Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06559 and H281DRAFT_06560 are separated by 85 nucleotides H281DRAFT_06560 and H281DRAFT_06561 overlap by 10 nucleotides H281DRAFT_06561 and H281DRAFT_06562 are separated by 90 nucleotides H281DRAFT_06562 and H281DRAFT_06563 are separated by 236 nucleotides H281DRAFT_06563 and H281DRAFT_06564 are separated by 136 nucleotides
H281DRAFT_06559: H281DRAFT_06559 - hypothetical protein, at 1,586 to 1,891
_06559
H281DRAFT_06560: H281DRAFT_06560 - hypothetical protein, at 1,977 to 2,675
_06560
H281DRAFT_06561: H281DRAFT_06561 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,666 to 3,037
_06561
H281DRAFT_06562: H281DRAFT_06562 - hypothetical protein, at 3,128 to 3,346
_06562
H281DRAFT_06563: H281DRAFT_06563 - hypothetical protein, at 3,583 to 3,846
_06563
H281DRAFT_06564: H281DRAFT_06564 - hypothetical protein, at 3,983 to 4,183
_06564
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.995 kb on - strand at 2.147 kb on - strand, within H281DRAFT_06560 at 2.315 kb on - strand, within H281DRAFT_06560 at 2.376 kb on + strand, within H281DRAFT_06560 at 2.613 kb on + strand at 2.613 kb on + strand at 2.613 kb on + strand at 2.614 kb on - strand at 2.642 kb on + strand at 2.674 kb on - strand at 2.708 kb on + strand, within H281DRAFT_06561 at 2.709 kb on - strand, within H281DRAFT_06561 at 2.787 kb on + strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.788 kb on - strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.891 kb on + strand, within H281DRAFT_06561 at 2.892 kb on - strand, within H281DRAFT_06561 at 2.917 kb on + strand, within H281DRAFT_06561 at 2.918 kb on - strand at 2.918 kb on - strand, within H281DRAFT_06561 at 2.918 kb on - strand, within H281DRAFT_06561 at 2.923 kb on + strand, within H281DRAFT_06561 at 2.924 kb on - strand, within H281DRAFT_06561 at 3.012 kb on + strand at 3.012 kb on + strand at 3.012 kb on + strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.013 kb on - strand at 3.095 kb on + strand at 3.095 kb on + strand at 3.141 kb on + strand at 3.142 kb on - strand at 3.250 kb on + strand, within H281DRAFT_06562 at 3.334 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.346 kb on + strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.347 kb on - strand at 3.348 kb on + strand at 3.349 kb on - strand at 3.349 kb on - strand at 3.356 kb on + strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.357 kb on - strand at 3.375 kb on + strand at 3.375 kb on + strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.376 kb on - strand at 3.430 kb on - strand at 3.435 kb on + strand at 3.436 kb on - strand at 3.558 kb on + strand at 3.559 kb on - strand at 3.563 kb on + strand at 3.563 kb on + strand at 3.568 kb on + strand at 3.568 kb on + strand at 3.682 kb on + strand, within H281DRAFT_06563 at 3.802 kb on + strand, within H281DRAFT_06563 at 3.803 kb on - strand, within H281DRAFT_06563 at 3.804 kb on + strand, within H281DRAFT_06563 at 3.805 kb on - strand, within H281DRAFT_06563 at 3.805 kb on - strand, within H281DRAFT_06563 at 3.873 kb on + strand at 3.873 kb on + strand at 3.874 kb on - strand at 3.940 kb on - strand at 3.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 1,995 - +0.8 2,147 - H281DRAFT_06560 0.24 +1.6 2,315 - H281DRAFT_06560 0.48 -0.1 2,376 + H281DRAFT_06560 0.57 -1.2 2,613 + -0.2 2,613 + +0.3 2,613 + +0.3 2,614 - +0.6 2,642 + -0.7 2,674 - -0.6 2,708 + H281DRAFT_06561 0.11 +0.9 2,709 - H281DRAFT_06561 0.12 -0.6 2,787 + H281DRAFT_06561 0.33 +0.8 2,788 - H281DRAFT_06561 0.33 -0.3 2,788 - H281DRAFT_06561 0.33 -1.1 2,788 - H281DRAFT_06561 0.33 +0.8 2,788 - H281DRAFT_06561 0.33 -0.2 2,891 + H281DRAFT_06561 0.60 +0.4 2,891 + H281DRAFT_06561 0.60 -0.4 2,892 - H281DRAFT_06561 0.61 +0.2 2,917 + H281DRAFT_06561 0.67 +0.3 2,918 - +0.3 2,918 - H281DRAFT_06561 0.68 +0.4 2,918 - H281DRAFT_06561 0.68 +1.9 2,923 + H281DRAFT_06561 0.69 -0.5 2,924 - H281DRAFT_06561 0.69 +1.4 3,012 + -0.7 3,012 + +1.7 3,012 + +1.6 3,013 - -0.1 3,013 - +0.9 3,013 - +0.9 3,013 - +2.5 3,095 + +0.1 3,095 + +0.9 3,141 + -0.3 3,142 - -0.1 3,250 + H281DRAFT_06562 0.56 -0.8 3,334 + +0.1 3,346 + +0.3 3,346 + -0.1 3,346 + -0.6 3,346 + +0.2 3,346 + -0.9 3,346 + -0.9 3,346 + -0.3 3,346 + +0.3 3,347 - +0.3 3,347 - +2.4 3,347 - -0.4 3,347 - +0.7 3,347 - +0.5 3,348 + +0.2 3,349 - +0.4 3,349 - +0.1 3,356 + -2.3 3,357 - +0.7 3,357 - -0.4 3,357 - +0.3 3,375 + +0.3 3,375 + -0.5 3,376 - -2.1 3,376 - -0.2 3,376 - -1.3 3,430 - -0.1 3,435 + +0.5 3,436 - -1.3 3,558 + +0.8 3,559 - -0.5 3,563 + -0.7 3,563 + +0.0 3,568 + +0.6 3,568 + -0.9 3,682 + H281DRAFT_06563 0.38 -0.7 3,802 + H281DRAFT_06563 0.83 -0.3 3,803 - H281DRAFT_06563 0.83 -1.9 3,804 + H281DRAFT_06563 0.84 -0.4 3,805 - H281DRAFT_06563 0.84 -1.7 3,805 - H281DRAFT_06563 0.84 -1.4 3,873 + +1.3 3,873 + +1.6 3,874 - -1.4 3,940 - -0.7 3,971 + -0.0
Or see this region's nucleotide sequence