Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03907 and H281DRAFT_03908 overlap by 35 nucleotides H281DRAFT_03908 and H281DRAFT_03909 are separated by 55 nucleotides H281DRAFT_03909 and H281DRAFT_03910 are separated by 155 nucleotides
H281DRAFT_03907: H281DRAFT_03907 - Lysophospholipase, alpha-beta hydrolase superfamily, at 3,622 to 4,575
_03907
H281DRAFT_03908: H281DRAFT_03908 - Enoyl-CoA hydratase/carnithine racemase, at 4,541 to 5,341
_03908
H281DRAFT_03909: H281DRAFT_03909 - Acetoin utilization deacetylase AcuC, at 5,397 to 6,371
_03909
H281DRAFT_03910: H281DRAFT_03910 - membrane-bound lytic murein transglycosylase B, at 6,527 to 7,765
_03910
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4.444 kb on + strand, within H281DRAFT_03907 at 4.444 kb on + strand, within H281DRAFT_03907 at 4.445 kb on - strand, within H281DRAFT_03907 at 4.726 kb on + strand, within H281DRAFT_03908 at 4.727 kb on - strand, within H281DRAFT_03908 at 4.966 kb on + strand, within H281DRAFT_03908 at 4.966 kb on + strand, within H281DRAFT_03908 at 4.966 kb on + strand, within H281DRAFT_03908 at 4.967 kb on - strand, within H281DRAFT_03908 at 5.082 kb on + strand, within H281DRAFT_03908 at 5.085 kb on + strand, within H281DRAFT_03908 at 5.289 kb on + strand at 5.378 kb on + strand at 5.458 kb on + strand at 5.459 kb on - strand at 5.515 kb on - strand, within H281DRAFT_03909 at 5.540 kb on - strand, within H281DRAFT_03909 at 5.540 kb on - strand, within H281DRAFT_03909 at 5.715 kb on - strand, within H281DRAFT_03909 at 5.715 kb on - strand, within H281DRAFT_03909 at 5.715 kb on - strand, within H281DRAFT_03909 at 6.036 kb on + strand, within H281DRAFT_03909 at 6.053 kb on + strand, within H281DRAFT_03909 at 6.121 kb on + strand, within H281DRAFT_03909 at 6.150 kb on + strand, within H281DRAFT_03909 at 6.150 kb on + strand, within H281DRAFT_03909 at 6.150 kb on + strand, within H281DRAFT_03909 at 6.150 kb on + strand, within H281DRAFT_03909 at 6.151 kb on - strand, within H281DRAFT_03909 at 6.304 kb on + strand at 6.304 kb on + strand at 6.305 kb on - strand at 6.305 kb on - strand at 6.305 kb on - strand at 6.305 kb on - strand at 6.411 kb on + strand at 6.412 kb on - strand at 6.412 kb on - strand at 6.412 kb on - strand at 6.429 kb on + strand at 6.430 kb on - strand at 6.526 kb on + strand at 6.527 kb on - strand at 6.527 kb on - strand at 6.778 kb on + strand, within H281DRAFT_03910 at 6.792 kb on - strand, within H281DRAFT_03910 at 6.840 kb on - strand, within H281DRAFT_03910 at 7.094 kb on - strand, within H281DRAFT_03910 at 7.124 kb on - strand, within H281DRAFT_03910 at 7.169 kb on + strand, within H281DRAFT_03910 at 7.169 kb on + strand, within H281DRAFT_03910 at 7.169 kb on + strand, within H281DRAFT_03910 at 7.170 kb on - strand, within H281DRAFT_03910 at 7.170 kb on - strand, within H281DRAFT_03910 at 7.223 kb on + strand, within H281DRAFT_03910 at 7.224 kb on - strand, within H281DRAFT_03910 at 7.286 kb on + strand, within H281DRAFT_03910 at 7.287 kb on - strand, within H281DRAFT_03910 at 7.352 kb on + strand, within H281DRAFT_03910 at 7.353 kb on - strand, within H281DRAFT_03910 at 7.353 kb on - strand, within H281DRAFT_03910
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 4,444 + H281DRAFT_03907 0.86 +0.0 4,444 + H281DRAFT_03907 0.86 -0.0 4,445 - H281DRAFT_03907 0.86 +0.3 4,726 + H281DRAFT_03908 0.23 +0.8 4,727 - H281DRAFT_03908 0.23 -0.8 4,966 + H281DRAFT_03908 0.53 +1.5 4,966 + H281DRAFT_03908 0.53 +0.7 4,966 + H281DRAFT_03908 0.53 +2.0 4,967 - H281DRAFT_03908 0.53 -0.4 5,082 + H281DRAFT_03908 0.68 +0.4 5,085 + H281DRAFT_03908 0.68 +0.0 5,289 + -1.1 5,378 + +0.0 5,458 + +2.0 5,459 - -0.4 5,515 - H281DRAFT_03909 0.12 -1.5 5,540 - H281DRAFT_03909 0.15 -0.2 5,540 - H281DRAFT_03909 0.15 -0.6 5,715 - H281DRAFT_03909 0.33 -0.1 5,715 - H281DRAFT_03909 0.33 -2.4 5,715 - H281DRAFT_03909 0.33 -2.1 6,036 + H281DRAFT_03909 0.66 +0.9 6,053 + H281DRAFT_03909 0.67 +0.2 6,121 + H281DRAFT_03909 0.74 -0.4 6,150 + H281DRAFT_03909 0.77 -1.1 6,150 + H281DRAFT_03909 0.77 -2.2 6,150 + H281DRAFT_03909 0.77 +0.0 6,150 + H281DRAFT_03909 0.77 +0.1 6,151 - H281DRAFT_03909 0.77 -0.9 6,304 + -1.0 6,304 + +0.2 6,305 - -0.8 6,305 - +0.2 6,305 - -1.2 6,305 - +0.1 6,411 + -2.8 6,412 - +0.2 6,412 - -0.3 6,412 - +0.7 6,429 + +0.2 6,430 - -0.6 6,526 + +1.1 6,527 - +1.5 6,527 - +0.2 6,778 + H281DRAFT_03910 0.20 +1.7 6,792 - H281DRAFT_03910 0.21 +1.0 6,840 - H281DRAFT_03910 0.25 +0.5 7,094 - H281DRAFT_03910 0.46 +0.9 7,124 - H281DRAFT_03910 0.48 +0.6 7,169 + H281DRAFT_03910 0.52 +0.2 7,169 + H281DRAFT_03910 0.52 -0.2 7,169 + H281DRAFT_03910 0.52 -1.0 7,170 - H281DRAFT_03910 0.52 +0.5 7,170 - H281DRAFT_03910 0.52 +0.4 7,223 + H281DRAFT_03910 0.56 -0.0 7,224 - H281DRAFT_03910 0.56 +1.6 7,286 + H281DRAFT_03910 0.61 +0.8 7,287 - H281DRAFT_03910 0.61 +0.3 7,352 + H281DRAFT_03910 0.67 -0.3 7,353 - H281DRAFT_03910 0.67 +0.8 7,353 - H281DRAFT_03910 0.67 +1.2
Or see this region's nucleotide sequence