Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04189 and H281DRAFT_04190 are separated by 505 nucleotides H281DRAFT_04190 and H281DRAFT_04191 are separated by 266 nucleotides H281DRAFT_04191 and H281DRAFT_04192 overlap by 4 nucleotides
H281DRAFT_04189: H281DRAFT_04189 - pyruvate kinase, at 308,008 to 309,444
_04189
H281DRAFT_04190: H281DRAFT_04190 - phosphoglycerate kinase, at 309,950 to 311,143
_04190
H281DRAFT_04191: H281DRAFT_04191 - Branched-chain amino acid transport protein, at 311,410 to 311,733
_04191
H281DRAFT_04192: H281DRAFT_04192 - Predicted branched-chain amino acid permease (azaleucine resistance), at 311,730 to 312,476
_04192
Position (kb) 309
310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 309.952 kb on - strand at 309.969 kb on - strand at 310.036 kb on - strand at 310.036 kb on - strand at 311.313 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.411 kb on + strand at 311.412 kb on - strand at 311.513 kb on + strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.514 kb on - strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.563 kb on + strand, within H281DRAFT_04191 at 311.564 kb on - strand, within H281DRAFT_04191 at 311.766 kb on + strand at 312.064 kb on + strand, within H281DRAFT_04192 at 312.064 kb on + strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.065 kb on - strand, within H281DRAFT_04192 at 312.108 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.121 kb on + strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.122 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192 at 312.143 kb on - strand, within H281DRAFT_04192
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 309,952 - -3.5 309,969 - -0.0 310,036 - +1.0 310,036 - -0.6 311,313 + -0.6 311,411 + +0.2 311,411 + +1.2 311,411 + +1.0 311,411 + -0.1 311,412 - +0.8 311,513 + H281DRAFT_04191 0.32 +0.2 311,514 - H281DRAFT_04191 0.32 -2.1 311,514 - H281DRAFT_04191 0.32 -0.5 311,514 - H281DRAFT_04191 0.32 -2.1 311,514 - H281DRAFT_04191 0.32 +1.7 311,563 + H281DRAFT_04191 0.47 -3.4 311,563 + H281DRAFT_04191 0.47 -1.7 311,564 - H281DRAFT_04191 0.48 +0.4 311,766 + -0.9 312,064 + H281DRAFT_04192 0.45 -3.2 312,064 + H281DRAFT_04192 0.45 +1.3 312,065 - H281DRAFT_04192 0.45 -0.1 312,065 - H281DRAFT_04192 0.45 -0.5 312,065 - H281DRAFT_04192 0.45 -1.0 312,065 - H281DRAFT_04192 0.45 +0.3 312,108 + H281DRAFT_04192 0.51 -0.2 312,121 + H281DRAFT_04192 0.52 -1.6 312,121 + H281DRAFT_04192 0.52 -0.7 312,121 + H281DRAFT_04192 0.52 -1.1 312,121 + H281DRAFT_04192 0.52 -1.1 312,121 + H281DRAFT_04192 0.52 -0.9 312,122 - H281DRAFT_04192 0.52 -0.6 312,122 - H281DRAFT_04192 0.52 -1.4 312,122 - H281DRAFT_04192 0.52 -1.2 312,122 - H281DRAFT_04192 0.52 -0.4 312,122 - H281DRAFT_04192 0.52 -0.1 312,143 - H281DRAFT_04192 0.55 -2.1 312,143 - H281DRAFT_04192 0.55 -0.7
Or see this region's nucleotide sequence