Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05501 and H281DRAFT_05502 are separated by 96 nucleotides H281DRAFT_05502 and H281DRAFT_05503 overlap by 4 nucleotides
H281DRAFT_05501: H281DRAFT_05501 - putative spermidine/putrescine transport system substrate-binding protein, at 80,767 to 81,813
_05501
H281DRAFT_05502: H281DRAFT_05502 - transketolase subunit B, at 81,910 to 82,959
_05502
H281DRAFT_05503: H281DRAFT_05503 - transketolase subunit A, at 82,956 to 83,846
_05503
Position (kb) 81
82
83 Strain fitness (log2 ratio) -2
-1
0
1 at 80.996 kb on + strand, within H281DRAFT_05501 at 81.038 kb on + strand, within H281DRAFT_05501 at 81.260 kb on + strand, within H281DRAFT_05501 at 81.260 kb on + strand, within H281DRAFT_05501 at 81.260 kb on + strand, within H281DRAFT_05501 at 81.261 kb on - strand, within H281DRAFT_05501 at 81.261 kb on - strand, within H281DRAFT_05501 at 81.261 kb on - strand, within H281DRAFT_05501 at 81.261 kb on - strand, within H281DRAFT_05501 at 81.261 kb on - strand, within H281DRAFT_05501 at 81.476 kb on + strand, within H281DRAFT_05501 at 81.477 kb on - strand, within H281DRAFT_05501 at 81.629 kb on + strand, within H281DRAFT_05501 at 81.629 kb on + strand, within H281DRAFT_05501 at 81.630 kb on - strand, within H281DRAFT_05501 at 81.802 kb on + strand at 81.803 kb on - strand at 81.803 kb on - strand at 81.830 kb on - strand at 81.932 kb on + strand at 82.013 kb on + strand at 82.305 kb on + strand, within H281DRAFT_05502 at 82.373 kb on + strand, within H281DRAFT_05502 at 82.373 kb on + strand, within H281DRAFT_05502 at 82.374 kb on - strand, within H281DRAFT_05502 at 82.860 kb on - strand at 82.945 kb on - strand at 82.947 kb on - strand at 83.047 kb on + strand, within H281DRAFT_05503 at 83.048 kb on - strand, within H281DRAFT_05503 at 83.048 kb on - strand, within H281DRAFT_05503 at 83.252 kb on - strand, within H281DRAFT_05503 at 83.322 kb on + strand at 83.322 kb on + strand, within H281DRAFT_05503 at 83.322 kb on + strand, within H281DRAFT_05503 at 83.513 kb on - strand, within H281DRAFT_05503 at 83.517 kb on - strand, within H281DRAFT_05503 at 83.653 kb on + strand, within H281DRAFT_05503 at 83.654 kb on - strand, within H281DRAFT_05503 at 83.654 kb on - strand, within H281DRAFT_05503 at 83.670 kb on + strand, within H281DRAFT_05503 at 83.698 kb on + strand, within H281DRAFT_05503 at 83.699 kb on - strand, within H281DRAFT_05503 at 83.836 kb on - strand at 83.924 kb on - strand at 83.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 80,996 + H281DRAFT_05501 0.22 -0.3 81,038 + H281DRAFT_05501 0.26 -1.4 81,260 + H281DRAFT_05501 0.47 -2.3 81,260 + H281DRAFT_05501 0.47 +1.1 81,260 + H281DRAFT_05501 0.47 +1.0 81,261 - H281DRAFT_05501 0.47 +0.4 81,261 - H281DRAFT_05501 0.47 +0.3 81,261 - H281DRAFT_05501 0.47 -0.6 81,261 - H281DRAFT_05501 0.47 +0.3 81,261 - H281DRAFT_05501 0.47 -1.1 81,476 + H281DRAFT_05501 0.68 -0.1 81,477 - H281DRAFT_05501 0.68 +1.4 81,629 + H281DRAFT_05501 0.82 +0.7 81,629 + H281DRAFT_05501 0.82 +0.4 81,630 - H281DRAFT_05501 0.82 -0.4 81,802 + -0.5 81,803 - -0.6 81,803 - +0.3 81,830 - -0.7 81,932 + +0.9 82,013 + -1.9 82,305 + H281DRAFT_05502 0.38 +1.6 82,373 + H281DRAFT_05502 0.44 +0.2 82,373 + H281DRAFT_05502 0.44 +0.2 82,374 - H281DRAFT_05502 0.44 +0.1 82,860 - -0.1 82,945 - +0.1 82,947 - -1.1 83,047 + H281DRAFT_05503 0.10 +0.9 83,048 - H281DRAFT_05503 0.10 +0.4 83,048 - H281DRAFT_05503 0.10 +0.1 83,252 - H281DRAFT_05503 0.33 -0.5 83,322 + +0.5 83,322 + H281DRAFT_05503 0.41 -0.0 83,322 + H281DRAFT_05503 0.41 +0.3 83,513 - H281DRAFT_05503 0.63 +1.6 83,517 - H281DRAFT_05503 0.63 +0.9 83,653 + H281DRAFT_05503 0.78 +0.4 83,654 - H281DRAFT_05503 0.78 -1.1 83,654 - H281DRAFT_05503 0.78 +0.6 83,670 + H281DRAFT_05503 0.80 +0.1 83,698 + H281DRAFT_05503 0.83 +0.0 83,699 - H281DRAFT_05503 0.83 -0.3 83,836 - +0.6 83,924 - -0.7 83,945 + +0.3
Or see this region's nucleotide sequence