Experiment: 2'-Deoxyinosine 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03117 and H281DRAFT_03118 overlap by 8 nucleotides H281DRAFT_03118 and H281DRAFT_03119 overlap by 4 nucleotides H281DRAFT_03119 and H281DRAFT_03120 are separated by 23 nucleotides
H281DRAFT_03117: H281DRAFT_03117 - sarcosine oxidase subunit beta, at 150,084 to 151,211
_03117
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
H281DRAFT_03119: H281DRAFT_03119 - NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G), at 152,634 to 153,002
_03119
H281DRAFT_03120: H281DRAFT_03120 - putative spermidine/putrescine transport system permease protein, at 153,026 to 153,820
_03120
Position (kb) 151
152
153 Strain fitness (log2 ratio) -1
0
1 at 150.656 kb on + strand, within H281DRAFT_03117 at 151.056 kb on + strand, within H281DRAFT_03117 at 151.250 kb on + strand at 151.251 kb on - strand at 151.251 kb on - strand at 151.456 kb on + strand, within H281DRAFT_03118 at 152.009 kb on + strand, within H281DRAFT_03118 at 152.230 kb on - strand, within H281DRAFT_03118 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.160 kb on - strand, within H281DRAFT_03120 at 153.397 kb on + strand, within H281DRAFT_03120
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 10 mM (C) remove 150,656 + H281DRAFT_03117 0.51 -0.1 151,056 + H281DRAFT_03117 0.86 -0.2 151,250 + -1.6 151,251 - -1.5 151,251 - +0.4 151,456 + H281DRAFT_03118 0.18 -1.2 152,009 + H281DRAFT_03118 0.56 -1.2 152,230 - H281DRAFT_03118 0.72 -0.0 153,158 - H281DRAFT_03120 0.17 -0.6 153,158 - H281DRAFT_03120 0.17 -0.8 153,160 - H281DRAFT_03120 0.17 +0.7 153,397 + H281DRAFT_03120 0.47 +0.8
Or see this region's nucleotide sequence