Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05723 and H281DRAFT_05724 are separated by 121 nucleotides H281DRAFT_05724 and H281DRAFT_05725 are separated by 131 nucleotides
H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) , at 141,891 to 143,093
_05723
H281DRAFT_05724: H281DRAFT_05724 - 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data) , at 143,215 to 144,915
_05724
H281DRAFT_05725: H281DRAFT_05725 - 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) (from data) , at 145,047 to 145,823
_05725
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.276 kb on + strand, within H281DRAFT_05723 at 142.353 kb on + strand, within H281DRAFT_05723 at 142.354 kb on - strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.374 kb on + strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.375 kb on - strand, within H281DRAFT_05723 at 142.837 kb on - strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.933 kb on + strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.934 kb on - strand, within H281DRAFT_05723 at 142.974 kb on - strand at 143.078 kb on + strand at 143.078 kb on + strand at 143.080 kb on + strand at 143.081 kb on - strand at 143.094 kb on - strand at 143.094 kb on - strand at 143.105 kb on + strand at 143.144 kb on + strand at 143.144 kb on + strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.145 kb on - strand at 143.184 kb on + strand at 143.185 kb on - strand at 143.185 kb on - strand at 143.207 kb on + strand at 143.207 kb on + strand at 143.225 kb on - strand at 143.401 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.867 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724 at 143.996 kb on + strand, within H281DRAFT_05724 at 144.127 kb on + strand, within H281DRAFT_05724 at 144.166 kb on + strand, within H281DRAFT_05724 at 144.166 kb on + strand, within H281DRAFT_05724 at 144.431 kb on + strand, within H281DRAFT_05724 at 144.431 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.522 kb on + strand, within H281DRAFT_05724 at 144.660 kb on + strand, within H281DRAFT_05724 at 144.697 kb on + strand, within H281DRAFT_05724 at 144.698 kb on - strand, within H281DRAFT_05724 at 144.933 kb on - strand at 144.954 kb on - strand at 144.954 kb on - strand at 144.957 kb on - strand at 144.957 kb on - strand at 144.993 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 144.996 kb on - strand at 145.036 kb on + strand at 145.036 kb on + strand at 145.037 kb on - strand at 145.053 kb on + strand at 145.264 kb on - strand, within H281DRAFT_05725 at 145.264 kb on - strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand, within H281DRAFT_05725 at 145.269 kb on + strand at 145.270 kb on - strand, within H281DRAFT_05725 at 145.270 kb on - strand, within H281DRAFT_05725 at 145.271 kb on + strand, within H281DRAFT_05725 at 145.272 kb on - strand, within H281DRAFT_05725 at 145.272 kb on - strand, within H281DRAFT_05725 at 145.281 kb on + strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.282 kb on - strand, within H281DRAFT_05725 at 145.293 kb on + strand at 145.293 kb on + strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.294 kb on - strand, within H281DRAFT_05725 at 145.304 kb on + strand, within H281DRAFT_05725 at 145.304 kb on + strand, within H281DRAFT_05725 at 145.305 kb on - strand, within H281DRAFT_05725 at 145.331 kb on + strand, within H281DRAFT_05725 at 145.331 kb on + strand, within H281DRAFT_05725 at 145.332 kb on - strand, within H281DRAFT_05725 at 145.509 kb on - strand, within H281DRAFT_05725 at 145.542 kb on - strand, within H281DRAFT_05725 at 145.542 kb on - strand, within H281DRAFT_05725 at 145.635 kb on - strand, within H281DRAFT_05725 at 145.824 kb on + strand at 145.824 kb on + strand at 145.824 kb on + strand at 145.824 kb on + strand at 145.