 at 3563.844 kb on + strand, within Pf1N1B4_3181 at 3563.844 kb on + strand, within Pf1N1B4_3181 at 3563.844 kb on + strand, within Pf1N1B4_3181 at 3563.844 kb on + strand, within Pf1N1B4_3181 at 3563.845 kb on - strand, within Pf1N1B4_3181 at 3563.845 kb on - strand, within Pf1N1B4_3181 at 3563.845 kb on - strand, within Pf1N1B4_3181 at 3563.862 kb on + strand, within Pf1N1B4_3181 at 3563.862 kb on + strand at 3563.862 kb on + strand, within Pf1N1B4_3181 at 3563.862 kb on + strand at 3563.863 kb on - strand, within Pf1N1B4_3181 at 3563.863 kb on - strand, within Pf1N1B4_3181 at 3563.863 kb on - strand, within Pf1N1B4_3181 at 3563.863 kb on - strand, within Pf1N1B4_3181 at 3563.863 kb on - strand, within Pf1N1B4_3181 at 3563.912 kb on + strand, within Pf1N1B4_3181 at 3563.912 kb on + strand, within Pf1N1B4_3181 at 3563.912 kb on + strand at 3563.912 kb on + strand at 3563.912 kb on + strand at 3563.913 kb on - strand, within Pf1N1B4_3181 at 3563.913 kb on - strand, within Pf1N1B4_3181 at 3563.976 kb on + strand, within Pf1N1B4_3181 at 3563.976 kb on + strand, within Pf1N1B4_3181 at 3563.976 kb on + strand, within Pf1N1B4_3181 at 3563.977 kb on - strand, within Pf1N1B4_3181 at 3563.977 kb on - strand, within Pf1N1B4_3181 at 3563.977 kb on - strand, within Pf1N1B4_3181 at 3564.106 kb on + strand, within Pf1N1B4_3181 at 3564.106 kb on + strand, within Pf1N1B4_3181 at 3564.106 kb on + strand, within Pf1N1B4_3181 at 3564.159 kb on + strand, within Pf1N1B4_3181 at 3564.159 kb on + strand, within Pf1N1B4_3181 at 3564.159 kb on + strand, within Pf1N1B4_3181 at 3564.159 kb on + strand, within Pf1N1B4_3181 at 3564.204 kb on - strand, within Pf1N1B4_3181 at 3564.204 kb on - strand, within Pf1N1B4_3181 at 3564.204 kb on - strand, within Pf1N1B4_3181 at 3564.221 kb on + strand, within Pf1N1B4_3181 at 3564.221 kb on + strand, within Pf1N1B4_3181 at 3564.222 kb on - strand, within Pf1N1B4_3181 at 3564.222 kb on - strand, within Pf1N1B4_3181 at 3564.222 kb on - strand, within Pf1N1B4_3181 at 3564.251 kb on + strand, within Pf1N1B4_3181 at 3564.252 kb on - strand, within Pf1N1B4_3181 at 3564.252 kb on - strand, within Pf1N1B4_3181 at 3564.354 kb on + strand, within Pf1N1B4_3181 at 3564.354 kb on + strand, within Pf1N1B4_3181 at 3564.354 kb on + strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.355 kb on - strand, within Pf1N1B4_3181 at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.435 kb on + strand at 3564.436 kb on - strand at 3564.436 kb on - strand at 3564.456 kb on + strand at 3564.456 kb on + strand at 3564.456 kb on + strand at 3564.517 kb on + strand at 3564.517 kb on + strand at 3564.550 kb on - strand at 3564.550 kb on - strand at 3564.745 kb on + strand at 3564.745 kb on + strand at 3564.745 kb on + strand at 3564.745 kb on + strand at 3564.746 kb on - strand at 3564.746 kb on - strand