Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03139 and H281DRAFT_03140 are separated by 210 nucleotides H281DRAFT_03140 and H281DRAFT_03141 are separated by 82 nucleotides H281DRAFT_03141 and H281DRAFT_03142 are separated by 266 nucleotides
H281DRAFT_03139: H281DRAFT_03139 - membrane protein, at 176,316 to 177,641
_03139
H281DRAFT_03140: H281DRAFT_03140 - Cys-tRNA(Pro) deacylase, prolyl-tRNA editing enzyme YbaK/EbsC, at 177,852 to 178,391
_03140
H281DRAFT_03141: H281DRAFT_03141 - sulfate permease, SulP family, at 178,474 to 179,961
_03141
H281DRAFT_03142: H281DRAFT_03142 - Lipid-binding SYLF domain-containing protein, at 180,228 to 180,818
_03142
Position (kb)
178
179
180 Strain fitness (log2 ratio)
-1
0
1
2
3 at 177.661 kb on - strand at 177.747 kb on + strand at 177.747 kb on + strand at 177.748 kb on - strand at 177.748 kb on - strand at 177.749 kb on + strand at 177.750 kb on - strand at 177.750 kb on - strand at 177.811 kb on + strand at 177.827 kb on - strand at 177.835 kb on + strand at 177.835 kb on + strand at 177.836 kb on - strand at 177.836 kb on - strand at 177.836 kb on - strand at 177.842 kb on - strand at 177.842 kb on - strand at 178.040 kb on + strand, within H281DRAFT_03140 at 178.041 kb on - strand, within H281DRAFT_03140 at 178.041 kb on - strand, within H281DRAFT_03140 at 178.057 kb on - strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.080 kb on + strand, within H281DRAFT_03140 at 178.081 kb on - strand, within H281DRAFT_03140 at 178.218 kb on - strand, within H281DRAFT_03140 at 178.516 kb on - strand at 178.516 kb on - strand at 178.718 kb on + strand, within H281DRAFT_03141 at 178.718 kb on + strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.719 kb on - strand, within H281DRAFT_03141 at 178.725 kb on + strand, within H281DRAFT_03141 at 178.725 kb on + strand, within H281DRAFT_03141 at 179.003 kb on + strand, within H281DRAFT_03141 at 179.003 kb on + strand, within H281DRAFT_03141 at 179.004 kb on - strand, within H281DRAFT_03141 at 179.249 kb on + strand, within H281DRAFT_03141 at 179.249 kb on + strand, within H281DRAFT_03141 at 179.352 kb on + strand, within H281DRAFT_03141 at 179.442 kb on - strand, within H281DRAFT_03141 at 179.668 kb on + strand, within H281DRAFT_03141 at 179.688 kb on - strand, within H281DRAFT_03141 at 179.944 kb on - strand at 179.956 kb on - strand at 180.404 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.505 kb on + strand, within H281DRAFT_03142 at 180.506 kb on - strand at 180.506 kb on - strand, within H281DRAFT_03142 at 180.649 kb on + strand at 180.821 kb on + strand at 180.822 kb on - strand at 180.822 kb on - strand at 180.833 kb on + strand at 180.833 kb on + strand at 180.833 kb on + strand at 180.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 177,661 - +0.2 177,747 + +0.3 177,747 + -1.5 177,748 - -0.1 177,748 - -0.5 177,749 + +0.4 177,750 - -0.1 177,750 - -0.5 177,811 + -0.1 177,827 - -0.6 177,835 + +0.2 177,835 + -0.2 177,836 - +1.6 177,836 - +0.6 177,836 - +1.0 177,842 - -1.0 177,842 - +0.2 178,040 + H281DRAFT_03140 0.35 +0.4 178,041 - H281DRAFT_03140 0.35 +0.6 178,041 - H281DRAFT_03140 0.35 +0.8 178,057 - H281DRAFT_03140 0.38 +0.5 178,080 + H281DRAFT_03140 0.42 +1.2 178,080 + H281DRAFT_03140 0.42 -0.3 178,080 + H281DRAFT_03140 0.42 +0.6 178,081 - H281DRAFT_03140 0.42 -0.6 178,218 - H281DRAFT_03140 0.68 +0.9 178,516 - -1.6 178,516 - +3.1 178,718 + H281DRAFT_03141 0.16 +1.8 178,718 + H281DRAFT_03141 0.16 -0.4 178,719 - H281DRAFT_03141 0.16 -1.0 178,719 - H281DRAFT_03141 0.16 -0.2 178,719 - H281DRAFT_03141 0.16 -1.1 178,719 - H281DRAFT_03141 0.16 -0.2 178,719 - H281DRAFT_03141 0.16 +0.3 178,725 + H281DRAFT_03141 0.17 -0.9 178,725 + H281DRAFT_03141 0.17 -0.6 179,003 + H281DRAFT_03141 0.36 -0.3 179,003 + H281DRAFT_03141 0.36 -0.5 179,004 - H281DRAFT_03141 0.36 -0.1 179,249 + H281DRAFT_03141 0.52 -0.4 179,249 + H281DRAFT_03141 0.52 -1.4 179,352 + H281DRAFT_03141 0.59 -0.4 179,442 - H281DRAFT_03141 0.65 -0.2 179,668 + H281DRAFT_03141 0.80 +0.6 179,688 - H281DRAFT_03141 0.82 +1.1 179,944 - +0.2 179,956 - -0.6 180,404 + H281DRAFT_03142 0.30 +0.8 180,505 + H281DRAFT_03142 0.47 +1.2 180,505 + H281DRAFT_03142 0.47 -1.6 180,505 + H281DRAFT_03142 0.47 +0.0 180,506 - -1.2 180,506 - H281DRAFT_03142 0.47 +0.5 180,649 + +0.8 180,821 + -0.5 180,822 - -0.4 180,822 - +0.6 180,833 + +0.2 180,833 + +0.1 180,833 + -1.0 180,833 + -0.3
Or see this region's nucleotide sequence