Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05808 and H281DRAFT_05809 are separated by 288 nucleotides H281DRAFT_05809 and H281DRAFT_05810 overlap by 4 nucleotides
H281DRAFT_05808: H281DRAFT_05808 - Tfp pilus assembly protein PilF, at 84,247 to 85,980
_05808
H281DRAFT_05809: H281DRAFT_05809 - Uncharacterized membrane protein, at 86,269 to 87,225
_05809
H281DRAFT_05810: H281DRAFT_05810 - Uncharacterized membrane protein, at 87,222 to 87,971
_05810
Position (kb) 86
87
88 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 85.699 kb on + strand, within H281DRAFT_05808 at 85.699 kb on + strand, within H281DRAFT_05808 at 85.718 kb on + strand, within H281DRAFT_05808 at 85.856 kb on + strand at 85.856 kb on + strand at 85.922 kb on + strand at 85.923 kb on - strand at 85.990 kb on - strand at 86.103 kb on + strand at 86.123 kb on + strand at 86.124 kb on - strand at 86.259 kb on - strand at 86.328 kb on + strand at 86.333 kb on - strand at 86.582 kb on + strand, within H281DRAFT_05809 at 86.583 kb on - strand, within H281DRAFT_05809 at 86.583 kb on - strand, within H281DRAFT_05809 at 86.620 kb on - strand, within H281DRAFT_05809 at 86.621 kb on + strand, within H281DRAFT_05809 at 87.091 kb on + strand, within H281DRAFT_05809 at 87.207 kb on - strand at 87.210 kb on + strand at 87.211 kb on - strand at 87.211 kb on - strand at 87.323 kb on - strand, within H281DRAFT_05810 at 87.425 kb on + strand, within H281DRAFT_05810 at 87.804 kb on - strand, within H281DRAFT_05810 at 87.952 kb on - strand at 87.952 kb on - strand at 88.082 kb on - strand at 88.082 kb on - strand at 88.208 kb on + strand at 88.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 85,699 + H281DRAFT_05808 0.84 +0.1 85,699 + H281DRAFT_05808 0.84 -1.4 85,718 + H281DRAFT_05808 0.85 -0.1 85,856 + +0.3 85,856 + +0.8 85,922 + +0.7 85,923 - +0.4 85,990 - +0.1 86,103 + -1.0 86,123 + -1.3 86,124 - -0.6 86,259 - +1.0 86,328 + +0.5 86,333 - -0.6 86,582 + H281DRAFT_05809 0.33 +1.3 86,583 - H281DRAFT_05809 0.33 +0.8 86,583 - H281DRAFT_05809 0.33 +0.3 86,620 - H281DRAFT_05809 0.37 +0.7 86,621 + H281DRAFT_05809 0.37 +0.8 87,091 + H281DRAFT_05809 0.86 +0.3 87,207 - +0.1 87,210 + +2.2 87,211 - +0.3 87,211 - +0.9 87,323 - H281DRAFT_05810 0.13 +0.1 87,425 + H281DRAFT_05810 0.27 +0.8 87,804 - H281DRAFT_05810 0.78 +0.9 87,952 - +0.4 87,952 - +0.6 88,082 - -0.4 88,082 - -4.0 88,208 + +0.3 88,209 - +0.4
Or see this region's nucleotide sequence