at 3564.746 kb on - strand at 3564.746 kb on - strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.823 kb on + strand at 3564.824 kb on - strand at 3564.824 kb on - strand at 3564.824 kb on - strand at 3564.824 kb on - strand at 3564.824 kb on - strand at 3564.859 kb on + strand at 3564.860 kb on - strand at 3565.073 kb on - strand, within Pf1N1B4_3182 at 3565.360 kb on + strand, within Pf1N1B4_3182 at 3565.360 kb on + strand, within Pf1N1B4_3182 at 3565.360 kb on + strand, within Pf1N1B4_3182 at 3565.361 kb on - strand, within Pf1N1B4_3182 at 3565.361 kb on - strand, within Pf1N1B4_3182 at 3565.361 kb on - strand, within Pf1N1B4_3182 at 3565.418 kb on + strand, within Pf1N1B4_3182 at 3565.418 kb on + strand at 3565.418 kb on + strand, within Pf1N1B4_3182 at 3565.420 kb on + strand, within Pf1N1B4_3182 at 3565.420 kb on + strand, within Pf1N1B4_3182 at 3565.420 kb on + strand, within Pf1N1B4_3182 at 3565.420 kb on + strand, within Pf1N1B4_3182 at 3565.420 kb on + strand, within Pf1N1B4_3182 at 3565.421 kb on - strand, within Pf1N1B4_3182 at 3565.421 kb on - strand, within Pf1N1B4_3182
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (C) remove 3,562,487 + Pf1N1B4_3180 0.42 -0.3 3,562,488 - Pf1N1B4_3180 0.42 +0.7 3,562,488 - Pf1N1B4_3180 0.42 +0.6 3,562,488 - Pf1N1B4_3180 0.42 -0.0 3,562,617 - Pf1N1B4_3180 0.50 -0.0 3,562,668 + Pf1N1B4_3180 0.53 +0.8 3,562,895 - Pf1N1B4_3180 0.66 +1.6 3,562,931 - Pf1N1B4_3180 0.69 +0.9 3,563,236 + Pf1N1B4_3180 0.87 +0.6 3,563,237 - Pf1N1B4_3180 0.87 -1.6 3,563,237 - Pf1N1B4_3180 0.87 -1.0 3,563,341 + +0.2 3,563,341 + +0.3 3,563,341 + +0.0 3,563,341 + -0.4 3,563,342 - +0.4 3,563,383 + -0.6 3,563,383 + -0.2 3,563,383 + +2.6 3,563,383 + +1.4 3,563,383 + -0.2 3,563,383 + -0.1 3,563,384 - -0.7 3,563,384 - -0.4 3,563,384 - +1.2 3,563,384 - +0.6 3,563,384 - +2.0 3,563,384 - +0.5 3,563,384 - +1.0 3,563,384 - +1.8 3,563,384 - -0.3 3,563,384 - +0.0 3,563,384 - +0.8 3,563,440 + +0.2 3,563,440 + -0.7 3,563,440 + -1.2 3,563,440 + +0.0 3,563,441 - +2.6 3,563,441 - +2.3 3,563,441 - +0.6 3,563,441 - +0.6 3,563,441 - +1.3 3,563,441 - -0.4 3,563,489 + -1.6 3,563,489 + +0.0 3,563,490 - -0.8 3,563,490 - +0.4 3,563,490 - +0.6 3,563,562 + +0.0 3,563,701 + Pf1N1B4_3181 0.23 -0.7 3,563,702 - Pf1N1B4_3181 0.23 +0.3 3,563,742 + Pf1N1B4_3181 0.27 +0.5 3,563,742 + Pf1N1B4_3181 0.27 -0.4 3,563,742 + Pf1N1B4_3181 0.27 +0.0 3,563,742 + +0.0 3,563,743 - Pf1N1B4_3181 0.27 +0.0 3,563,830 + Pf1N1B4_3181 0.36 -2.0 3,563,844 + Pf1N1B4_3181 0.37 -2.0 3,563,844 + Pf1N1B4_3181 0.37 +0.4 3,563,844 + Pf1N1B4_3181 0.37 +0.3 3,563,844 + Pf1N1B4_3181 0.37 +0.7 3,563,845 - Pf1N1B4_3181 0.37 +1.6 3,563,845 - Pf1N1B4_3181 0.37 -0.3 3,563,845 - Pf1N1B4_3181 0.37 -2.8 3,563,862 + Pf1N1B4_3181 0.39 +1.7 3,563,862 + +0.0 3,563,862 + Pf1N1B4_3181 0.39 -2.3 3,563,862 + +1.6 3,563,863 - Pf1N1B4_3181 