Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06447 and H281DRAFT_06448 are separated by 94 nucleotides
H281DRAFT_06447: H281DRAFT_06447 - protein PsiE, at 52,737 to 53,195
_06447
H281DRAFT_06448: H281DRAFT_06448 - transcriptional regulator, LysR family, at 53,290 to 54,207
_06448
Position (kb) 53
54
55 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 52.391 kb on + strand at 52.391 kb on + strand at 52.398 kb on + strand at 52.398 kb on + strand at 52.398 kb on + strand at 52.399 kb on - strand at 52.399 kb on - strand at 52.399 kb on - strand at 52.558 kb on + strand at 52.558 kb on + strand at 52.567 kb on + strand at 52.567 kb on + strand at 52.738 kb on + strand at 52.738 kb on + strand at 52.738 kb on + strand at 52.739 kb on - strand at 52.816 kb on - strand, within H281DRAFT_06447 at 52.816 kb on - strand, within H281DRAFT_06447 at 52.845 kb on + strand, within H281DRAFT_06447 at 52.846 kb on - strand, within H281DRAFT_06447 at 52.846 kb on - strand, within H281DRAFT_06447 at 52.859 kb on + strand, within H281DRAFT_06447 at 52.860 kb on - strand, within H281DRAFT_06447 at 52.863 kb on - strand, within H281DRAFT_06447 at 52.868 kb on + strand, within H281DRAFT_06447 at 52.931 kb on + strand, within H281DRAFT_06447 at 52.968 kb on + strand, within H281DRAFT_06447 at 52.973 kb on - strand, within H281DRAFT_06447 at 53.003 kb on - strand, within H281DRAFT_06447 at 53.004 kb on + strand, within H281DRAFT_06447 at 53.005 kb on - strand, within H281DRAFT_06447 at 53.005 kb on - strand, within H281DRAFT_06447 at 53.005 kb on - strand, within H281DRAFT_06447 at 53.019 kb on + strand, within H281DRAFT_06447 at 53.020 kb on - strand, within H281DRAFT_06447 at 53.130 kb on + strand, within H281DRAFT_06447 at 53.291 kb on + strand at 53.529 kb on - strand, within H281DRAFT_06448 at 53.672 kb on - strand, within H281DRAFT_06448 at 53.696 kb on + strand, within H281DRAFT_06448 at 53.747 kb on - strand, within H281DRAFT_06448 at 53.980 kb on + strand, within H281DRAFT_06448 at 53.981 kb on - strand, within H281DRAFT_06448 at 54.004 kb on - strand at 54.038 kb on + strand, within H281DRAFT_06448 at 54.039 kb on - strand, within H281DRAFT_06448 at 54.081 kb on - strand, within H281DRAFT_06448 at 54.081 kb on - strand, within H281DRAFT_06448 at 54.154 kb on - strand at 54.179 kb on + strand at 54.179 kb on + strand at 54.179 kb on + strand at 54.180 kb on - strand at 54.180 kb on - strand at 54.181 kb on + strand at 54.181 kb on + strand at 54.181 kb on + strand at 54.182 kb on - strand at 54.182 kb on - strand at 54.182 kb on - strand at 54.182 kb on - strand at 54.182 kb on - strand at 54.210 kb on + strand at 54.361 kb on + strand at 54.361 kb on + strand at 54.361 kb on + strand at 54.361 kb on + strand at 54.361 kb on + strand at 54.362 kb on - strand at 54.362 kb on - strand at 54.362 kb on - strand at 54.362 kb on - strand at 54.362 kb on - strand at 54.431 kb on + strand at 54.431 kb on + strand at 54.432 kb on - strand at 54.441 kb on + strand at 54.613 kb on - strand at 54.756 kb on - strand at 54.784 kb on + strand at 54.784 kb on + strand at 54.785 kb on - strand at 54.817 kb on + strand at 54.817 kb on + strand at 55.168 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 52,391 + -0.4 52,391 + -1.0 52,398 + -0.9 52,398 + -0.6 52,398 + -0.0 52,399 - +0.3 52,399 - +0.6 52,399 - -0.3 52,558 + +1.0 52,558 + +0.2 52,567 + +0.6 52,567 + -0.6 52,738 + -0.6 52,738 + -0.7 52,738 + +0.5 52,739 - +0.2 52,816 - H281DRAFT_06447 0.17 -0.7 52,816 - H281DRAFT_06447 0.17 -0.4 52,845 + H281DRAFT_06447 0.24 -0.3 52,846 - H281DRAFT_06447 0.24 -0.2 52,846 - H281DRAFT_06447 0.24 +0.2 52,859 + H281DRAFT_06447 0.27 +0.1 52,860 - H281DRAFT_06447 0.27 +0.3 52,863 - H281DRAFT_06447 0.27 -0.3 52,868 + H281DRAFT_06447 0.29 +0.1 52,931 + H281DRAFT_06447 0.42 -0.3 52,968 + H281DRAFT_06447 0.50 +0.4 52,973 - H281DRAFT_06447 0.51 -0.8 53,003 - H281DRAFT_06447 0.58 +0.6 53,004 + H281DRAFT_06447 0.58 +0.1 53,005 - H281DRAFT_06447 0.58 -0.5 53,005 - H281DRAFT_06447 0.58 +0.9 53,005 - H281DRAFT_06447 0.58 +0.0 53,019 + H281DRAFT_06447 0.61 +0.9 53,020 - H281DRAFT_06447 0.62 +0.1 53,130 + H281DRAFT_06447 0.86 -0.7 53,291 + +0.7 53,529 - H281DRAFT_06448 0.26 -0.7 53,672 - H281DRAFT_06448 0.42 -0.9 53,696 + H281DRAFT_06448 0.44 -0.3 53,747 - H281DRAFT_06448 0.50 +0.2 53,980 + H281DRAFT_06448 0.75 +0.1 53,981 - H281DRAFT_06448 0.75 -0.3 54,004 - +1.9 54,038 + H281DRAFT_06448 0.81 +0.3 54,039 - H281DRAFT_06448 0.82 +0.8 54,081 - H281DRAFT_06448 0.86 -0.3 54,081 - H281DRAFT_06448 0.86 +0.8 54,154 - -0.0 54,179 + +1.9 54,179 + -0.1 54,179 + +0.3 54,180 - -0.7 54,180 - +0.3 54,181 + -0.5 54,181 + -0.5 54,181 + +0.8 54,182 - +0.2 54,182 - +3.6 54,182 - +1.9 54,182 - +0.2 54,182 - -0.5 54,210 + +0.7 54,361 + -0.2 54,361 + -1.1 54,361 + +0.2 54,361 + +1.7 54,361 + -1.3 54,362 - -2.8 54,362 - +0.0 54,362 - +0.9 54,362 - +0.7 54,362 - -0.7 54,431 + +0.9 54,431 + +0.2 54,432 - -0.9 54,441 + +0.7 54,613 - -0.6 54,756 - +0.1 54,784 + -0.1 54,784 + -2.1 54,785 - +1.2 54,817 + +0.2 54,817 + +0.4 55,168 + +1.0
Or see this region's nucleotide sequence