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 142,276 + H281DRAFT_05723 0.32 -1.7 142,276 + H281DRAFT_05723 0.32 +2.2 142,353 + H281DRAFT_05723 0.38 -0.1 142,354 - H281DRAFT_05723 0.38 +0.3 142,374 + H281DRAFT_05723 0.40 +0.0 142,374 + H281DRAFT_05723 0.40 +1.3 142,374 + H281DRAFT_05723 0.40 +0.2 142,375 - H281DRAFT_05723 0.40 +1.0 142,375 - H281DRAFT_05723 0.40 +0.4 142,375 - H281DRAFT_05723 0.40 -0.4 142,375 - H281DRAFT_05723 0.40 -0.2 142,375 - H281DRAFT_05723 0.40 +0.4 142,837 - H281DRAFT_05723 0.79 -0.0 142,933 + H281DRAFT_05723 0.87 -1.3 142,933 + H281DRAFT_05723 0.87 -0.4 142,934 - H281DRAFT_05723 0.87 -0.0 142,934 - H281DRAFT_05723 0.87 +0.0 142,934 - H281DRAFT_05723 0.87 +0.6 142,974 - +0.3 143,078 + +0.6 143,078 + +1.9 143,080 + -1.5 143,081 - +0.1 143,094 - -0.1 143,094 - +2.6 143,105 + +0.2 143,144 + +0.8 143,144 + +0.8 143,145 - +0.3 143,145 - +0.1 143,145 - +0.4 143,145 - -1.5 143,145 - +0.1 143,145 - -0.3 143,145 - +0.4 143,145 - -1.0 143,145 - -0.5 143,184 + +0.3 143,185 - +0.2 143,185 - +0.7 143,207 + +0.7 143,207 + +0.2 143,225 - +0.0 143,401 + H281DRAFT_05724 0.11 -0.7 143,867 + H281DRAFT_05724 0.38 +0.9 143,867 + H281DRAFT_05724 0.38 +1.4 143,867 + H281DRAFT_05724 0.38 +2.0 143,867 + H281DRAFT_05724 0.38 +0.5 143,867 + H281DRAFT_05724 0.38 +0.5 143,996 + H281DRAFT_05724 0.46 +1.2 143,996 + H281DRAFT_05724 0.46 -0.1 144,127 + H281DRAFT_05724 0.54 +0.8 144,166 + H281DRAFT_05724 0.56 +0.3 144,166 + H281DRAFT_05724 0.56 +2.2 144,431 + H281DRAFT_05724 0.71 +0.3 144,431 + H281DRAFT_05724 0.71 +2.1 144,522 + H281DRAFT_05724 0.77 +1.0 144,522 + H281DRAFT_05724 0.77 +0.6 144,522 + H281DRAFT_05724 0.77 +0.8 144,660 + H281DRAFT_05724 0.85 +0.5 144,697 + H281DRAFT_05724 0.87 +1.8 144,698 - H281DRAFT_05724 0.87 +0.6 144,933 - +0.5 144,954 - +2.2 144,954 - +0.4 144,957 - +0.1 144,957 - -0.1 144,993 - -2.1 144,996 - +0.8 144,996 - -0.3 144,996 - -0.7 144,996 - +1.1 144,996 - -1.0 144,996 - +0.4 145,036 + +0.4 145,036 + +0.6 145,037 - -0.0 145,053 + +0.0 145,264 - H281DRAFT_05725 0.28 +1.5 145,264 - H281DRAFT_05725 0.28 +0.3 145,269 + H281DRAFT_05725 0.29 -1.4 145,269 + H281DRAFT_05725 0.29 +1.1 145,269 + H281DRAFT_05725 0.29 +3.0 145,269 + H281DRAFT_05725 0.29 -0.7 145,269 + H281DRAFT_05725 0.29 -0.2 145,269 + H281DRAFT_05725 0.29 +1.4 145,269 + +3.6 145,270 - H281DRAFT_05725 0.29 +0.3 145,270 - H281DRAFT_05725 0.29 -0.2 145,271 + H281DRAFT_05725 0.29 -1.1 145,272 - H281DRAFT_05725 0.29 +1.0 145,272 - H281DRAFT_05725 0.29 -0.1 145,281 + H281DRAFT_05725 0.30 +0.7 145,282 - H281DRAFT_05725 0.30 -0.5 145,282 - H281DRAFT_05725 0.30 +0.3 145,282 - H281DRAFT_05725 0.30 -0.9 145,282 - H281DRAFT_05725 0.30 +0.8 145,293 + +2.3 145,293 + H281DRAFT_05725 0.32 +0.1 145,294 - H281DRAFT_05725 0.32 -0.2 145,294 - H281DRAFT_05725 0.32 -0.6 145,294 - H281DRAFT_05725 0.32 -0.7 145,304 + H281DRAFT_05725 0.33 -2.0 145,304 + H281DRAFT_05725 0.33 +1.0 145,305 - H281DRAFT_05725 0.33 -0.6 145,331 + H281DRAFT_05725 0.37 -1.0 145,331 + H281DRAFT_05725 0.37 -0.2 145,332 - H281DRAFT_05725 0.37 +0.7 145,509 - H281DRAFT_05725 0.59 +0.6 145,542 - H281DRAFT_05725 0.64 +0.4 145,542 - H281DRAFT_05725 0.64 +0.1 145,635 - H281DRAFT_05725 0.76 -0.0 145,824 + -0.0 145,824 + -0.2 145,824 + -0.0 145,824 + +0.6 145,839 - +0.4
Or see this region's nucleotide sequence