0.39 -0.2 3,563,863 - Pf1N1B4_3181 0.39 +0.8 3,563,863 - Pf1N1B4_3181 0.39 +0.3 3,563,863 - Pf1N1B4_3181 0.39 +2.0 3,563,863 - Pf1N1B4_3181 0.39 -0.2 3,563,912 + Pf1N1B4_3181 0.43 +0.6 3,563,912 + Pf1N1B4_3181 0.43 -0.3 3,563,912 + +0.0 3,563,912 + +0.0 3,563,912 + +2.3 3,563,913 - Pf1N1B4_3181 0.43 +0.4 3,563,913 - Pf1N1B4_3181 0.43 +0.3 3,563,976 + Pf1N1B4_3181 0.50 -0.8 3,563,976 + Pf1N1B4_3181 0.50 +0.4 3,563,976 + Pf1N1B4_3181 0.50 +1.7 3,563,977 - Pf1N1B4_3181 0.50 +0.2 3,563,977 - Pf1N1B4_3181 0.50 +0.3 3,563,977 - Pf1N1B4_3181 0.50 -0.4 3,564,106 + Pf1N1B4_3181 0.62 +1.0 3,564,106 + Pf1N1B4_3181 0.62 +0.4 3,564,106 + Pf1N1B4_3181 0.62 +0.7 3,564,159 + Pf1N1B4_3181 0.67 -0.2 3,564,159 + Pf1N1B4_3181 0.67 +1.0 3,564,159 + Pf1N1B4_3181 0.67 +0.7 3,564,159 + Pf1N1B4_3181 0.67 +0.0 3,564,204 - Pf1N1B4_3181 0.71 +0.0 3,564,204 - Pf1N1B4_3181 0.71 +0.0 3,564,204 - Pf1N1B4_3181 0.71 +0.0 3,564,221 + Pf1N1B4_3181 0.73 +0.8 3,564,221 + Pf1N1B4_3181 0.73 +0.6 3,564,222 - Pf1N1B4_3181 0.73 -1.0 3,564,222 - Pf1N1B4_3181 0.73 +1.2 3,564,222 - Pf1N1B4_3181 0.73 -0.4 3,564,251 + Pf1N1B4_3181 0.76 +0.0 3,564,252 - Pf1N1B4_3181 0.76 -0.3 3,564,252 - Pf1N1B4_3181 0.76 -0.6 3,564,354 + Pf1N1B4_3181 0.86 +1.3 3,564,354 + Pf1N1B4_3181 0.86 +0.4 3,564,354 + Pf1N1B4_3181 0.86 +0.0 3,564,355 - Pf1N1B4_3181 0.86 +0.5 3,564,355 - Pf1N1B4_3181 0.86 +1.0 3,564,355 - Pf1N1B4_3181 0.86 +1.0 3,564,355 - Pf1N1B4_3181 0.86 +0.0 3,564,355 - Pf1N1B4_3181 0.86 +0.0 3,564,355 - Pf1N1B4_3181 0.86 -1.6 3,564,435 + +1.0 3,564,435 + +0.7 3,564,435 + -0.7 3,564,435 + -0.3 3,564,435 + +1.5 3,564,435 + +0.6 3,564,435 + -1.6 3,564,435 + -0.7 3,564,435 + +1.3 3,564,435 + +0.0 3,564,436 - +0.3 3,564,436 - +0.0 3,564,456 + -1.0 3,564,456 + +0.8 3,564,456 + -1.6 3,564,517 + +0.5 3,564,517 + -1.6 3,564,550 - +1.3 3,564,550 - +0.0 3,564,745 + -2.6 3,564,745 + -0.4 3,564,745 + +2.2 3,564,745 + +0.6 3,564,746 - +1.0 3,564,746 - +2.6 3,564,746 - +0.1 3,564,746 - -0.4 3,564,823 + +0.6 3,564,823 + +0.1 3,564,823 + +2.0 3,564,823 + +0.0 3,564,823 + +0.0 3,564,823 + -1.0 3,564,823 + +2.3 3,564,823 + +2.8 3,564,823 + -1.0 3,564,824 - -1.6 3,564,824 - +0.0 3,564,824 - +0.5 3,564,824 - +0.0 3,564,824 - +0.3 3,564,859 + -0.2 3,564,860 - +0.0 3,565,073 - Pf1N1B4_3182 0.13 -2.3 3,565,360 + Pf1N1B4_3182 0.25 -0.1 3,565,360 + Pf1N1B4_3182 0.25 -0.7 3,565,360 + Pf1N1B4_3182 0.25 +2.3 3,565,361 - Pf1N1B4_3182 0.25 -0.3 3,565,361 - Pf1N1B4_3182 0.25 -0.4 3,565,361 - Pf1N1B4_3182 0.25 -1.0 3,565,418 + Pf1N1B4_3182 0.27 +0.0 3,565,418 + -0.6 3,565,418 + Pf1N1B4_3182 0.27 -2.0 3,565,420 + Pf1N1B4_3182 0.28 -1.7 3,565,420 + Pf1N1B4_3182 0.28 +0.3 3,565,420 + Pf1N1B4_3182 0.28 +0.4 3,565,420 + Pf1N1B4_3182 0.28 -2.8 3,565,420 + Pf1N1B4_3182 0.28 +1.3 3,565,421 - Pf1N1B4_3182 0.28 -1.0 3,565,421 - Pf1N1B4_3182 0.28 -0.6
Or see this region's nucleotide sequence