Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06248 and H281DRAFT_06249 are separated by 163 nucleotides H281DRAFT_06249 and H281DRAFT_06250 are separated by 40 nucleotides H281DRAFT_06250 and H281DRAFT_06251 overlap by 4 nucleotides
H281DRAFT_06248: H281DRAFT_06248 - protein translocase subunit secA, at 28,863 to 31,673
_06248
H281DRAFT_06249: H281DRAFT_06249 - glutamate N-acetyltransferase, at 31,837 to 33,078
_06249
H281DRAFT_06250: H281DRAFT_06250 - hypothetical protein, at 33,119 to 33,988
_06250
H281DRAFT_06251: H281DRAFT_06251 - 8-oxo-dGTPase (EC 3.6.1.55), at 33,985 to 34,407
_06251
Position (kb)
31
32
33
34 Strain fitness (log2 ratio)
-1
0
1
2 at 31.644 kb on + strand at 31.644 kb on + strand at 31.777 kb on - strand at 31.777 kb on - strand at 32.291 kb on - strand, within H281DRAFT_06249 at 32.535 kb on + strand, within H281DRAFT_06249 at 32.535 kb on + strand, within H281DRAFT_06249 at 32.717 kb on + strand, within H281DRAFT_06249 at 32.717 kb on + strand, within H281DRAFT_06249 at 32.718 kb on - strand, within H281DRAFT_06249 at 32.718 kb on - strand, within H281DRAFT_06249 at 32.797 kb on + strand, within H281DRAFT_06249 at 32.797 kb on + strand, within H281DRAFT_06249 at 32.798 kb on - strand, within H281DRAFT_06249 at 32.798 kb on - strand, within H281DRAFT_06249 at 32.885 kb on - strand, within H281DRAFT_06249 at 33.046 kb on + strand at 33.046 kb on + strand at 33.047 kb on - strand at 33.047 kb on - strand at 33.047 kb on - strand at 33.047 kb on - strand at 33.077 kb on - strand at 33.118 kb on + strand at 33.119 kb on - strand at 33.258 kb on - strand, within H281DRAFT_06250 at 33.443 kb on + strand, within H281DRAFT_06250 at 33.587 kb on + strand, within H281DRAFT_06250 at 33.587 kb on + strand, within H281DRAFT_06250 at 33.650 kb on + strand, within H281DRAFT_06250 at 33.650 kb on + strand, within H281DRAFT_06250 at 33.687 kb on - strand, within H281DRAFT_06250 at 33.687 kb on - strand, within H281DRAFT_06250 at 33.707 kb on + strand, within H281DRAFT_06250 at 33.707 kb on + strand, within H281DRAFT_06250 at 33.707 kb on + strand, within H281DRAFT_06250 at 33.707 kb on + strand, within H281DRAFT_06250 at 33.708 kb on - strand, within H281DRAFT_06250 at 33.708 kb on - strand, within H281DRAFT_06250 at 33.708 kb on - strand, within H281DRAFT_06250 at 33.708 kb on - strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.709 kb on + strand, within H281DRAFT_06250 at 33.710 kb on - strand, within H281DRAFT_06250 at 33.710 kb on - strand, within H281DRAFT_06250 at 33.710 kb on - strand, within H281DRAFT_06250 at 33.710 kb on - strand, within H281DRAFT_06250 at 33.800 kb on + strand, within H281DRAFT_06250 at 33.822 kb on - strand, within H281DRAFT_06250 at 33.822 kb on - strand, within H281DRAFT_06250 at 33.822 kb on - strand, within H281DRAFT_06250
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 31,644 + +0.5 31,644 + -0.6 31,777 - +0.9 31,777 - +1.0 32,291 - H281DRAFT_06249 0.37 +0.7 32,535 + H281DRAFT_06249 0.56 +0.3 32,535 + H281DRAFT_06249 0.56 +2.2 32,717 + H281DRAFT_06249 0.71 +1.3 32,717 + H281DRAFT_06249 0.71 +1.0 32,718 - H281DRAFT_06249 0.71 -0.0 32,718 - H281DRAFT_06249 0.71 +0.3 32,797 + H281DRAFT_06249 0.77 +0.8 32,797 + H281DRAFT_06249 0.77 +0.9 32,798 - H281DRAFT_06249 0.77 +0.4 32,798 - H281DRAFT_06249 0.77 -0.2 32,885 - H281DRAFT_06249 0.84 +0.4 33,046 + +0.3 33,046 + +0.6 33,047 - +0.0 33,047 - +0.2 33,047 - +0.9 33,047 - -0.1 33,077 - -0.1 33,118 + -0.2 33,119 - +0.7 33,258 - H281DRAFT_06250 0.16 -0.7 33,443 + H281DRAFT_06250 0.37 +0.3 33,587 + H281DRAFT_06250 0.54 +0.2 33,587 + H281DRAFT_06250 0.54 -0.0 33,650 + H281DRAFT_06250 0.61 +0.5 33,650 + H281DRAFT_06250 0.61 -0.4 33,687 - H281DRAFT_06250 0.65 -0.0 33,687 - H281DRAFT_06250 0.65 -0.1 33,707 + H281DRAFT_06250 0.68 +0.7 33,707 + H281DRAFT_06250 0.68 +0.4 33,707 + H281DRAFT_06250 0.68 +0.9 33,707 + H281DRAFT_06250 0.68 +0.5 33,708 - H281DRAFT_06250 0.68 -0.6 33,708 - H281DRAFT_06250 0.68 +1.4 33,708 - H281DRAFT_06250 0.68 -0.2 33,708 - H281DRAFT_06250 0.68 -1.0 33,709 + H281DRAFT_06250 0.68 +0.0 33,709 + H281DRAFT_06250 0.68 -1.1 33,709 + H281DRAFT_06250 0.68 -0.5 33,709 + H281DRAFT_06250 0.68 +0.6 33,709 + H281DRAFT_06250 0.68 -0.0 33,709 + H281DRAFT_06250 0.68 +0.3 33,710 - H281DRAFT_06250 0.68 -0.3 33,710 - H281DRAFT_06250 0.68 -0.4 33,710 - H281DRAFT_06250 0.68 -0.1 33,710 - H281DRAFT_06250 0.68 +0.2 33,800 + H281DRAFT_06250 0.78 +0.3 33,822 - H281DRAFT_06250 0.81 +0.1 33,822 - H281DRAFT_06250 0.81 +0.2 33,822 - H281DRAFT_06250 0.81 -0.5
Or see this region's nucleotide sequence